Development of Creative Handicrafts Industrial Zones in Rajapolah District, Tasikmalaya Regency: Spatial Recommendation for Village Areas

The creative craft industry in Rajapolah District as the main driver of the regional economy in supporting facilities that still concentrated in one particular area. There have been limited studies concerned on spatial functions in creative spaces. This article addresses the criteria for functions and facilities for the development of creative industrial areas and their application in spatial recommendation for village areas. This research used a quantitative method using instruments on statistical data. Data analysis was divided into three stages namely identification, determining the criteria, and determining spatial functions. The study used the form of production data includes raw materials, labor, product size, product diversity, as well as the distribution of production houses, marketing facilities and education and training. The results showed that the development of creative industrial zones can be identified on criteria functions in the provision of raw materials, production, research and development, and marketing. Some villages have fulfilled the criteria for the function of creative spaces, which are relatively scattered in craft production houses close to the center of village activities. Besides that, it is also relatively far away, however supported by adequate road access. Creative promotion centers in the form of markets, outlets and shops are mostly concentrated on the main road network. Meanwhile, creative education centers in the form of multi-purpose building are relatively concentrated in some villages. Articles contribute to the field of creative industry science which is practically used as a consideration for the government in preparing detailed urban masterplans. Keywords: Creative industry; Handicrafts; Spatial; Development zone Copyright (c) 2021 Geosfera Indonesia and Department of Geography Education, University of Jember This work is licensed under a Creative Commons Attribution-Share A like 4.0 International License

PET PRODUCTS: RANGE AND CLASSIFICATION CRITERIA

Background. The market of goods for pets in Ukraine is promising and is characterized by rapid growth.The pet goods assortment is very wide and includes different subgroups. However, there is no works on the classification of the assortment of pet goods, except for food for them. The aim of the article is developing a classification of pet goods on base of the analysis of assortment. Materials and methods. General scientific methods of cognition: analysis, comparison and generalization were used during the research. Information base of the research: the range of products for animals presented in online stores. Results. According to the results of the analysis of the range of goods for animals in specialized and universal online stores, 9 types of goods for the transportation of pets have been identified.Goods for the transportation of pets can be classified according to the method of transportation and type of transport, type of goods, material from which it is made, size of the product, design features and other characteristics. The main classification features of goods for the arrangement of places of pet residence were determined: the type of goods, the type of animals for which it is intended, the material from which the product is made, the size. Additional classification features include design, equipment, the possibility of transformation, the presence of additional functions. The main classification features of goods for pet food were defined: purpose, type of animals, the material from which the product is made, type of product, size. Toilets fillers for pets can be classified by type of animals, type (material) of filler, functions (principle of action) of filler, size of fractions, the presence of flavor. The assortment of tools for dog and cat grooming includes different devices and acessories, which can be classified according to different characteristics depending on the type of product. The goods related to ammunition for dogs and cats can be classified by type of product, breed and/or size of the animal, the material from which the products are made, size, and other characteristics depending on the features of the type of product. Conclusion. The classification of goods for transportation, arrangement of accommodation and food, ammunition and toys for pets, which were developed in the work, indicates that their assortment is complex and can be classified on at least 5 signs.

Comparison of Biochemical Characteristics, Action Models, and Enzymatic Mechanisms of a Novel Exolytic and Two Endolytic Lyases with Mannuronate Preference

Recent explorations of tool-like alginate lyases have been focused on their oligosaccharide-yielding properties and corresponding mechanisms, whereas most were reported as endo-type with α-L-guluronate (G) preference. Less is known about the β-D-mannuronate (M) preference, whose commercial production and enzyme application is limited. In this study, we elucidated Aly6 of Flammeovirga sp. strain MY04 as a novel M-preferred exolytic bifunctional lyase and compared it with AlgLs of Pseudomonas aeruginosa (Pae-AlgL) and Azotobacter vinelandii (Avi-AlgL), two typical M-specific endolytic lyases. This study demonstrated that the AlgL and heparinase_II_III modules play indispensable roles in determining the characteristics of the recombinant exo-type enzyme rAly6, which is preferred to degrade M-enriched substrates by continuously cleaving various monosaccharide units from the nonreducing end, thus yielding various size-defined ΔG-terminated oligosaccharides as intermediate products. By contrast, the endolytic enzymes Pae-rAlgL and Avi-rAlgL varied their action modes specifically against M-enriched substrates and finally degraded associated substrate chains into various size-defined oligosaccharides with a succession rule, changing from ΔM to ΔG-terminus when the product size increased. Furthermore, site-directed mutations and further protein structure tests indicated that H195NHSTW is an active, half-conserved, and essential enzyme motif. This study provided new insights into M-preferring lyases for novel resource discoveries, oligosaccharide preparations, and sequence determinations.

