Minipreparation of total cellular DNA is useful as an alternative molecular marker of mitochondrial DNA for the identification ofTrichophyton mentagrophytesandT. rubrum

Mycoses ◽  
1996 ◽  
Vol 39 (1-2) ◽  
pp. 31-35 ◽  
Author(s):  
T. Mochizuki ◽  
M. Uehara ◽  
T. Menon ◽  
S. Ranganathan
Keyword(s):  
Mitochondrial Dna ◽  
Molecular Marker ◽  
Cellular Dna

scholarly journals Mitochondrial DNA damage: Molecular marker of vulnerable nigral neurons in Parkinson's disease

2014 ◽  
Vol 70 ◽  
pp. 214-223 ◽  
Author(s):  
Laurie H. Sanders ◽  
Jennifer McCoy ◽  
Xiaoping Hu ◽  
Pier G. Mastroberardino ◽  
Bryan C. Dickinson ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Dna Damage ◽  
Parkinson's Disease ◽  
Mitochondrial Dna ◽  
Molecular Marker ◽  
Mitochondrial Dna Damage

Mitochondrial DNA: The Alternative Molecular Marker for Cancer Detection and Treatment

2016 ◽  
Vol 2 (1) ◽  
pp. 01-04 ◽  
Author(s):  
Richa Sahota ◽  
Pratibha Ramani ◽  
Karthikeyan Ramalingam ◽  
Samir Anand ◽  
Ramandeep Singh Gambhir
Keyword(s):  
Mitochondrial Dna ◽  
Molecular Marker ◽  
Cancer Detection

scholarly journals Kinetic complexity of chloroplastal deoxyribonucleic acid and mitochondrial deoxyribonucleic acid from higher plants

1969 ◽  
Vol 112 (5) ◽  
pp. 777-786 ◽  
Author(s):  
Richard Wells ◽  
Max Birnstiel
Keyword(s):  
Molecular Weight ◽  
Mitochondrial Dna ◽  
Gaussian Distribution ◽  
Deoxyribonucleic Acid ◽  
Nuclear Dna ◽  
Higher Plants ◽  
Buoyant Density ◽  
Cell Fractionation ◽  
Caesium Chloride ◽  
Cellular Dna

1. Chloroplasts and mitochondria were isolated by aqueous and non-aqueous cell-fractionation techniques. In a variety of higher plants the mitochondrial DNA bands in a caesium chloride gradient at 1·706g.cm.−3, whereas chloroplastal DNA has a buoyant density of 1·697g.cm.−3. 2. In total cellular DNA of moderate molecular weight, the chloroplastal DNA is found within the Gaussian distribution of the nuclear DNA and is not resolved as a satellite. 3. Both chloroplastal DNA and mitochondrial DNA from lettuce renature rapidly. 4. The kinetic complexity of mitochondrial DNA is > 108 daltons. 5. Chloroplastal DNA is made up from fast and slow renaturing sequences with kinetic complexities of 3×106 and 1·2×108 daltons respectively. 6. From the discrepancy between analytical and kinetic complexity it is concluded that chloroplastal DNA is extensively reiterated.

Nondestructive sampling of mitochondrial DNA from voles (Microtus)

1987 ◽  
Vol 65 (1) ◽  
pp. 175-180 ◽  
Author(s):  
Yves Plante ◽  
Peter T. Boag ◽  
Bradley N. White
Keyword(s):  
Mitochondrial Dna ◽  
Population Structure ◽  
Social Interactions ◽  
Nuclear Dna ◽  
Microtus Pennsylvanicus ◽  
Dna Polymorphisms ◽  
Biochemical Taxonomy ◽  
Meadow Voles ◽  
Blood Samples ◽  
Cellular Dna

We present two techniques for sampling mitochondrial DNA (mtDNA) without killing individual voles. Total cellular DNA was extracted from small blood samples (100–250 μL) and tail segments (2 cm long) collected from meadow voles (Microtus pennsylvanicus). Restriction fragment patterns produced by the restriction endonucleases HindIII, BamH1, and EcoR1 after hybridization with a probe of nick-translated mtDNA compared well with standard mtDNA assays. Both techniques can be used in the field, and should prove useful in biochemical taxonomy as well as in investigations of population structure, dispersal, and social interactions on both micro- and macro-geographic scales. The Southern blots produced by these procedures have the added advantage of being reuseable, so that nuclear DNA polymorphisms can be examined with appropriate probes.

