Modes of Self-Pollination and Absence of Cryptic Self-Incompatibility inDrosophyllum lusitanicum(Droseraceae)

Botanica Acta ◽  
1998 ◽  
Vol 111 (6) ◽  
pp. 474-480 ◽  
Author(s):  
A. Ortega-Olivencia ◽  
J. A. López Paredes ◽  
T. Rodríguez-Riaño ◽  
J. A. Devesa
Keyword(s):  
Self Incompatibility ◽  
Self Pollination

scholarly journals Cytological Observation and Transcriptome Comparative Analysis of Self-Pollination and Cross-Pollination in Dendrobium Officinale

Genes ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 432
Author(s):  
Yaling Chen ◽  
Benchang Hu ◽  
Fantao Zhang ◽  
Xiangdong Luo ◽  
Jiankun Xie
Keyword(s):  
Pollen Tubes ◽  
The Self ◽  
Dendrobium Officinale ◽  
Self Incompatibility ◽  
Related Gene ◽  
Cytological Observation ◽  
Lectin Receptor ◽  
Cross Pollination ◽  
Self Pollination ◽  
Medicinal Value

Dendrobium officinale is a rare and traditional medicinal plant with high pharmacological and nutritional value. The self-incompatibility mechanism of D. officinale reproductive isolation was formed in the long-term evolution process, but intraspecific hybridization of different germplasm resources leads to a large gap in the yield, quality, and medicinal value of D. officinale. To investigate the biological mechanism of self-incompatibility in D. officinale, cytological observation and the transcriptome analysis was carried out on the samples of self-pollination and cross-pollination in D. officinale. Results for self-pollination showed that the pollen tubes could grow in the style at 2 h, but most of pollen tubes stopped growing at 4 h, while a large number of cross-pollinated pollen tubes grew along the placental space to the base of ovary, indicating that the self-incompatibility of D. officinale may be gametophyte self-incompatibility. A total of 63.41 G basesum of D. officinale style samples from non-pollinated, self-pollination, and cross-pollination by RNA-seq were obtained, and a total of 1944, 1758, and 475 differentially expressed genes (DEGs) in the comparison of CK (non-pollinated) vs. HF (cross-pollination sample), CK vs. SF (self-pollination sample) and SF vs. HF were identified, respectively. Forty-one candidate genes related to self-incompatibility were found by function annotation of DEGs, including 6 Ca2+ signal genes, 4 armed repeat containing (ARC) related genes, 11 S-locus receptor kinase (SRK) related genes, 2 Exo70 family genes, 9 ubiquitin related genes, 1 fatty acid related gene, 6 amino acid-related genes, 1 pollen-specific leucine-rich repeat extensin-like protein (LRX) related gene and 1 lectin receptor-like kinases (RLKs) related gene, showed that self-incompatibility mechanism of D. officinale involves the interaction of multiple genes and pathways. The results can provide a basis for the study of the self-incompatibility mechanism of D. officinale, and provide ideas for the preservation and utilization of high-quality resources of D. officinale.

Pollen flow in distylous populations of Amsinckia (Boraginaceae)

1976 ◽  
Vol 54 (22) ◽  
pp. 2530-2535 ◽  
Author(s):  
Fred R. Ganders
Keyword(s):  
Pollen Flow ◽  
Progeny Testing ◽  
Self Incompatibility ◽  
Disassortative Mating ◽  
Pollen Loads ◽  
Self Pollination ◽  
Incompatibility System ◽  
Total Pollen

Stigmatic pollen loads were analyzed from naturally pollinated pin and thrum form flowers of Amsinckia douglasiana and A. vernicosa var. furcata. Pin stigmas captured more total pollen than thrum stigmas. Pins experienced either net self-pollination or random pollination. Thrum stigmas experienced significant disassortative pollination. Comparing pollen loads from intact and emasculated thrum flowers of A. douglasiana indicated that self-pollination and geitonogamy were relatively unimportant in the pollination of the thrum form. The level of disassortative pollination of A. vernicosa var. furcata does not appear to be high enough to account for the level of disassortative mating observed by progeny testing, suggesting that this species may possess an incomplete stylar self-incompatibility system such as has been reported in A. grandiflora.

