Morphoquantitative Aspects of NADH-Diaphorase Myenteric Neurons in the Ileum of Diabetic Rats Treated with Acetyl-l-Carnitine

2006 ◽  
Vol 35 (1) ◽  
pp. 13-18 ◽  
Author(s):  
A. M. P. Alves ◽  
E. P. B. Alves ◽  
C. E. P. T. Fregonesi ◽  
M. A. Defani ◽  
S. R. Stabille ◽  
...  
Keyword(s):  
Diabetic Rats ◽  
Myenteric Neurons ◽  
Nadh Diaphorase

scholarly journals Effects of the ascorbic acid supplementation on NADH-diaphorase myenteric neurons in the duodenum of diabetic rats

BIOCELL ◽  
2006 ◽  
Vol 30 (2) ◽  
pp. 295-300 ◽  
Author(s):  
MARLI APARECIDA DOS SANTOS PEREIRA ◽  
MARIA CLA贒IA BAGATIN ◽  
JACQUELINE NELISIS ZANONI
Keyword(s):  
Ascorbic Acid ◽  
Diabetic Rats ◽  
Acid Supplementation ◽  
Myenteric Neurons ◽  
Nadh Diaphorase ◽  
Ascorbic Acid Supplementation

scholarly journals Effect of the ascorbic acid treatment on the NADHd-positive myenteric neurons of diabetic rats proximal colon

2007 ◽  
Vol 50 (1) ◽  
pp. 31-38 ◽  
Author(s):  
Jacqueline Nelisis Zanoni ◽  
Renata Virginia Fernandes Pereira ◽  
Priscila de Freitas
Keyword(s):  
Ascorbic Acid ◽  
Acid Treatment ◽  
Neuroprotective Effect ◽  
Proximal Colon ◽  
Diabetic Rats ◽  
Daily Treatment ◽  
Body Area ◽  
Neuronal Density ◽  
Myenteric Neurons ◽  
Intestinal Segments

The aim of this work was to study the effect of the ascorbic acid on the myenteric neurons of diabetic rats proximal colon. Fifteen rats (90 days old) were divided into three groups: control, untreated diabetic and treated diabetic with ascorbic acid (DA). After 120 days of daily treatment with ascorbic acid, the intestinal segments were submitted to the NADH-diaphorase (NADHd) histochemistry technique to expose the myenteric neurons. The group DA showed a higher neuronal density (33.4 %) when compared to the untreated diabetic animals (p < 0.05). Cellular body area of neurons was significantly larger in group DA (17.3 %) when compared to the untreated diabetics (p < 0.05). It could be concluded that the ascorbic acid promoted a neuroprotective effect on the NADHd myenteric neurons of the proximal colon of diabetic rats.

scholarly journals Effects of L-glutamine supplementation on the myenteric neurons from the duodenum and cecum of diabetic rats

2011 ◽  
Vol 48 (1) ◽  
pp. 66-71 ◽  
Author(s):  
Jacqueline Nelisis Zanoni ◽  
Eleandro Aparecido Tronchini ◽  
Sheila Alves Moure ◽  
Ivan Domicio da Silva Souza
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Neuroprotective Effect ◽  
Natural Aging ◽  
Neurological Complications ◽  
Diabetic Rats ◽  
Glutamine Supplementation ◽  
Complications Of Diabetes ◽  
Myenteric Neurons ◽  
Cellular Antioxidants

CONTEXT: Peripheral neuropathy is one of the chronic complications of diabetes mellitus and is directly related to gastrointestinal consequences of the disease. Myenteric neurons are affected in some pathological conditions such as diabetic neuropathy. The imbalance between cellular antioxidants and free radicals, leading to an increase in oxidative stress, is considered one of the main factors responsible for neuronal damages in diabetes. Drugs that reduce the oxidative stress may play a significant role in the treatment of neurological complications of diabetes mellitus. OBJECTIVE: To evaluate the effect of L-glutamine supplementation on the myenteric neurons from the cecum and duodenum of Wistar rats with streptozotocin-induced diabetes mellitus. METHODS: The animals were divided in four groups (n = 5): non-treated normoglycemics, normoglycemics treated with L-glutamine, non-treated diabetics and diabetics treated with L-glutamine from the 4th day of diabetes induction on. The amino acid L-glutamine was added to their diet at 1%. Giemsa's technique was employed to stain the myenteric neurons. We determined the cell body area of 500 neurons in each group studied. The quantitative analysis was performed by sampling in an area of 16.6 mm² in the cecum and 3.6 mm² in the duodenum of each animal. RESULTS: After the supplementation with L-glutamine in the duodenum, we observed a preservation of neuronal density in groups normoglycemic and diabetic (P<0.05). We also observed a preservation of the cell bodies area in diabetic animals (group treated with L-glutamine) (P<0.05). In the cecum, that preservation was not evident. CONCLUSION: Supplementation with L-glutamine (1%) promoted a neuroprotective effect on the myenteric neurons from the duodenum of rats, both in terms of natural aging and of diabetes mellitus.

