Improved growth and development of presomite mouse embryos in whole embryo culture

1988 ◽  
Vol 245 (3) ◽  
pp. 264-269 ◽  
Author(s):  
E. S. Hunter ◽  
W. Balkan ◽  
T. W. Sadler
Keyword(s):  
Embryo Culture ◽  
Growth And Development ◽  
Mouse Embryos ◽  
Whole Embryo Culture

Teratogenicity of Staphylococcus aureus L-forms using a mouse whole-embryo culture model

2013 ◽  
Vol 62 (5) ◽  
pp. 677-682
Author(s):  
Yong Liu ◽  
Xiang Zhu ◽  
Feng-ling Yu ◽  
Xiao-ming Kong ◽  
Na Lin ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Growth And Development ◽  
Mouse Embryos ◽  
Head Length ◽  
Control Group ◽  
Whole Embryo Culture ◽  
Crown Rump Length ◽  
Gram Staining ◽  
Pass Through

Our previous studies have suggested that Staphylococcus aureus L-forms are able to pass through the placental barrier of mice from the maternal side to the fetal body and affect fetal growth and development, but little is known about the direct influence of S. aureus L-forms on embryos during the critical period of organogenesis. Mouse embryos at gestational day 8.5 were cultured in vitro for 48 h with 0, 50, 100, 200 or 400 c.f.u. S. aureus L-forms ml−1. At the end of the culture period, the mouse embryos were assessed morphologically for viability, growth and development. Bacteriological and immunohistochemical staining were used to determine the existence of S. aureus L-forms in embryonic tissues. We found that both crown–rump length and head length of mouse embryos exposed to S. aureus L-forms at a concentration of 50 c.f.u. ml−1 were reduced. When the mouse embryos were exposed to 100, 200 or 400 c.f.u. S. aureus L-forms ml−1, the total morphological score, number of somites, dry embryo weight, yolk sac diameter, crown–rump length and head length were significantly lower than those of the control group. With the increased concentration of S. aureus L-forms in the culture medium, there were fewer normally developed embryos and more embryos with abnormalities or retardation in growth. S. aureus L-forms detected by Gram-staining and immunohistochemical detection of antigen were found in the tissues of embryos infected by S. aureus L-forms. These data suggest that S. aureus L-forms exert a direct teratogenic effect on cultured mouse embryos in vitro.

scholarly journals Cell death in rat and mouse embryos exposed to methanol in whole embryo culture

Toxicology ◽  
1995 ◽  
Vol 97 (1-3) ◽  
pp. 159-171 ◽  
Author(s):  
B.D. Abbott ◽  
M. Ebron-mccoy ◽  
J.E. Andrews
Keyword(s):  
Cell Death ◽  
Embryo Culture ◽  
Mouse Embryos ◽  
Whole Embryo Culture

Tissue-specific RNA interference in post-implantation mouse embryos using directional electroporation and whole embryo culture

2004 ◽  
Vol 72 (2-3) ◽  
pp. 92-102 ◽  
Author(s):  
Federico Calegari ◽  
Anne-Marie Marzesco ◽  
Ralf Kittler ◽  
Frank Buchholz ◽  
Wieland B. Huttner
Keyword(s):  
Rna Interference ◽  
Embryo Culture ◽  
Mouse Embryos ◽  
Whole Embryo Culture ◽  
Tissue Specific ◽  
Post Implantation

scholarly journals Postimplantation Whole Embryo Culture Assay for Hamsters: An Alternative to Rat and Mouse

2001 ◽  
Vol 1 ◽  
pp. 227-234 ◽  
Author(s):  
Bogdan Wlodarczyk ◽  
Bogumil Biernacki ◽  
Maria Minta ◽  
Jan Zmudzki
Keyword(s):  
Embryo Culture ◽  
Culture Conditions ◽  
Mouse Embryos ◽  
Total Protein Content ◽  
Image Analysis System ◽  
Whole Embryo Culture ◽  
Analysis System ◽  
Comparison Of The Results ◽  
Rats And Mice