Hand-Tripped Flowers Promote Seed Production in Arachis lignosa, a Wild Peanut1

1990 ◽  
Vol 17 (1) ◽  
pp. 22-24 ◽  
Author(s):  
Donald J. Banks
Keyword(s):  
Seed Production ◽  
Growth Chamber ◽  
Self Incompatibility ◽  
Self Pollination ◽  
Reproductive Sterility ◽  
High Degree

Abstract In trials conducted in the greenhouse, growth chamber, and outdoors, no pegs or pods were produced when A. lignosa (Chod. et Hassl.) Krap. et Greg. nom. nud. plants were allowed to pollinate naturally. However, hand tripping flowers, especially in the greenhouse, resulted in significant increases in pod production. The results suggest that the high degree of reproductive sterility usually noted for A. lignosa in culture is due to pollination failure rather than to physiological self incompatibility. The somewhat truncated shape of the stigma and its elevated position relative to the anthers probably restricts natural self-pollination without the aid of pollinating vectors such as bees.

Electrophoretic evidence supporting self-incompatibility in Lotus corniculatus

1980 ◽  
Vol 58 (6) ◽  
pp. 712-716 ◽  
Author(s):  
Shirley Dobrofsky ◽  
W. F. Grant
Keyword(s):  
Gel Electrophoresis ◽  
Seed Set ◽  
Polyacrylamide Gel Electrophoresis ◽  
Lotus Corniculatus ◽  
Polyacrylamide Gel ◽  
Self Incompatibility ◽  
Banding Patterns ◽  
Protein Subunits ◽  
Self Pollination

Self-incompatibility, a prefertilization event, and self-sterility, a postfertilization event, have both been suggested as causes for differences in seed set between cross- and self-pollinated florets in Lotus corniculatus L. Ovary protein subunits of selfed, crossed, and unpollinated florets of L. corniculatus cv. Mirabel were studied using polyacrylamide gel electrophoresis. Banding patterns differed for all three conditions. Ovary protein differences were found prior to the time fertilization is known to occur, thereby providing evidence that self-incompatibility is at least partially responsible for the reduced seed set after self-pollination.

scholarly journals Cultivar and Developmental Differences in S-protein Concentration and Self-incompatibility in the Japanese Pear

HortScience ◽  
2000 ◽  
Vol 35 (5) ◽  
pp. 917-920 ◽  
Author(s):  
Shao-Ling Zhang ◽  
Shin Hiratsuka
Keyword(s):  
Pollen Tubes ◽  
Tube Growth ◽  
Cultivar Differences ◽  
Japanese Pear ◽  
Pyrus Pyrifolia ◽  
Self Incompatibility ◽  
S Protein ◽  
Self Pollination ◽  
Rate Of Increase ◽  
S Proteins

Cultivars of the Japanese pear [Pyrus pyrifolia (Burm.) Nakai] have variable degrees of self-incompatibility (SI) and can be classified into at least three groups: strong, intermediate, or weak SI; as shown by the extent of self-pollen tube growth in the style, and the percentage of fruit set following self-pollination. Following self-pollination, the elongation of pollen tubes in the detached styles of `Kosui' and `Kikusui' became increasingly suppressed from 4 days before anthesis (–4 DAA) to 2 days after anthesis (2 DAA). Tube growth of `Kosui' was more suppressed than that of `Kikusui' during this period. In `Osa-Nijisseiki', however, the rate of tube growth did not vary with stage of stylar development, from –8 to 2 DAA. Pollen tubes elongated much better after cross-pollination than after self-pollination at all stages tested, and the extent of the elongation increased as the styles matured. The concentration of total S-protein (sum of two S-proteins per buffer-soluble protein) increased with stylar development, but the rate of increase varied with the cultivar. The rate was significantly greater in the strongly self-incompatible `Kosui' than in the moderately self-incompatible `Kikusui', and was slowest in the weakly self-incompatible `Osa-Nijisseiki' at all developmental stages. During stylar maturation, the concentration of S4-protein, which is common in all cultivars, was highest in `Kosui', followed by `Kikusui' and `Osa-Nijisseiki'. Thus, the cultivar differences in SI expression in the Japanese pear are determined about –4 DAA and appear to be regulated, in part, by the concentration of S-proteins produced in the style.