Study association of bPRL (Bovine Prolactin) gene and milk production trait in Indonesian Holstein dairy cattle

This study was aimed to analyze the genetic variation of bPRL (Bovine Prolactin) gene and its association to milk production in Indonesian Friesian Holstein (IFH) dairy cattle. In total 49 DNA from whole blood were extracted and followed with PCR-RFLP to distinguish the genotype difference between individual samples. The fragment of bPRL gene was amplified using pair of primer forward 5’-GAGTCCTTATGAGCTTGATTCTT-3’ and reverse 5’-GCCTTCCAGAAGTCGTTTGTTTTC-3’, resulting in 156 bp PCR product size. DNA digestion then performed using RsaI restriction enzyme that recognizes 5’-GT↓AC-3’ sites with blunt-end termini. According to the study, two genotypes were discovered that are AA and AB, with respective frequencies of 0.84 and 0.16. Based on the genotype grouping of milk production data, AB genotype yielded 4016.63±1344 kg while AA genotype yielded 3314.32±1153 Kg of milk per lactation. However, there is no statistical (P>0,05) difference between both genotypes on the amount of milk yielded. It is concluded that bPRL gene was not associated with milk production traits in the studied Indonesian Dairy Cattle population.

Bacteriological and molecular characterization of Mycobacterium bovis isolates from tuberculous lesions collected among slaughtered cattle, Northwest Ethiopia

Background In Ethiopia, the distribution of bovine tuberculosis (BTB) has long been known and documented as a major problem of animal health. However, the burden of circulating M. bovis strains is poorly understood in the country. Therefore; this study aimed to identify and characterize the mycobacterial isolates responsible for BTB in Northwest Ethiopia. Methods A cross-sectional study was conducted on tuberculous lesions that had been collected from slaughtered cattle between September 2018 to June 2019. Collected lesions were cultured and tested for tuberculous bacilli. The MPT64 assay and Genotype line probe assay (LPA) were used for identification of mycobacterial isolates, and region of deletion 4 (RD4) typing and spoligotyping were used to characterize the M. bovis strains. Results Of the total 1458 examined slaughtered cattle, only 62 (4.3, 95%CI; 0.0328–0.0542) had tuberculous lesions. The highest number of gross tuberculous lesions were observed from the lymph nodes of the thoracic cavity; at the mediastinal (40.3%, 25/62) and bronchial (22.6%, 14/62) lymph nodes. Of the 62 collected tuberculous lesions; 18 (29.0%) were culture positive for mycobacterium isolates, and only five isolates were confirmed for M. tuberculosis complex (MTBc) by the MPT64 assay and LPA. All the five MTBc isolates were positive for RD4 typing of M. bovis with a PCR product size of 446 bp, and no isolate was noticed to have M. tuberculosis. The detected M. bovis strains displayed five spoligotypes; with the common SB1176 and SB0133 M. bovis strains, although the two spoligotypes had not been previously reported. Conclusion The present study shows that BTB in North Gondar, Ethiopia, is caused by M. bovis strains SB1176 and SB0033, with low frequency. Thus, the finding highlights the importance of continuous surveillance for mycobacterial strains in cattle populations.

Ocean Sound Analysis Software for Making Ambient Noise Trends Accessible (MANTA)

Making Ambient Noise Trends Accessible (MANTA) software is a tool for the community to enable comparisons between soundscapes and identification of ambient ocean sound trends required by ocean stakeholders. MANTA enhances the value of individual datasets by assisting users in creating thorough calibration metadata and internationally recommended products comparable over time and space to ultimately assess ocean sound at any desired scale up to a global level. The software package combines of two applications: MANTA Metadata App, which allows users to specify information about their recordings, and MANTA Data Mining App, which applies that information to acoustic recordings to produce consistently processed, calibrated time series products of sound pressure levels in hybrid millidecade bands. The main outputs of MANTA are daily.csv and NetCDF files containing 60-s spectral energy calculations in hybrid millidecade bands and daily statistics images. MANTA data product size and formats enable easy and compact transfer and archiving among researchers and programs, allowing data to be further averaged and explored to address user-specified questions.

Development of Novel Coconut SSR Markers Derived From Genome-Wide Bioinformatics Prediction

