Optimization of transient gene expression in pollen of Norway spruce (Picea abies) by particle acceleration

1994 ◽  
Vol 92 (3) ◽  
pp. 412-416 ◽  
Author(s):  
Inger Martinussen ◽  
Olavi Junttila ◽  
David Twell
Keyword(s):  
Gene Expression ◽  
Particle Acceleration ◽  
Picea Abies ◽  
Norway Spruce ◽  
Transient Gene Expression

scholarly journals Disentangling the Roles of History and Local Selection in Shaping Clinal Variation of Allele Frequencies and Gene Expression in Norway Spruce (Picea abies)

Genetics ◽  
2012 ◽  
Vol 191 (3) ◽  
pp. 865-881 ◽  
Author(s):  
Jun Chen ◽  
Thomas Källman ◽  
Xiaofei Ma ◽  
Niclas Gyllenstrand ◽  
Giusi Zaina ◽  
...  
Keyword(s):  
Gene Expression ◽  
Picea Abies ◽  
Norway Spruce ◽  
Allele Frequencies ◽  
Clinal Variation ◽  
Local Selection

scholarly journals Growth and Physiological Responses of Norway Spruce (Picea abies (L.) H. Karst) Supplemented with Monochromatic Red, Blue and Far-Red Light

Forests ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 164
Author(s):  
Fangqun OuYang ◽  
Yang Ou ◽  
Tianqin Zhu ◽  
Jianwei Ma ◽  
Sanping An ◽  
...  
Keyword(s):  
Gene Expression ◽  
Electron Transport ◽  
Picea Abies ◽  
Norway Spruce ◽  
Light Quality ◽  
Physiological Responses ◽  
Red Light ◽  
Variable Fluorescence ◽  
Related Gene ◽  
Bisphosphate Carboxylase

Monochromatic red light (R) supplementation is more efficient than blue light (B) in promoting Norway spruce (Picea abies (L.) H. Karst) growth. Transcriptome analysis has revealed that R and B may regulate stem growth by regulating phytohormones and secondary metabolites; however, the effects of light qualities on physiological responses and related gene expression in Norway spruce require further study. In the present study, three-year-old Norway spruce seedlings received sunlight during the daytime were exposed to monochromatic B (460 mm), monochromatic R (660 nm), monochromatic far-red light (FR, 730 nm), and a combination of three monochromatic lights (control, R:FR:B = 7:1:1) using light-emitting diode (LED) lamps for 12 h after sunset for 90 day. Growth traits, physiological responses, and related gene expression were determined. The results showed that light quality significantly affected Norway spruce growth. The stem height, root collar diameter, and current-year shoot length of seedlings treated with R were 2%, 10% and 12% higher, respectively, than those of the control, whereas seedlings treated with B and FR showed significantly lower values of these parameters compared with that of the control. The net photosynthetic rate (Pn) of seedlings under R treatment was 10% higher than that of the control, whereas the Pn values of seedlings treated with FR and B were 22% and 33%, respectively, lower than that of the control. The ratio of phosphoenolpyruvate carboxylase to ribulose-1,5-bisphosphate carboxylase/oxygenase (PEPC/Rubisco) of seedlings after the R treatment (0.581) was the highest and 3.98 times higher than that of the seedlings treated with B. Light quality significantly affected the gibberellic acid (GAs) levels, which was 13% higher in seedlings treated with R (6.4 g/100 ng) than that of the control, whereas, the GAs level of seedlings treated with B and FR was 17% and 19% lower, respectively, than that of the control. In addition, seedlings treated with R achieved the lowest ratio of leaf chlorophyll content to fresh weight (8.7). Compared to the R and control treatments, seedlings received FR treatment had consistently lower values of the quantum yield of electron transport beyond QA− (primary quinone, ϕEo) and efficiency, with which a trapped exciton moves an electron into the electron transport chain beyond QA− (ψo), while higher values of the relatively variable fluorescence at the J step and normalized relatively variable fluorescence at the K step (Wk). The values of ϕEo, ψO, VJ and Wk in seedlings treated with B were similar to those in the control group. The expression of genes associated with light signal transduction, such as PHYTOCHROME C (PHYC), ELONGATED HYPOCOTYL5 (HY5), CONSTITUTIVE PHOTOMORPHOGENIC 1-2 (COP1-2), and PHYTOCHROMEINTERACTING FACTOR 3 (PIF3), was significantly higher in seedlings under B treatment than those under other light treatments. Nevertheless, significant differences were not observed in the expression of COP1-2, HY5, and PIF3 between the R treatment and the control. The expression value of COP1-2 was significantly lower in R than FR light treatments. In conclusion, compared with the control, R promotes, whereas B and FR inhibit Norway spruce growth, which was accompanied by physiological changes and genes expression regulation that may be relate to a changing phytochrome photostationary state (PSS) with the supplemental R in seedlings.

Factors influencing transient gene expression in bean (Phaseolus vulgaris L.) using an electrical particle acceleration device

1993 ◽  
Vol 12 (9) ◽  
pp. 483-490 ◽  
Author(s):  
F. J. L. Aragao ◽  
M. -F. Grossi de Sa ◽  
M. R. Davey ◽  
A. C. M. Brasileiro ◽  
J. C. Faria ◽  
...  
Keyword(s):  
Gene Expression ◽  
Particle Acceleration ◽  
Phaseolus Vulgaris ◽  
Transient Gene Expression ◽  
Phaseolus Vulgaris L ◽  
Factors Influencing ◽  
Electrical Particle
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