scholarly journals Self-incompatibility and Self-fruitfulness in Pear cv. Agua de Aranjuez

2007 ◽  
Vol 132 (2) ◽  
pp. 166-171 ◽  
Author(s):  
Javier Sanzol ◽  
Maria Herrero
Keyword(s):  
Pollen Tube ◽  
Fruit Set ◽  
Parthenocarpic Fruit ◽  
Self Incompatibility ◽  
Variable Response ◽  
Self Pollination ◽  
Fruit Setting ◽  
Self Fertilization ◽  
Growing Conditions ◽  
Performance Results

Most pear (Pyrus communis L.) cultivars are impaired to set fruit under self-pollination, because self-fertilization is prevented by a gametophytic self-incompatibility system. However, accumulated information in this species shows that often for a same cultivar, after self-pollination, a variable response in fruit set can be obtained in different years or growing conditions. In this work, we characterize self-incompatibility and self-fruitfulness in ‘Agua de Aranjuez’, the main Spanish pear cultivar, which also shows a variable response to self-pollination. Two years with a different fruit setting response after self-pollination, one with no fruit set and the other with a moderate fruit set, were compared for parthenocarpic fruit development and for pollen tube performance. Results show that in both years, this cultivar behaves as self-incompatible with absence of parthenocarpy. In selfed flowers, most pollen tubes are arrested in the upper half of the style, although in a small proportion of the styles, a pollen tube can reach the base of the style and eventually effect fertilization. Self-fertilization, although occurring at a low level, can explain the fruit set levels obtained under self-pollination given that flowers with just one fertilized ovule are able to set fruit. This behavior could explain confusing results about self-fruitfulness in ‘Agua de Aranjuez’ and other pear cultivars.

scholarly journals Reproductive Success after Self-pollination and Cross-pollination of Olive Cultivars in Croatia

HortScience ◽  
2011 ◽  
Vol 46 (2) ◽  
pp. 186-191 ◽  
Author(s):  
Gabriela Vuletin Selak ◽  
Slavko Perica ◽  
Smiljana Goreta Ban ◽  
Mira Radunic ◽  
Milan Poljak
Keyword(s):  
Reproductive Behavior ◽  
Fruit Set ◽  
Positive Response ◽  
Flowering Period ◽  
Self Incompatibility ◽  
Cross Pollination ◽  
Self Pollination ◽  
Cross Compatibility ◽  
Olive Cultivars ◽  
Simultaneous Flowering

