Studies on the effects of three antifeedants on the egg hatchability of Corcyra cephalonica Staint. (Galleriidae: Lepidoptera) and parasitism by Trichogramma australicum Gin. (Trichogrammatidae: Hymenoptera)

2009 ◽  
Vol 83 (1-4) ◽  
pp. 141-144 ◽  
Author(s):  
A. A. Kareem ◽  
S. Jayaraj ◽  
P. Thangavel ◽  
A. V. Navarajan Paul
Keyword(s):  
Corcyra Cephalonica ◽  
Egg Hatchability

scholarly journals Impact of combined action of Neem and Eucalyptus oil volatiles on different stages of Corcyra cephalonica (Lepidoptera: Pyralidae)

2011 ◽  
Vol 3 (2) ◽  
pp. 247-252 ◽  
Author(s):  
P. H. Pathak ◽  
Sangita Pandey
Keyword(s):  
Free Amino ◽  
Corcyra Cephalonica ◽  
Sharp Reduction ◽  
Eucalyptus Oil ◽  
Egg Hatchability ◽  
Rice Moth ◽  
Lepidoptera Pyralidae ◽  
Marked Decline ◽  
Combined Action ◽  
Limited Period

Combined action of neem (Azadiracta indica) and eucalyptus (Eucalyptus sp.) oil volatiles causes a sharp reduction in percent egg hatchability in rice moth, Corcyra cephalonica (Stainton) (Lepidoptera: pyralidae) when freshly laid eggs were exposed to these volatiles for 24 hours. A marked decline in egg output and egg hatchability in reproductive pairs, was observed whose larvae were allowed to develop in a programmed manner in an environment, laden with combined action of selected volumes of neem and eucalyptus oil or when both the sexes of these pyralids, were confined for the prescribed limited period, in such odorous environment. A significant reduction in glycogen, lipid and protein level and an increase in free amino acids was noticed in testes and ovaries of these pyralids, when breeding pairs were exposed to the selected volatiles for a period of six hours only.

scholarly journals Expression of Mst89B and CG31287 is Needed for Effective Sperm Storage and Egg Fertilization in Drosophila

Cells ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 289
Author(s):  
Gurman Grewal ◽  
Bahar Patlar ◽  
Alberto Civetta
Keyword(s):  
Germ Line ◽  
Sperm Storage ◽  
Mature Sperm ◽  
Sperm Production ◽  
Cytogenetic Map ◽  
Egg Hatchability ◽  
Storage Organs ◽  
Competitive Success ◽  
Paternity Success ◽  
And Storage

In Drosophila, male reproductive fitness can be affected by any number of processes, ranging from development of gametes, transfer to and storage of mature sperm within the female sperm storage organs, and utilization of sperm for fertilization. We have previously identified the 89B cytogenetic map position of D. melanogaster as a hub for genes that effect male paternity success when disturbed. Here, we used RNA interference to test 11 genes that are highly expressed in the testes and located within the 89B region for their role in sperm competition and male fecundity when their expression is perturbed. Testes-specific knockdown (KD) of bor and CSN5 resulted in complete sterility, whereas KD of CG31287, Manf and Mst89B, showed a breakdown in sperm competitive success when second to mate (P2 < 0.5) and reduced fecundity in single matings. The low fecundity of Manf KD is explained by a significant reduction in the amount of mature sperm produced. KD of Mst89B and CG31287 does not affect sperm production, sperm transfer into the female bursa or storage within 30 min after mating. Instead, a significant reduction of sperm in female storage is observed 24 h after mating. Egg hatchability 24 h after mating is also drastically reduced for females mated to Mst89B or CG31287 KD males, and this reduction parallels the decrease in fecundity. We show that normal germ-line expression of Mst89B and CG31287 is needed for effective sperm usage and egg fertilization.

A new feeding technique for experimental and routine culturing of the insect Rhodnius prolixus

1994 ◽  
Vol 72 (12) ◽  
pp. 2244-2247 ◽  
Author(s):  
Erwin Huebner ◽  
Rene Harrison ◽  
Karen Yeow
Keyword(s):  
Cost Effective ◽  
Rhodnius Prolixus ◽  
Wire Mesh ◽  
Artificial Membrane ◽  
Egg Hatchability ◽  
Rabbit Blood ◽  
Adult Females ◽  
Feeding Technique ◽  
Heat Sealing ◽  
Membrane Envelope

We report a method for artificially feeding the reduviid insect Rhodnius prolixus that utilizes heparinized rabbit blood enclosed in an artificial-membrane envelope. Envelopes of Parafilm M® or Nescofilm® are made by heat sealing two pieces, one of which is stretched first, on three sides. Blood is then put into the envelope, the air expelled, and the fourth side sealed with a bag sealer. The blood-filled envelopes are then placed on a standard slide warmer set at 37 °C and insects in jars with wire-mesh tops are allowed to feed. Normal gorging and subsequent molting of instars were attained. Adult females show normal fecundity (the E value was 0.19 for mated females) and egg hatchability was also normal. The method is simple, cost effective, and easy to implement. It is especially suited for experimental feedings.

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