scholarly journals Isolation and Characterization of a 14.5-kDa Trichloroacetic-Acid-Soluble Translational Inhibitor Protein from Human Monocytes that is Upregulated Upon Cellular Differentiation

1996 ◽  
Vol 242 (2) ◽  
pp. 339-351 ◽  
Author(s):  
Gerno Schmiedeknecht ◽  
Claus Kerkhoff ◽  
Evelyn Orso ◽  
Josef Stohr ◽  
Charalampos Aslanidis ◽  
...  
Keyword(s):  
Trichloroacetic Acid ◽  
Cellular Differentiation ◽  
Human Monocytes ◽  
Inhibitor Protein ◽  
Isolation And Characterization ◽  
Translational Inhibitor

scholarly journals Platelet-Derived Extracellular Vesicles for Regenerative Medicine

2021 ◽  
Author(s):  
Miquel Antich-Rosselló ◽  
Maria Antònia Forteza-Genestra ◽  
Marta Monjo ◽  
Joana Maria Ramis
Keyword(s):  
Wound Healing ◽  
Regenerative Medicine ◽  
Extracellular Vesicles ◽  
Cellular Differentiation ◽  
Neural Regeneration ◽  
Isolation And Characterization ◽  
Biomedical Field ◽  
New Treatments ◽  
Different Sources

Extracellular vesicles (EVs) present a great potential for the development of new treatments in the biomedical field. To be used as therapeutics, many different sources have been used for EVs obtention, while only few studies have addressed the use of platelet derived EVs (pEVs). In fact, pEVs have been shown to intervene in different healing responses, thus some studies have evaluated their regenerative capability in wound healing or hemorrhagic shock. Even more, pEVs have proven to induce cellular differentiation, enhancing musculoskeletal or neural regeneration. However, the obtention and characterization of pEVs is widely heterogeneous and differs from the recommendations of the International Society for Extracellular Vesicles. Therefore, in this review, we aim to present the main advances in the therapeutical use of pEVs in the regenerative medicine field while highlighting the isolation and characterization steps followed. The main goal of this review is to portray the studies performed in order to enhance the translation of the pEVs research into feasible therapeutical applications.

scholarly journals Isolation and Characterization of Dominant Mutations in the Bacillus subtilis Stressosome Components RsbR and RsbS

2006 ◽  
Vol 189 (5) ◽  
pp. 1531-1541 ◽  
Author(s):  
Adam Reeves ◽  
W. G. Haldenwang
Keyword(s):  
Bacillus Subtilis ◽  
Inhibitor Protein ◽  
Wild Type ◽  
Homologous Proteins ◽  
Stress Induction ◽  
General Stress Response ◽  
Isolation And Characterization ◽  
Activity State ◽  
Downstream Processes

ABSTRACT The general stress response of Bacillus subtilis is controlled by the activity state of the σB transcription factor. Physical stress is communicated to σB via a large-molecular-mass (>106-Da) structure (the stressosome) formed by one or more members of a family of homologous proteins (RsbR, YkoB, YojH, YqhA). The positive regulator (RsbT) of the σB stress induction pathway is incorporated into the complex bound to an inhibitor protein (RsbS). Exposure to stress empowers an RsbT-dependent phosphorylation of RsbR and RsbS, with the subsequent release of RsbT to activate downstream processes. The mechanism by which stress initiates these reactions is unknown. In an attempt to identify changes in stressosome components that could lead to σB activation, a DNA segment encoding these proteins was mutagenized and placed into B. subtilis to create a merodiploid strain for these genes. Eight mutations that allowed heightened σB activity in the presence of their wild-type counterparts were isolated. Two of the mutations are missense changes in rsbR, and six are amino acid changes in rsbS. Additional experiments suggested that both of the rsbR mutations and three of the rsbS mutations likely enhance σB activity by elevating the level of RsbS phosphorylation. All of the mutations were found to be dominant over wild-type alleles only when they are cotranscribed within an rsbR rsbS rsbT operon. The data suggest that changes in RsbR can initiate the downstream events that lead to σB activation and that RsbR, RsbS, and RsbT likely interact with each other concomitantly with their synthesis.

scholarly journals Platelet-Derived Extracellular Vesicles for Regenerative Medicine

2021 ◽  
Vol 22 (16) ◽  
pp. 8580
Author(s):  
Miquel Antich-Rosselló ◽  
Maria Antònia Forteza-Genestra ◽  
Marta Monjo ◽  
Joana M. Ramis
Keyword(s):  
Wound Healing ◽  
Regenerative Medicine ◽  
Extracellular Vesicles ◽  
Cellular Differentiation ◽  
Neural Regeneration ◽  
Isolation And Characterization ◽  
Biomedical Field ◽  
New Treatments ◽  
Different Sources

Extracellular vesicles (EVs) present a great potential for the development of new treatments in the biomedical field. To be used as therapeutics, many different sources have been used for EVs obtention, while only a few studies have addressed the use of platelet-derived EVs (pEVs). In fact, pEVs have been shown to intervene in different healing responses, thus some studies have evaluated their regenerative capability in wound healing or hemorrhagic shock. Even more, pEVs have proven to induce cellular differentiation, enhancing musculoskeletal or neural regeneration. However, the obtention and characterization of pEVs is widely heterogeneous and differs from the recommendations of the International Society for Extracellular Vesicles. Therefore, in this review, we aim to present the main advances in the therapeutical use of pEVs in the regenerative medicine field while highlighting the isolation and characterization steps followed. The main goal of this review is to portray the studies performed in order to enhance the translation of the pEVs research into feasible therapeutical applications.

The isolation and characterization of 18 equine microsatellite loci, TKY272–TKY289

2000 ◽  
Vol 31 (2) ◽  
pp. 149-149 ◽  
Author(s):  
T Tozaki ◽  
H Kakoi ◽  
S Mashima ◽  
K Hirota ◽  
T Hasegawa ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Isolation And Characterization
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