Alterations of cellular characteristics of a human ovarian teratocarcinoma cell line after in vitro treatment with retinoids

1990 ◽  
Vol 43 (2) ◽  
pp. 123-130 ◽  
Author(s):  
Douglas D. Taylor ◽  
Cicek Gercel Taylor ◽  
Paul H. Black ◽  
Cheng-gan Jiang ◽  
Iih-Nan Chou
Keyword(s):  
Cell Line ◽  
Teratocarcinoma Cell ◽  
Teratocarcinoma Cell Line ◽  
In Vitro Treatment

Retinoic acid treatment enhances the acetylcholine contents in the human teratocarcinoma cell line NTera-2

2000 ◽  
Vol 96 (1-2) ◽  
pp. 59-63 ◽  
Author(s):  
Hanspeter S. Fischer ◽  
Irene Berti ◽  
Dieter S. Schatz ◽  
Christian Humpel ◽  
Alois Saria
Keyword(s):  
Retinoic Acid ◽  
Cell Line ◽  
Acid Treatment ◽  
Retinoic Acid Treatment ◽  
Teratocarcinoma Cell ◽  
Teratocarcinoma Cell Line

Quercetin reduces pluripotency, migration and adhesion of human teratocarcinoma cell line NT2/D1 by inhibiting Wnt/β-catenin signaling

2014 ◽  
Vol 5 (10) ◽  
pp. 2564-2573 ◽  
Author(s):  
Marija Mojsin ◽  
Jelena Marjanovic Vicentic ◽  
Marija Schwirtlich ◽  
Vladanka Topalovic ◽  
Milena Stevanovic
Keyword(s):  
Wnt Signaling ◽  
Cell Line ◽  
Anticancer Effect ◽  
Teratocarcinoma Cell ◽  
Teratocarcinoma Cell Line

In human teratocarcinoma cell line NT2/D1 quercetin exerts its anticancer effect through the inhibition of Wnt signaling.

The FRAME Alternatives Laboratory Database. 1. In Vitro Basal Cytotoxicity determined by the Kenacid Blue Total Protein Assay

2006 ◽  
Vol 34 (2) ◽  
pp. 151-175 ◽  
Author(s):  
Richard Clothier ◽  
Elke Gottschalg ◽  
Silvia Casati ◽  
Michael Balls
Keyword(s):  
Cell Line ◽  
Total Protein ◽  
Cell Number ◽  
Cellular Protein ◽  
Teratocarcinoma Cell ◽  
Wide Range ◽  
Significant Difference ◽  
Order Of Magnitude ◽  
Basal Cytotoxicity

A database of over 280 chemicals has been compiled by using a mouse 3T3-L1 fibroblast-like cell line in exponential growth, exposed to chemicals for 72 hours in a 96-well tissue culture plate format, and determining cell number via the Kenacid blue (KB) assay for total protein. Ranking the chemicals according to their basal cytotoxicity, expressed as the concentration (mM) that inhibits increase in total cellular protein over 72 hours by 50% (the ID50 value) shows a wide range of ID50 values, from 0.00003mM to 10,096mM. This information includes the results for MEIC chemicals 1–50, and we have now added basal cytotoxicity data for 23 of the next 25 MEIC chemicals. When the neutral red uptake (NRU) assay was performed with the same cell cultures, before the KB assay, very similar indications of basal cytotoxicity were obtained. Comparisons between the results with 3T3-L1 cells and with a human fibroblast-like cell line, BCL-D1 showed a significant difference in order of magnitude of the ID50 value for only 5 of 52 chemicals. However, there was a difference in ID50 value of more than one order of magnitude for 8 of 24 chemicals tested with an undifferentiated teratocarcinoma cell line, F9.

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