Tissue-specific RNA interference in post-implantation mouse embryos using directional electroporation and whole embryo culture

2004 ◽  
Vol 72 (2-3) ◽  
pp. 92-102 ◽  
Author(s):  
Federico Calegari ◽  
Anne-Marie Marzesco ◽  
Ralf Kittler ◽  
Frank Buchholz ◽  
Wieland B. Huttner
Keyword(s):  
Rna Interference ◽  
Embryo Culture ◽  
Mouse Embryos ◽  
Whole Embryo Culture ◽  
Tissue Specific ◽  
Post Implantation

scholarly journals Whole embryo culture, transcriptomics and RNA interference identify TBX1 and FGF11 as novel regulators of limb development in the mouse

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Gautier Tejedor ◽  
Béryl Laplace-Builhé ◽  
Patricia Luz-Crawford ◽  
Said Assou ◽  
Audrey Barthelaix ◽  
...  
Keyword(s):  
Rna Interference ◽  
Embryo Culture ◽  
Limb Development ◽  
Whole Embryo Culture

Improved growth and development of presomite mouse embryos in whole embryo culture

1988 ◽  
Vol 245 (3) ◽  
pp. 264-269 ◽  
Author(s):  
E. S. Hunter ◽  
W. Balkan ◽  
T. W. Sadler
Keyword(s):  
Embryo Culture ◽  
Growth And Development ◽  
Mouse Embryos ◽  
Whole Embryo Culture

scholarly journals Cell death in rat and mouse embryos exposed to methanol in whole embryo culture

Toxicology ◽  
1995 ◽  
Vol 97 (1-3) ◽  
pp. 159-171 ◽  
Author(s):  
B.D. Abbott ◽  
M. Ebron-mccoy ◽  
J.E. Andrews
Keyword(s):  
Cell Death ◽  
Embryo Culture ◽  
Mouse Embryos ◽  
Whole Embryo Culture

scholarly journals Tissue-specific RNA interference in postimplantation mouse embryos with endoribonuclease-prepared short interfering RNA

2002 ◽  
Vol 99 (22) ◽  
pp. 14236-14240 ◽  
Author(s):  
F. Calegari ◽  
W. Haubensak ◽  
D. Yang ◽  
W. B. Huttner ◽  
F. Buchholz
Keyword(s):  
Rna Interference ◽  
Mouse Embryos ◽  
Short Interfering Rna ◽  
Tissue Specific ◽  
Interfering Rna

scholarly journals Postimplantation Whole Embryo Culture Assay for Hamsters: An Alternative to Rat and Mouse

2001 ◽  
Vol 1 ◽  
pp. 227-234 ◽  
Author(s):  
Bogdan Wlodarczyk ◽  
Bogumil Biernacki ◽  
Maria Minta ◽  
Jan Zmudzki
Keyword(s):  
Embryo Culture ◽  
Culture Conditions ◽  
Mouse Embryos ◽  
Total Protein Content ◽  
Image Analysis System ◽  
Whole Embryo Culture ◽  
Analysis System ◽  
Comparison Of The Results ◽  
Rats And Mice

Postimplantation whole embryo culture (WEC) assay for rats and mice has been well established and introduced to many laboratories. Recently WEC technique for rabbits has been developed; however, information on culture of other species is very limited. Knowing the usefulness of hamsters in classical embryotoxicology, we reasoned that hamster WEC could be an alternative model for the most frequently used rat and mouse WEC. Previously we have optimized culture conditions for postimplantation hamster embryos. The aim of this study was to test the susceptibility of hamster embryos cultured in vitro to embryotoxic compounds and to compare our results with those reported by others on rat or mouse embryo culture. For that purpose we choose three known embryotoxic compounds�valproic acid, cadmium chloride, and diethylstilbestrol�and tested them using a postimplantation hamster whole embryo culture assay. Hamster embryos were cultured from 7.5 days gestation for 24 h in a medium consisting of 35% hamster serum and 65% synthetic culture medium (Iscove�s or McCoy 5A). At the end of the culture period, the embryos were examined morphologically, measured with the aid of a computer image analysis system, and total protein content was assessed. All three compounds exhibited dose-related embryotoxic and teratogenic effects in hamster embryos. The malformations observed were similar to those reported on rat and mouse embryos. Comparison of the results with data reported by other authors indicates that hamster embryos cultured in vitromight be more susceptible to embryotoxic stimuli than rat and mouse embryos.

Whole-embryo culture of E5.5 mouse embryos: Development to the gastrulation stage

genesis ◽  
2003 ◽  
Vol 37 (1) ◽  
pp. 38-43 ◽  
Author(s):  
Shigeto Miura ◽  
Yuji Mishina
Keyword(s):  
Embryo Culture ◽  
Mouse Embryos ◽  
Whole Embryo Culture

Post-implantation whole embryo culture and the study of teratogenesis

1986 ◽  
Vol 24 (6-7) ◽  
pp. 619-622 ◽  
Author(s):  
S.J. Freeman ◽  
C.E. Steele
Keyword(s):  
Embryo Culture ◽  
Whole Embryo Culture ◽  
Post Implantation

Whole embryo culture of presomitic mouse embryos

1995 ◽  
Vol 9 (5) ◽  
pp. 671-675 ◽  
Author(s):  
G. Van Maele-fabry ◽  
F. Gofflot ◽  
J.J. Picard
Keyword(s):  
Embryo Culture ◽  
Mouse Embryos ◽  
Whole Embryo Culture

scholarly journals Embryotoxicity of Transgenic Rice TT51 and Cry1Ab Bt Insecticidal Toxin in Rat Post Implantation Whole Embryo Culture

2014 ◽  
Vol 2 (3) ◽  
pp. 115-121
Author(s):  
Qianying Guo ◽  
Shuangjia Wang ◽  
Yu Wang ◽  
Liren Wei ◽  
Han Zhu ◽  
...  
Keyword(s):  
Transgenic Rice ◽  
Embryo Culture ◽  
Insecticidal Toxin ◽  
Whole Embryo Culture ◽  
Post Implantation

scholarly journals ­­Highly Conducive Ex utero Mouse Embryogenesis from Pre-Gastrulation to Late Organogenesis

2021 ◽  
Author(s):  
Alejandro Aguilera-Castrejon ◽  
Jacob H. Hanna
Keyword(s):  
Mouse Embryo ◽  
Embryo Culture ◽  
Hind Limb ◽  
Normal Mouse ◽  
Mouse Embryos ◽  
Formation Stage ◽  
Stable Growth ◽  
Post Implantation ◽  
Mouse Embryo Culture

Abstract In mammals, morphogenesis and organogenesis take place after the embryo implants into the uterus, which makes it relatively inaccessible for observation and manipulation. While methods for in vitro culture of pre- and peri-implantation mouse embryos are routinely used, approaches for efficient and stable culture of post-implantation embryos from egg cylinder stages until advanced organogenesis remain to be established. We recently developed highly robust ex utero post-implantation mouse embryo culture platforms, that enable appropriate and faithful development of embryos before gastrulation (E5.5) until the hind limb formation stage (E11). In these protocols, late gastrulating embryos (E7.5) are grown in 3D rotating bottles settings, while extended culture from pre-gastrulation stages (E5.5 or E6.5) requires a combination of static and rotating bottle culture protocols. These systems support stable growth of normal mouse embryos ex utero from pre-gastrulation to advanced organogenesis.

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