scholarly journals Platelet-Derived Extracellular Vesicles for Regenerative Medicine

2021 ◽  
Author(s):  
Miquel Antich-Rosselló ◽  
Maria Antònia Forteza-Genestra ◽  
Marta Monjo ◽  
Joana Maria Ramis
Keyword(s):  
Wound Healing ◽  
Regenerative Medicine ◽  
Extracellular Vesicles ◽  
Cellular Differentiation ◽  
Neural Regeneration ◽  
Isolation And Characterization ◽  
Biomedical Field ◽  
New Treatments ◽  
Different Sources

Extracellular vesicles (EVs) present a great potential for the development of new treatments in the biomedical field. To be used as therapeutics, many different sources have been used for EVs obtention, while only few studies have addressed the use of platelet derived EVs (pEVs). In fact, pEVs have been shown to intervene in different healing responses, thus some studies have evaluated their regenerative capability in wound healing or hemorrhagic shock. Even more, pEVs have proven to induce cellular differentiation, enhancing musculoskeletal or neural regeneration. However, the obtention and characterization of pEVs is widely heterogeneous and differs from the recommendations of the International Society for Extracellular Vesicles. Therefore, in this review, we aim to present the main advances in the therapeutical use of pEVs in the regenerative medicine field while highlighting the isolation and characterization steps followed. The main goal of this review is to portray the studies performed in order to enhance the translation of the pEVs research into feasible therapeutical applications.

scholarly journals Platelet-Derived Extracellular Vesicles for Regenerative Medicine

2021 ◽  
Vol 22 (16) ◽  
pp. 8580
Author(s):  
Miquel Antich-Rosselló ◽  
Maria Antònia Forteza-Genestra ◽  
Marta Monjo ◽  
Joana M. Ramis
Keyword(s):  
Wound Healing ◽  
Regenerative Medicine ◽  
Extracellular Vesicles ◽  
Cellular Differentiation ◽  
Neural Regeneration ◽  
Isolation And Characterization ◽  
Biomedical Field ◽  
New Treatments ◽  
Different Sources

Extracellular vesicles (EVs) present a great potential for the development of new treatments in the biomedical field. To be used as therapeutics, many different sources have been used for EVs obtention, while only a few studies have addressed the use of platelet-derived EVs (pEVs). In fact, pEVs have been shown to intervene in different healing responses, thus some studies have evaluated their regenerative capability in wound healing or hemorrhagic shock. Even more, pEVs have proven to induce cellular differentiation, enhancing musculoskeletal or neural regeneration. However, the obtention and characterization of pEVs is widely heterogeneous and differs from the recommendations of the International Society for Extracellular Vesicles. Therefore, in this review, we aim to present the main advances in the therapeutical use of pEVs in the regenerative medicine field while highlighting the isolation and characterization steps followed. The main goal of this review is to portray the studies performed in order to enhance the translation of the pEVs research into feasible therapeutical applications.

scholarly journals 109 Isolation and characterization of myometrium and decidual cell-derived extracellular vesicles

2021 ◽  
Vol 224 (2) ◽  
pp. S75-S76
Author(s):  
Megan Shepherd ◽  
Enkhtuya Radnaa ◽  
Rheanna Urrabaz-Garza ◽  
Talar Kechichian ◽  
Ourlad Alzeus G. Tantengco ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Decidual Cell ◽  
Isolation And Characterization

scholarly journals Emerging techniques in the isolation and characterization of extracellular vesicles and their roles in cancer diagnostics and prognostics

The Analyst ◽  
2016 ◽  
Vol 141 (2) ◽  
pp. 371-381 ◽  
Author(s):  
Vijaya Sunkara ◽  
Hyun-Kyung Woo ◽  
Yoon-Kyoung Cho
Keyword(s):  
Extracellular Vesicles ◽  
Cancer Diagnostics ◽  
Clinical Implementation ◽  
Characterization Methods ◽  
Cancer Management ◽  
Isolation And Characterization

We present an overview of current isolation, detection, and characterization methods of extracellular vesicles and their applications and limitations as a potential emerging biomarker in cancer management and their clinical implementation.

