Skin surface lipids and skin and hair coat condition in dogs fed increased total fat diets containing polyunsaturated fatty acids

2009 ◽  
Vol 93 (4) ◽  
pp. 505-511 ◽  
Author(s):  
N. A. Kirby ◽  
S. L. Hester ◽  
C. A. Rees ◽  
R. A. Kennis ◽  
D. L. Zoran ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Skin Surface ◽  
Hair Coat ◽  
Surface Lipids ◽  
Skin Surface Lipids ◽  
Total Fat ◽  
Fat Diets

A Novel Enzymatic Assay for the Quantification of Skin Surface Lipids

1996 ◽  
Vol 24 (1) ◽  
pp. 69-83 ◽  
Author(s):  
A Ito ◽  
K Kitamura ◽  
K Sato ◽  
H Akamatsu
Keyword(s):  
Fatty Acids ◽  
Menstrual Cycle ◽  
Free Fatty Acids ◽  
Skin Surface ◽  
Enzymatic Assay ◽  
Menstrual Phase ◽  
Accuracy Of Measurements ◽  
Wide Range ◽  
Surface Lipids ◽  
Skin Surface Lipids

The applicability of a novel enzymatic assay for quantifying skin surface lipids was investigated experimentally. The standard curves for the assays of glycerol esters, free fatty acids, and cholesterol and its esters were linear over a wide range of lipid concentrations, which ensures the accuracy of measurements. The assay system also showed good simultaneous reproducibility. There were significant positive correlations ( P < 0.001) between the quantities of glycerol esters, free fatty acids, and cholesterol and its esters sampled from the skin surface of women when measured by the enzymatic assay compared with the gas chromatographic method. The enzymatic assay was applied to studies of the relationships between age, acne and menstrual cycle, and skin surface lipids in women. The quantities of glycerol esters and free fatty acids reached peaks in females in their twenties and thirties. Increased quantities of glycerol esters, free fatty acids, and cholesterol and its esters were observed in women with acne compared with women without acne. Among the women with acne, those in the premenstrual phase of the menstrual cycle showed increased levels of glycerol esters, free fatty acids, and cholesterol and its esters compared with those in the menstrual phase. The results suggest that the enzymatic method is a satisfactory new technique for the quantification of skin surface lipids.

scholarly journals Lipidomics Profiling of Skin Surface Lipids in Senile Pruritus

2020 ◽  
Author(s):  
xiaolei Ma ◽  
Lulu Lu ◽  
Zheng Zhao ◽  
Mingru Cai ◽  
Na Gao ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Barrier Function ◽  
Skin Barrier ◽  
The Elderly ◽  
Skin Surface ◽  
Skin Barrier Function ◽  
Future Studies ◽  
Severity Scores ◽  
Surface Lipids ◽  
Skin Surface Lipids

Abstract Background: Senile pruritus is common, yet its etiology remains unknown. We examined the lipidomics profiles of skin surface lipids (SSL) in the elderly to better understand potential causes for senile pruritus. Methods:Transepidermal water loss (TEWL) was used to assess skin barrier function. Ameliorated Kawashima itch scale were used to measure the pruritus score. Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) and multivariate data analysis were used to investigate SSL alternations. Results:The results showed that the senile pruritus have higher TEWL values than controls (13.13±4.28 versus 6.71±2.45, p< 0.01). LC-MS/MS showed significant differences in lipidomics and identified 81 species of SSL that differ between two groups. Compared to controls, the levels of ceramides, diacylcerols, fatty acids, phosphatidylcholines, phosphatidylethar, phytosphingsines, sphingosines, diacylceryl-3-O-carboxyhydroxymethylcholine, diacylglyceryl trimethylhomoserine, unsaturated free fatty acids increased, whereas triacylglycerol decreased. CER-EOS, CER-NDS and CER-NS were positively correlated with TEWL values (p<0.05). Sphingomyelin, Cer-NP, Cer-AS, Cer-NDS, Cer-NS were positively correlated with pruritus severity scores, while Cer-BS, Cer-EODS, Cer-EOS, Cer-AP were negatively correlated. Conclusion:Our study indicated that the senile pruritus have impaired skin barrier function and altered SSL composition. Select SSL species identified in this study may be potential target for future studies on the pathogenesis of idiopathic senile pruritus

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