In the past, simple sequence repeat (SSR) marker development in coconut is achieved through microsatellite probing in bacterial artificial chromosome (BAC) clones or using previously developed SSR markers from closely related genomes. These coconut SSR markers are publicly available in published literatures and online databases; however, the number is quite limited. Here, we used a locally established, coconut genome-wide SSR prediction bioinformatics pipeline to generate a vast amount of coconut SSR markers. A total of 7,139 novel SSR markers were derived from the genome assembly of coconut ‘Catigan Green Dwarf’ (CATD). A subset of the markers, amounting to 131, were selected for synthesis based on motif filtering, contig distribution, product size exclusion, and success of in silico PCR in the CATD genome assembly. OligoAnalyzer-tool was also employed using the following desired parameters: %GC: 40–60%; minimum ΔG value for hairpin loop: -0.3 kcal/mol; minimum ΔG value for self-dimer: -0.9 kcal/mol; and minimum ΔG value for hetero-dimer: -0.9 kcal/mol. We have successfully synthesized, optimized, and amplified 131 novel SSR markers in coconut using ‘Catigan Green Dwarf’ (CATD), ‘Laguna Tall’ (LAGT), ‘West African Tall’ (WAT), and SYNVAR (LAGT x WAT) genotypes. Of the 131 SSR markers, 113 were polymorphic among the analyzed coconut genotypes. The development of novel SSR markers for coconut will serve as a valuable resource for mapping of quantitative trait loci (QTLs), assessment of genetic diversity and population structure, hybridity testing, and other marker-assisted plant breeding applications.

Development of Advanced Technologies for the Production of Innovative Materials with a Submicrocrystalline Structure by Methods of Severe Plastic Deformation

A promising direction for the development of steel and alloy processing processes is the intensification of plastic deformation by creating zones of localization of shear strains not only in the longitudinal but also in the transverse directions of the deformed metal flow. Intensification of alternating deformations along the entire cross-section and, especially, in the axial zone of the billet by creating new deformation schemes is an effective way to increase the physical, mechanical and functional properties of the metal with the maximum approximation of the finished product size to the original billet size. The paper shows that a promising idea is the development of new technological schemes that implement severe alternating deformation in existing metal forming processes. A continuous rolling method of wide strips is proposed, which provides severe alternating deformation with minor changes in the size of the billet. Based on this method, a scheme of continuous rolling of the strip with the intensification of plastic deformation of the metal is designed. The results of computer simulation showed that the new rolling method increases the strain uniformity in height and the value of the strain degree in the plane of symmetry of the billet.

Investigation of Lateral Confinement, Roller Aspect Ratio and Wear Condition on HPGR Performance Using DEM-MBD-PRM Simulations

It has been known that the performance of high-pressure grinding rolls (HPGR) varies as a function of the method used to laterally confine the rolls, their diameter/length (aspect) ratio as well as their condition, if new or worn. However, quantifying these effects through direct experimentation in machines with reasonably large dimensions is not straightforward, given the challenge, among others, of guaranteeing that the feed material remains unchanged. The present work couples the discrete element method (DEM) to multibody dynamics (MBD) and a novel particle replacement model (PRM) to simulate the performance of a pilot-scale HPGR grinding pellet feed. It shows that rotating side plates, in particular when fitted with studs, will result in more uniform forces along the bed, which also translates in a more constant product size along the rolls as well as higher throughput. It also shows that the edge effect is not affected by roll length, leading to substantially larger proportional edge regions for high-aspect ratio rolls. On the other hand, the product from the center region of such rolls was found to be finer when pressed at identical specific forces. Finally, rolls were found to have higher throughput, but generate a coarser product when worn following the commonly observed trapezoidal profile. The approach often used in industry to compensate for roller wear is to increase the specific force and roll speed. It has been demonstrated to be effective in maintaining product fineness and throughput, as long as the minimum safety gap is not reached.

Development of Novel Coconut Ssr Markers Derived From Genome-Wide Bioinformatics Prediction

In the past, simple sequence repeat (SSR) marker development in coconut is achieved through microsatellite probing in bacterial artificial chromosome (BAC) clones or using previously developed SSR markers from closely related genomes. These coconut SSR markers are publicly available in published literatures and online databases; however, the number is quite limited. Here, we used a locally established, coconut genome-wide SSR prediction bioinformatics pipeline to generate a vast amount of coconut SSR markers. A total of 7,139 novel SSR markers were derived from the genome assembly of coconut ‘Catigan Green Dwarf’ (CATD). A subset of the markers, amounting to 131, were selected for synthesis based on motif filtering, contig distribution, product size exclusion, and success of in silico PCR in the CATD genome assembly. OligoAnalyzer-tool was also employed using the following desired parameters: %GC: 40–60%; minimum ΔG value for hairpin loop: -0.3 kcal/mol; minimum ΔG value for self-dimer: -0.9 kcal/mol; and minimum ΔG value for hetero-dimer: -0.9 kcal/mol. We have successfully synthesized, optimized, and amplified 131 novel SSR markers in coconut using ‘Catigan Green Dwarf’ (CATD), ‘Laguna Tall’ (LAGT), ‘West African Tall’ (WAT), and SYNVAR (LAGT x WAT) genotypes. Of the 131 SSR markers, 113 were polymorphic among the analyzed coconut genotypes. The development of novel SSR markers for coconut will serve as a valuable resource for mapping of quantitative trait loci (QTLs), assessment of genetic diversity and population structure, hybridity testing, and other marker-assisted plant breeding applications.