Isolation and Characterization of Extracellular Vesicles in Stem Cell-Related Studies

2017 ◽  
pp. 205-223
Author(s):  
Zezhou Zhao ◽  
Dillon C. Muth ◽  
Vasiliki Mahairaki ◽  
Linzhao Cheng ◽  
Kenneth W. Witwer
Keyword(s):  
Stem Cell ◽  
Extracellular Vesicles ◽  
Isolation And Characterization

scholarly journals Correction to: Isolation and Characterization of Extracellular Vesicles from Keratinocyte Cultures

2019 ◽  
pp. 293-293
Author(s):  
Sebastian Sjöqvist ◽  
Aya Imafuku ◽  
Dhanu Gupta ◽  
Samir EL Andaloussi
Keyword(s):  
Extracellular Vesicles ◽  
Isolation And Characterization

scholarly journals A Simplified Method for the Aspiration of Bone Marrow from Patients Undergoing Hip and Knee Joint Replacement for Isolating Mesenchymal Stem Cells and In Vitro Chondrogenesis

2016 ◽  
Vol 2016 ◽  
pp. 1-18 ◽  
Author(s):  
Subhash C. Juneja ◽  
Sowmya Viswanathan ◽  
Milan Ganguly ◽  
Christian Veillette
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Regenerative Medicine ◽  
Isolation And Characterization ◽  
Standard Operating ◽  
Total Knee ◽  
Detailed Protocol

The procedure for aspiration of bone marrow from the femur of patients undergoing total knee arthroplasty (TKA) or total hip arthroplasty (THA) may vary from an OR (operating room) to OR based on the surgeon’s skill and may lead to varied extent of clotting of the marrow and this, in turn, presents difficulty in the isolation of mesenchymal stem cells (MSCs) from such clotted bone marrow. We present a simple detailed protocol for aspirating bone marrow from such patients, isolation, and characterization of MSCs from the aspirated bone marrow specimens and show that the bone marrow presented no clotting or exhibited minimal clotting. This represents an economical source and convenient source of MSCs from bone marrow for use in regenerative medicine. Also, we presented the detailed protocol and showed that the MSCs derived from such bone marrow specimens exhibited MSCs characteristics and generated micromass cartilages, the recipe for regenerative medicine for osteoarthritis. The protocols we presented can be used as standard operating procedures (SOPs) by researchers and clinicians.

scholarly journals Isolation and characterization of platelet-derived extracellular vesicles

2014 ◽  
Vol 3 (1) ◽  
pp. 24692 ◽  
Author(s):  
Maria T. Aatonen ◽  
Tiina Öhman ◽  
Tuula A. Nyman ◽  
Saara Laitinen ◽  
Mikaela Grönholm ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Isolation And Characterization

An Emerging Fluorescence-Based Technique for Quantification and Protein Profiling of Extracellular Vesicles

2020 ◽  
pp. 247263032097045
Author(s):  
Mehdi Dehghani ◽  
Rebecca K. Montange ◽  
Michael W. Olszowy ◽  
David Pollard
Keyword(s):  
Flow Cytometry ◽  
Extracellular Vesicles ◽  
Protein Profiling ◽  
Submicron Particles ◽  
Protein Markers ◽  
Bulk Analysis ◽  
Precise Quantification ◽  
Therapeutic Tools ◽  
Different Sources

Robust and well-established techniques for the quantification and characterization of extracellular vesicles (EVs) are a crucial need for the utilization of EVs as potential diagnostic and therapeutic tools. Current bulk analysis techniques such as proteomics and Western blot suffer from low resolution in the detection of small changes in target marker expression levels, exemplified by the heterogeneity of EVs. Microscopy-based techniques can provide valuable information from individual EVs; however, they are time-consuming and statistically less powerful than other techniques. Flow cytometry has been successfully employed for the quantification and characterization of individual EVs within larger populations. However, traditional flow cytometry is not highly suited for the examination of smaller, submicron particles. Here we demonstrate the accurate and precise quantification of nanoparticles such as EVs using the Virus Counter 3100 (VC3100) platform, a fluorescence-based technique that uses the principles of flow cytometry with critical enhancements to enable the effective detection of smaller particles. This approach can detect nanoparticles precisely with no evidence of inaccurate concentration measurement from masking effects associated with traditional nanoparticle tracking analysis (NTA). Fluorescently labeled EVs from different sources were successfully quantified using the VC3100 without a postlabeling washing step. Moreover, protein profiling and characterization of individual EVs were achieved and have been shown to determine the expression level of target protein markers.

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