Olive orchard productivity largely depends on the choice of planted cultivars and their pollination needs. Orchard designs in Croatia are changing because a number of valuable foreign olive cultivars, mostly Italian, have been introduced in this region in the last 30 years. The compatibility relationships of introduced cultivars with autochthonous cultivars are unknown. With the objective of studying reproductive behavior of the most important Croatian cultivars (Drobnica, Lastovka, Levantinka, and Oblica) and their cross-pollination to recently introduced Italian cultivars Leccino and Pendolino, initial and final fruit set in self-pollination versus cross-pollination and free pollination were compared during three flowering seasons. Experiments were conducted in three different orchards (Kastela, Mravince, and Brac) to identify the effect of the environment on reproductive behavior of olive cultivars. The differences of fruit set in five olive cultivars after tested pollination treatments appeared at the time of initial fruit set. Increased final fruit set under cross-pollinations was observed when compared with self-pollination for all olive cultivars in all experimental orchards. In the Mravince orchard, a positive response to cross-pollination was consistent, and fruit set increased under cross-pollination in all cultivars and years with the exception of ‘Levantinka’ in which no significant differences were noticed between self-pollination treatment and cross-pollination treatments in 2005. Variable self-fertility behavior from season to season was found for tested cultivars. A self-incompatibility index (ISI) higher than 0.1 was recorded for ‘Levantinka’ in all experimental years and, therefore, classified it as a partially self-incompatible cultivar. Self-incompatibility response was observed for ‘Lastovka’. The positive response to cross-pollination over self-pollination only in some experimental years classified ‘Drobnica’, ‘Leccino’, and ‘Oblica’ as partially self-incompatible. Results obtained from this study indicated that pollination efficiency is strictly combination-specific. The Italian cultivar, Leccino, was a successful pollen acceptor and pollenizer of most Croatian cultivars. Reciprocal high success in cross-pollination was recorded for ‘Levantinka’ and ‘Oblica’. In the Mravince orchard, ‘Lastovka’, ‘Leccino’, and ‘Oblica’ were efficient pollenizers of ‘Levantinka’ where the simultaneous flowering period was in accordance with their cross-compatibility. ‘Levantinka’ was a good pollenizer for ‘Lastovka’ in the Mravince orchard, and both cultivars entered into the flowering period earlier than other studied cultivars, which was not the case in the other two orchards. The variations in flowering timing among orchards were a consequence of differences in environmental conditions. According to the high fruit sets recorded in ‘Oblica’ after pollination with ‘Leccino’ or ‘Levantinka’, an increase in tree productivity of the acceptor cultivar is expected in the presence of selected pollenizers in all olive-growing regions.

scholarly journals Transcriptomic Analysis Reveals a Comprehensive Calcium- and Phytohormone-Dominated Signaling Response in Leymus chinensis Self-Incompatibility

2019 ◽  
Vol 20 (9) ◽  
pp. 2356 ◽  
Author(s):  
Shuangyan Chen ◽  
Junting Jia ◽  
Liqin Cheng ◽  
Pincang Zhao ◽  
Dongmei Qi ◽  
...  
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Time Course ◽  
Molecular Mechanisms ◽  
Tube Growth ◽  
Leymus Chinensis ◽  
Self Incompatibility ◽  
Cross Pollination ◽  
Grass Family ◽  
Self Pollination

Sheepgrass (Leymus chinensis (Trin.) Tzvel.) is an economically and ecologically important forage in the grass family. Self-incompatibility (SI) limits its seed production due to the low seed-setting rate after self-pollination. However, investigations into the molecular mechanisms of sheepgrass SI are lacking. Therefore, microscopic observation of pollen germination and pollen tube growth, as well as transcriptomic analyses of pistils after self- and cross-pollination, were performed. The results indicated that pollen tube growth was rapidly inhibited from 10 to 30 min after self-pollination and subsequently stopped but preceded normally after cross-pollination. Time course comparative transcriptomics revealed different transcriptome dynamics between self- and cross-pollination. A pool of SI-related signaling genes and pathways was generated, including genes related to calcium (Ca2+) signaling, protein phosphorylation, plant hormone, reactive oxygen species (ROS), nitric oxide (NO), cytoskeleton, and programmed cell death (PCD). A putative SI response molecular model in sheepgrass was presented. The model shows that SI may trigger a comprehensive calcium- and phytohormone-dominated signaling cascade and activate PCD, which may explain the rapid inhibition of self-pollen tube growth as observed by cytological analyses. These results provided new insight into the molecular mechanisms of sheepgrass (grass family) SI.

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