scholarly journals Effects of ascorbic acid supplementation in ileum myenteric neurons of streptozotocin-induced diabetic rats

2009 ◽  
Vol 29 (4) ◽  
pp. 295-302
Author(s):  
Sonia M. Silverio ◽  
Renata de B. Mari ◽  
Naianne K. Clebis ◽  
Juliana R. Scoz ◽  
Ricardo de M. Germano ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Neuroprotective Effect ◽  
Polyol Pathway ◽  
Diabetic Rats ◽  
Animal Group ◽  
Neuronal Density ◽  
Myenteric Neurons ◽  
Metabolic Characteristics ◽  
Profile Area ◽  
Ascorbic Acid Supplementation

The exacerbation of the oxidative stress and of the polyol pathway which impair damage myenteric plexus are metabolic characteristics of diabetes. The ascorbic acid (AA) is an antioxidant and an aldose reductase inhibitor, which may act as neuroprotector. The effects of AA supplementation on the density and cellular body profile area (CP) of myenteric neurons in STZ-induced diabetes in rats were assessed. Four groups with five animals each were formed: normoglycemic (C); diabetic (D); AA-treated diabetic (DS) and AA-treated normoglycemic (CS). Dosagen of 50mg of AA were given, three times a week, for each animal (group DS and CS). Ninety days later and after euthanasia, the ileum was collected and processed for the NADPH-diaphorase technique. There were no differences (P>0.05) in the neuronal density among the groups. The CP area was lower (P<0.05) in the DS and CS groups, with a higher incidence of neurons with a CP area exceeding 200µm² for groups C and D. The AA had no influence on the neuronal density in the ileum but had a neuroprotective effect, preventing the increase in the CP area and allowing a higher number of neurons with a CP area with less than 200µm².

scholarly journals Effect of l-glutamine on myenteric neuron and of the mucous of the ileum of diabetic rats

2013 ◽  
Vol 85 (3) ◽  
pp. 1165-1176 ◽  
Author(s):  
ELEANDRO A. TRONCHINI ◽  
ALINE R. TREVIZAN ◽  
CRISTIANO M. TASHIMA ◽  
PRISCILA DE FREITAS ◽  
ROBERTO B. BAZOTTE ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Goblet Cells ◽  
Diabetic Rats ◽  
Glutamine Supplementation ◽  
Body Area ◽  
Myenteric Neurons ◽  
Induced Changes ◽  
Group D ◽  
Similar Depth ◽  
Diabetes Induction

The objective of this work was to investigate the effect of the L-glutamine supplementation to prevent - diabetes induced changes in myenteric neurons and also to verify the effect on the mucosa of the ileum of Wistar rats. The animals were divided in five groups (n = 5): untreated normoglycaemic (UN), normoglycaemic treated with L-glutamine (NG), untreated diabetics (UD), diabetics treated with L-glutamine, starting on the 4th (DG4) or 45th day following diabetes induction (DG45). The amino acid was added to the diet at 1%. The density and size of neurons, the metaphasic index in the crypt, the height of the villus, the depth of the crypt and the number of globet cells were determined. There was no difference in the neuronal density and in the cellular body area of the myosin-stained myenteric neurons of groups DG4 and DG45 when compared to group D. The metaphase index and the number of goblet cells showed no significant differences when all groups were compared (P > 0.05). The villi height of groups DG4 and DG45 were 45.5% (P < 0.05) and 32.4% (P > 0.05) higher than those in group UD, respectively. The analyzed crypts showed similar depth for all studied groups.

scholarly journals Assessment of NADPH-diaphorase stained myenteric neurons of the jejunum of diabetic rats supplemented with ascorbic acid

2008 ◽  
Vol 28 (2) ◽  
pp. 95-102 ◽  
Author(s):  
Sônia M. Silverio ◽  
Renata de B. Mari ◽  
Naianne K. Clebis ◽  
Juliana R. Scoz ◽  
Ricardo de M. Germano ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Diabetic Neuropathy ◽  
Neuroprotective Effect ◽  
Diabetic Rats ◽  
Nadph Diaphorase ◽  
Myenteric Neurons ◽  
Space Organization ◽  
Male Wistar Rats ◽  
Group D ◽  
Profile Area