Genetic Diversity and Place in the General Phylogeographic Structure of Capercaillie, Tetrao Urogallus (Galliformes, Phasianidae), from Belarus

2019 ◽  
Vol 53 (5) ◽  
pp. 385-398
Author(s):  
K. V. Homel ◽  
T. E. Pavlushchick ◽  
M. E. Nikiforov ◽  
E. E. Kheidorova ◽  
M. G. Dmitrenok ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Mitochondrial Dna ◽  
Molecular Marker ◽  
Human Activities ◽  
Phylogeographic Structure ◽  
Tetrao Urogallus ◽  
Biological Resource ◽  
Effective Population ◽  
Mitochondrial Dna Control Region ◽  
Intraspecific Structure

Abstract We report on the assessment of the level of genetic diversity of the capercaillie in Belarus. This species of birds is a valuable biological resource, and also acts as a natural indicator for the state of large forests and the degree of their disturbance by human activities. Two subspecies of the capercaillie —Tetrao urogallus major (C. L. Brehm, 1831) and Tetrao urogallus pleskei (Stegmann, 1926) have been described for Belarus. The first domain of the mitochondrial DNA control region was used as the molecular marker for the study. An additional assessment of the level of genetic diversity of the capercaillie was carried out by calculating the effective size of its population (Ne) in Belarus. We found that the absence of subspecific structure is characteristic for all samples of the capercaillie from Belarus. The data on the intraspecific structure and genetic diversity of the capercaillie from Belarus allows us to considerits population in the central and eastern parts of the country as stable and sustainable. This can be explained by its inclusion in the general phylogeographic structure of birds of the boreal lineage. The low values of the effective population size for the partially isolated capercaillie population from the western part of the country indicate the need for increased attention and further monitoring of that population.

scholarly journals NUCLEAR GENE DOSAGE EFFECTS ON MITOCHONDRIAL MASS AND DNA

1974 ◽  
Vol 61 (3) ◽  
pp. 565-574 ◽  
Author(s):  
Gary W. Grimes ◽  
Henry R. Mahler ◽  
Philip S. Perlman
Keyword(s):  
Mitochondrial Dna ◽  
Gene Dosage ◽  
Nuclear Gene ◽  
Diploid Cell ◽  
Volume Distribution ◽  
Mitochondrial Mass ◽  
Gene Dosage Effects ◽  
Dosage Effects ◽  
Cellular Dna ◽  
Diploid Cells

In order to assess the effect of nuclear gene dosage on the regulation of mitochondria we have studied serial sections of a set of isogenic haploid and diploid cells of Saccharomyces cerevisiae, growing exponentially in the absence of catabolite repression, and determined the amount of mitochondrial DNA per cell. Mitochondria accounted for 14% of the cytoplasmic and 12% of the total cellular volume in all cells examined regardless of their ploidy or their apparent stage in the cell cycle. The mean number of mitochondria per cell was 22 in the diploid and 10 in the haploids. The volume distribution appeared unimodal and identical in haploids and diploids. The mitochondrial DNA accounted for 12.6 ± 1.2% and 13.5 ± 1.3% of the total cellular DNA in the diploid and haploid populations, respectively. These values correspond to 3.6 x 10-15 g, 2.2 x 109 daltons, or 44 genomes (50 x 106 daltons each) per haploid and twice that per diploid cell. On this basis, the average mitochondrion in these cells contains four mitochondrial genomes in both the haploid and the diploid.

Sign in / Sign up

Export Citation Format

Share Document