Quantitative reverse transcription-PCR assay for the rapid detection of methicillin-resistantStaphylococcus aureus

2010 ◽  
Vol 108 (3) ◽  
pp. 779-788 ◽  
Author(s):  
M. Wada ◽  
E. Lkhagvadorj ◽  
L. Bian ◽  
C. Wang ◽  
Y. Chiba ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Rapid Detection ◽  
Pcr Assay ◽  
Quantitative Reverse Transcription Pcr ◽  
Reverse Transcription Pcr

Real-time quantitative reverse transcription-PCR assay for renal cell carcinoma-associated antigen G250

2002 ◽  
Vol 318 (1-2) ◽  
pp. 33-40 ◽  
Author(s):  
Ye Chuanzhong ◽  
Guan Ming ◽  
Zhang Fanglin ◽  
Chen Haijiao ◽  
Lin Zhen ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Real Time ◽  
Reverse Transcription ◽  
Renal Cell ◽  
Pcr Assay ◽  
Quantitative Reverse Transcription Pcr ◽  
Reverse Transcription Pcr

scholarly journals Implementation of an in-house real-time reverse transcription-PCR assay for the rapid detection of the SARS-CoV-2 Marseille-4 variant

2021 ◽  
pp. 104814
Author(s):  
Marielle Bedotto ◽  
Pierre-Edouard Fournier ◽  
Linda Houhamdi ◽  
Anthony Levasseur ◽  
Jeremy Delerce ◽  
...  
Keyword(s):  
Real Time ◽  
Reverse Transcription ◽  
Rapid Detection ◽  
Pcr Assay ◽  
Reverse Transcription Pcr

scholarly journals Quantitative Reverse Transcription-PCR Assay for Detection of mRNA Encoding Full-Length Human Tissue Kallikrein 7: Prognostic Relevance of KLK7 mRNA Expression in Breast Cancer 3

2006 ◽  
Vol 52 (6) ◽  
pp. 1070-1079 ◽  
Author(s):  
Leon Holzscheiter ◽  
Julia C Biermann ◽  
Matthias Kotzsch ◽  
Panagiotis Prezas ◽  
Juliane Farthmann ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Mrna Expression ◽  
Reverse Transcription ◽  
Human Tissue ◽  
Full Length ◽  
Tissue Kallikrein ◽  
Pcr Assay ◽  
Cancer Disease ◽  
Quantitative Reverse Transcription Pcr ◽  
Reverse Transcription Pcr

Abstract Background: The human tissue kallikrein gene family (KLK1 to KLK15) encodes a group of 15 serine proteases (hK1 to hK15), several of which have been implicated in cancer-related processes. Methods: We established a specific quantitative reverse transcription-PCR assay for full-length KLK7 mRNA that excluded amplification of the exon 2 deletion splice variant (the latter does not encode a functional protease), and evaluated full-length KLK7 mRNA expression [normalized to human glucose-6-phosphate dehydrogenase (h-G6PDH)] in tumor tissue specimens from 155 breast cancer patients. Results: High KLK7 mRNA expression (continuous) was significantly associated with a better patient outcome according to both univariate (P = 0.005) and multivariate (P = 0.046) Cox survival analysis. Separation of patients by optimized dichotomization revealed a significantly better prognosis for patients with high KLK7 mRNA status (n = 89) compared with patients with low KLK7 mRNA status (n = 66) [univariate hazard ratio (HR) = 0.45 (P = 0.001); multivariate HR = 0.50 (P = 0.005)]. In the subgroup of patients not receiving adjuvant treatment (n = 69), KLK7 mRNA status was a significant prognosticator [univariate HR = 0.29 (P = 0.002); multivariate HR = 0.40 (P = 0.034)]. This subgroup was least influenced by postoperative treatment and thus best showed the impact of KLK7 expression on the natural course of breast cancer disease. Conclusion: Expression of full-length KLK7 mRNA may represent a new prognostic marker in breast cancer disease.

scholarly journals Antifungal Resistance of Candida glabrata Vaginal Isolates and Development of a Quantitative Reverse Transcription-PCR-Based Azole Susceptibility Assay

2008 ◽  
Vol 52 (9) ◽  
pp. 3424-3426 ◽  
Author(s):  
Scott E. Gygax ◽  
John-Paul Vermitsky ◽  
Sean G. Chadwick ◽  
Matthew J. Self ◽  
Jessica A. Zimmerman ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Gold Standard ◽  
Candida Glabrata ◽  
Fold Increase ◽  
Opportunistic Pathogen ◽  
Azole Resistance ◽  
Pcr Assay ◽  
Quantitative Reverse Transcription Pcr ◽  
Reverse Transcription Pcr ◽  
Susceptibility Assay

ABSTRACT A multiplex quantitative reverse transcription-PCR assay was developed to detect azole resistance in Candida glabrata, an important opportunistic pathogen that develops resistance rapidly. Resistance was defined as a ≥3-fold increase in CDR1 expression by this assay, which proved to be 100% sensitive and 95% specific in comparison to the gold standard broth microdilution assay.

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