The relation between hyperglycemia and diabetic neuropathy has already been demonstrated in some studies. Among the theories proposed for its etiology the oxidative stress stands out. The performance of nitric oxide as a link between the metabolic and vascular neuropathogenic factors that triggers the diabetic neuropathy has already been put forward. This study aimed to assess the quantification and measurements of the cell body profile area (CBPA) of NADPH-diaphorase reactive (NADPH-dp) myenteric neurons of the jejunum of diabetic rats (induced by streptozotocin) supplemented with Ascorbic Acid (AA). These changes in the myenteric neurons seem to be related to the gastrointestinal disturbances observed in diabetes mellitus (DM). Twenty male Wistar rats (Rattus norvegicus) were distributed in 4 groups (n=5): controls (C), control supplemented (CS), diabetic (D), and diabetic suplemented (DS). DM was induced by estreptozotocin (50mg/kg body wt). One week after the induction and confirmation of the DM (glycemia exam), animals of the groups CS and DS received 50mg of AA three times a week by gavage. After 90 days of experiment, the animals were anesthetized with lethal thiopental dose (40mg/kg) and the collected jejunum processed for the histochemistry NADPH-diaphorase technique. Whole-mount preparations were obtained for quantitative and morphometric analysis of the myenteric neurons. A quantity of jejunum neurons in the Group D (96±7.5) was not different (P>0.05) from Group DS (116±8.08), C (92±9.7), and CS (81±5.4), but in Group DS the quantity was higher (P<0.05) than in Group C and CS. The CBPA of neurons from Group D (189.50±2.68µm²) and DS (195.92±3.75µm²) were lower (P<0.05) than from Group C (225.13±4.37µm²) and CS (210.23±3.15µm²). The streptozotocin-induced DM did not change the jejunum-ileum area, the jejunum myenteric plexus space organization and the density of NADPH-dp neurons. The 50g AA-supplementation, three times a week, during 90 days, did not decrease hyperglycemia; however, it had a neuroprotective effect on the myenteric neurons, minimizing the increase on the CBPA of NADPH-dp neurons and increasing the amount of NADPD-dp neurons.

scholarly journals Number and size of myenteric neurons of the duodenum of adult rats with acute diabetes

1999 ◽  
Vol 57 (3B) ◽  
pp. 740-745 ◽  
Author(s):  
MARIA MONTSERRAT D.P. FURLAN ◽  
MARCÍLIO H. DE MIRANDA-NETO ◽  
DÉBORA DE MELLO G. SANT'ANA ◽  
SÔNIA L. MOLINARI
Keyword(s):  
Body Weight ◽  
Experimental Period ◽  
The Other ◽  
Adult Rats ◽  
Myenteric Neurons ◽  
Nadh Diaphorase ◽  
Other Hand ◽  
Acute Diabetes

This study had as its purpose to assess the effects of acute diabetes induced by streptozotocin (35 mg/kg body weight) on the number and size of the myenteric neurons of the duodenum of adult rats considering equally the antimesenteric and intermediate regions of the intestinal circumference. Experimental period extended for a week. Neuronal counts were carried out on the same number of fields of both regions of the duodenal circumference and measurements of neuronal and nuclear areas on equal numbers of cells. Number and size of the myenteric neurons stained with Giemsa were not significantly different between groups. On the other hand, the proportion of NADH-positive neurons increased from 18.54% on the controls to 39.33% on the diabetics. The authors discuss that this increased reactivity probably results from a greater NADH/NAD+ ratio, described in many tissues of diabetic animals, which has consequences on the modulation of the enzymes that use these cofactors and whose activity is detected by the NADH-diaphorase technique.

scholarly journals Regional differences in the number and type of myenteric neurons in the descending colon of rats

2003 ◽  
Vol 61 (2A) ◽  
pp. 220-225 ◽  
Author(s):  
Eduardo José de Almeida Araújo ◽  
Débora de Mello Gonçales Sant'Ana ◽  
Sônia Lucy Molinari ◽  
Marcílio Hubner de Miranda Neto
Keyword(s):  
Light Microscopy ◽  
Myenteric Plexus ◽  
Regional Differences ◽  
Nadph Diaphorase ◽  
Intermediate Region ◽  
Neuronal Density ◽  
Myenteric Neurons ◽  
Nadh Diaphorase ◽  
Descending Colon ◽  
Whole Mounts

The purpose of this study was to analyze the neuronal density of the myenteric plexus of the intermediate and antimesocolic regions of the descending colon of rats. Whole-mounts were stained with three different techniques of neuronal evidenciation. Through counts of the number of neurons in an area of 6.64 mm² under light microscopy, we found 1,271 ± 227.54 neurons with Giemsa in the intermediate region and 1,234 ± 225.92 neurons in the antimesocolic region; with the NADH-diaphorase technique we found 530 ± 92.97 neurons in the intermediate region and 539 ± 146.72 neurons in the antimesocolic region; and through the NADPH-diaphorase histochemistry, we found 417 ± 34.42 neurons in the intermediate region and 547 ± 84.01 neurons in the antimesocolic region. We conclude that there is a variation in the density of NADPH-diaphorase positive neurons in the intestinal circumference; that the NADH-diaphorase positive neuronal subpopulation represented 42.7% of that stained with Giemsa; and that the NADPH-diaphorase positive neurons represented 37.8% of the whole myenteric population.

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