Postimplantation whole embryo culture (WEC) assay for rats and mice has been well established and introduced to many laboratories. Recently WEC technique for rabbits has been developed; however, information on culture of other species is very limited. Knowing the usefulness of hamsters in classical embryotoxicology, we reasoned that hamster WEC could be an alternative model for the most frequently used rat and mouse WEC. Previously we have optimized culture conditions for postimplantation hamster embryos. The aim of this study was to test the susceptibility of hamster embryos cultured in vitro to embryotoxic compounds and to compare our results with those reported by others on rat or mouse embryo culture. For that purpose we choose three known embryotoxic compounds�valproic acid, cadmium chloride, and diethylstilbestrol�and tested them using a postimplantation hamster whole embryo culture assay. Hamster embryos were cultured from 7.5 days gestation for 24 h in a medium consisting of 35% hamster serum and 65% synthetic culture medium (Iscove�s or McCoy 5A). At the end of the culture period, the embryos were examined morphologically, measured with the aid of a computer image analysis system, and total protein content was assessed. All three compounds exhibited dose-related embryotoxic and teratogenic effects in hamster embryos. The malformations observed were similar to those reported on rat and mouse embryos. Comparison of the results with data reported by other authors indicates that hamster embryos cultured in vitromight be more susceptible to embryotoxic stimuli than rat and mouse embryos.

Whole-embryo culture of E5.5 mouse embryos: Development to the gastrulation stage

genesis ◽  
2003 ◽  
Vol 37 (1) ◽  
pp. 38-43 ◽  
Author(s):  
Shigeto Miura ◽  
Yuji Mishina
Keyword(s):  
Embryo Culture ◽  
Mouse Embryos ◽  
Whole Embryo Culture

Whole embryo culture of presomitic mouse embryos

1995 ◽  
Vol 9 (5) ◽  
pp. 671-675 ◽  
Author(s):  
G. Van Maele-fabry ◽  
F. Gofflot ◽  
J.J. Picard
Keyword(s):  
Embryo Culture ◽  
Mouse Embryos ◽  
Whole Embryo Culture

Biosynthesis of DNA, RNA and proteins by mouse embryos cultured in the presence of a teratogenic dose of chlorambucil

Development ◽  
1976 ◽  
Vol 36 (2) ◽  
pp. 273-281
Author(s):  
T. W. Sadler ◽  
D. M. Kochhar
Keyword(s):  
Protein Synthesis ◽  
Dna Synthesis ◽  
Embryo Culture ◽  
Culture Media ◽  
Placental Tissue ◽  
Mouse Embryos ◽  
Whole Embryo Culture ◽  
Rna And Protein Synthesis ◽  
Visceral Yolk Sac ◽  
Reichert's Membrane

The effect of chlorambucil on the rates of DNA, RNA, and protein synthesis in mouse embryos was investigated using a system of whole embryo culture. Embryos were isolated on the 11th day of gestation (33 ± 3 somites) and grown in culture media for periods of 4–8 h. Reichert's membrane and most of the placental tissue was removed leaving only the amnion and visceral yolk-sac surrounding the embryo. In the presence of teratogenic doses of chlorabucil (15 μg/ml) the rate of DNA synthesis was significantly decreased at 4 and 8 h. RNA and protein synthesis were not inhibited at either of these times. A trend toward decreasing rates of protein synthesis at some time beyond 8 h was noted, but not tested.

The effect of sevoflurane on developing A/J strain mouse embryos using a whole-embryo culture system—the incidence of cleft lip in culture embryos

2013 ◽  
Vol 50 (3) ◽  
pp. 237-242 ◽  
Author(s):  
Morimasa Yamada ◽  
Naoki Yamamoto ◽  
Saori Ohgami ◽  
Mayuko Kanazawa ◽  
Jun Harada ◽  
...  
Keyword(s):  
Embryo Culture ◽  
Culture System ◽  
Cleft Lip ◽  
Mouse Embryos ◽  
Whole Embryo Culture ◽  
Strain Mouse

A study of vehicles for dosing rodent whole embryo culture with non aqueous soluble compounds

2004 ◽  
Vol 18 (3) ◽  
pp. 391-398 ◽  
Author(s):  
Karen A Augustine-Rauch ◽  
Qin Zhang ◽  
Mark Kleinman ◽  
Richard Lawton ◽  
Michael J Welsh
Keyword(s):  
Embryo Culture ◽  
Whole Embryo Culture

Flusilazole induces spatio-temporal expression patterns of retinoic acid-, differentiation- and sterol biosynthesis-related genes in the rat Whole Embryo Culture

2016 ◽  
Vol 64 ◽  
pp. 77-85 ◽  
Author(s):  
Myrto Dimopoulou ◽  
Aart Verhoef ◽  
Bennard van Ravenzwaay ◽  
Ivonne M.C.M. Rietjens ◽  
Aldert H. Piersma
Keyword(s):  
Retinoic Acid ◽  
Embryo Culture ◽  
Expression Patterns ◽  
Sterol Biosynthesis ◽  
Temporal Expression ◽  
Whole Embryo Culture ◽  
Spatio Temporal
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