Novo human insulin - the same but different

1990 ◽  
Vol 28 (6) ◽  
pp. 24-24

Unannounced to prescribers or patients, Novo Nordisk have changed the manufacturing process of their human insulins from enzymatic modification of pig insulin (emp) to direct biosynthesis of genetically engineered yeast cells (pyr). The preservative for Human Actrapid insulin has also been changed from phenol to meta-cresol. No warning of this change was sent to pharmacists, doctors, diabetes specialists or patients.

2018 ◽  
Vol 28 (4) ◽  
pp. 190-200 ◽  
Author(s):  
Yan-Feng Li ◽  
Hong Jiang ◽  
Zhong Hu ◽  
Guang-Lei Liu ◽  
Zhen-Ming Chi ◽  
...  

In this study, in order to directly and efficiently convert inulin into a single-cell oil (SCO), an <i>INU1</i> gene encoding inulinase from<b><i></i></b> <i>Kluyveromyces marxianus</i> was integrated into the genomic DNA and actively expressed in an SCO producer <i>Aureobasidium</i> <i>melanogenum</i> P10. The transformant API41 obtained produced 28.5 U/mL of inulinase and its wild-type strain P10 yielded only 8.62 U/mL. Most (97.5%) of the inulinase produced by the transformant API41 was secreted into the culture. During a 10-L fermentation, 66.2% (w/w) lipid in the yeast cells of the transformant API41 and 14.38 g/L of cell dry weight were attained from inulin of 80.0 g/L within 120 h, high inulinase activity (23.7 U/mL) was also produced within 72 h, and the added inulin was actively hydrolyzed. This confirmed that the genetically engineered yeast of <i>A. melanogenum</i> P10 is suitable for direct production of lipids from inulin. The lipids produced could be used as feedstocks for biodiesel production.


2009 ◽  
Vol 30 (19) ◽  
pp. 3406-3412 ◽  
Author(s):  
Kosuke Ino ◽  
Yusuke Kitagawa ◽  
Tsuyoshi Watanabe ◽  
Hitoshi Shiku ◽  
Masahiro Koide ◽  
...  

1995 ◽  
Vol 82-83 ◽  
pp. 815-822 ◽  
Author(s):  
Denis Pompon ◽  
Alain Perret ◽  
Aouatef Bellamine ◽  
Romuald Laine ◽  
Jean-Charles Gautier ◽  
...  

2007 ◽  
Vol 99 (2) ◽  
pp. 333-340 ◽  
Author(s):  
Shailendra Singh ◽  
Wonkyu Lee ◽  
Nancy A. DaSilva ◽  
Ashok Mulchandani ◽  
Wilfred Chen

2000 ◽  
Vol 66 (12) ◽  
pp. 5477-5479 ◽  
Author(s):  
Marina Vai ◽  
Luca Brambilla ◽  
Ivan Orlandi ◽  
Nicola Rota ◽  
Bianca Maria Ranzi ◽  
...  

ABSTRACT We studied the secretion of recombinant human insulin-like growth factor 1 (rhIGF-1) from transformed yeast cells. The hIGF-1gene was fused to the mating factor α prepro- leader sequence under the control of the constitutive ACT1 promoter. We found that the inactivation of the GAS1 gene in the host strain led to a supersecretory phenotype yielding a considerable increase, from 8 to 55 mg/liter, in rhIGF-1 production.


Yeast ◽  
1990 ◽  
Vol 6 (4) ◽  
pp. 319-330 ◽  
Author(s):  
Leda M. Borralho ◽  
Claudio H. D. Ortiz ◽  
Anita D. Panek ◽  
James R. Mattoon

2014 ◽  
Vol 446 ◽  
pp. 37-43 ◽  
Author(s):  
Jun Ishii ◽  
Asami Oda ◽  
Shota Togawa ◽  
Akira Fukao ◽  
Toshinobu Fujiwara ◽  
...  

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