scholarly journals Serotonin Type 1D Receptors (5HT1DR) are Differentially Distributed in Nerve Fibres Innervating Craniofacial Tissues

Cephalalgia ◽  
2008 ◽  
Vol 28 (9) ◽  
pp. 933-944 ◽  
Author(s):  
AM Harriott ◽  
MS Gold
Keyword(s):  
Female Rats ◽  
Temporalis Muscle ◽  
Nerve Fibres ◽  
Sensory Afferents ◽  
Neuropeptide Release ◽  
Migraine Pain ◽  
Calcitonin Gene ◽  
Site Of Action ◽  
Sympathetic Efferents ◽  
Therapeutic Profile

We tested the hypothesis that the 5HT1DR, the primary antinociceptive target of triptans, is differentially distributed in tissues responsible for migraine pain. The density of 5HT1DR was quantified in tissues obtained from adult female rats with Western blot analysis. Receptor location was assessed with immunohistochemistry. The density of 5HT1DR was significantly greater in tissues known to produce migraine-like pain (i.e. circle of Willis and dura) than in structures in which triptans have no antinociceptive efficacy (i.e. temporalis muscle). 5HT1DR-like immunoreactivity was restricted to neuronal fibres, where it colocalized with calcitonin gene-related peptide and tyrosine hydroxylase immunoreactive fibres. These results are consistent with our hypothesis that the limited therapeutic profile of triptans could reflect its differential peripheral distribution and that the antinociceptive efficacy reflects inhibition of neuropeptide release from sensory afferents. An additional site of action at sympathetic efferents is also suggested.

scholarly journals Immunohistochemical characterisation of neurons in the mandibular ganglion and nerve fibres supplying the porcine mandibular gland

2017 ◽  
Vol 61 (No. 7) ◽  
pp. 361-373 ◽  
Author(s):  
M. Klimczuk ◽  
P. Podlasz ◽  
W. Sienkiewicz ◽  
A. Franke-Radowiecka ◽  
A. Dudek ◽  
...  
Keyword(s):  
Mandibular Gland ◽  
Double Labelling ◽  
Nerve Terminals ◽  
Rt Pcr ◽  
Nerve Fibres ◽  
Chemical Coding ◽  
Pcr Product ◽  
Calcitonin Gene ◽  
Oxide Synthase ◽  
Cryostat Sections

The present study was designed to investigate the chemical coding of neurons in the mandibular ganglion (MGn) and nerve fibres supplying the porcine mandibular gland (MGl) with the use of immunofluorescence and RT-PCR. The cryostat sections from MGn and MGl were processed for double-labelling immunohistochemistry using antisera against vesicular acetylcholine transporter (VAChT), choline acetyltransferase (ChAT), dopamine β-hydroxylase (DβH), neuronal nitric oxide synthase (nNOS), vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY), galanin (GAL), substance P (SP) and calcitonin gene-related peptide (CGRP). The MGl was found to be richly supplied by VAChT-positive nerve fibres that surrounded intra- and interlobular salivary ducts. A large number of VAChT-immunoreactive (VAChT-IR) nerve terminals were also observed around acini. Many periductal and periacinar nerve fibres stained positive for DβH. Immunoreactivity to GAL, NPY or VIP was observed in an intermediate number of nerve terminals which were associated with both salivary ducts and acini. Double-immunostaining revealed that in MGn nearly all neurons stained positive for VAChT/ChAT (98.45 ± 0.59%, mean ± SEM) and nNOS (99.71 ± 0.18%). An intermediate number of the nerve cell bodies displayed immunoreactivity to NPY or VIP (18.67 ± 0.52% and 8.11 ± 0.36%, respectively). Single GAL-IR and CGRP-positive neurons were also observed. RT-PCR revealed the presence of transcripts of ChAT, VAChT, nNOS, NPY, VIP and GAL. For SP and DβH very weak signals were observed. RT-PCR with primers targeting CGRP did not generate any PCR product.

Tachykininergic mediation of viscerosensitive responses to acute inflammation in rats: role of CGRP

1997 ◽  
Vol 272 (1) ◽  
pp. G141-G146 ◽  
Author(s):  
V. Julia ◽  
L. Bueno
Keyword(s):  
Acetic Acid ◽  
Gastric Emptying ◽  
Visceral Pain ◽  
Abdominal Muscle ◽  
Sodium Chromate ◽  
Sensory Afferents ◽  
Neurokinin Receptors ◽  
Calcitonin Gene ◽  
Reconstituted Milk

Tachykinins, colocalized with calcitonin gene-related peptides (CGRP) in sensory afferents, are involved in viscerosensitive responses. We investigated the role of tachykinins and CGRP in both nociceptive and visceromotor responses to inflammation. Visceral pain was assessed by abdominal muscle contractions. Gastric emptying was evaluated after gavage with reconstituted milk containing 51Cr-labeled sodium chromate. Acetic acid or 9% NaCl was injected intraperitoneally before the meal. RP-67580, SR-48968, human CGRP [hCGRP-(8-37)], or their vehicles were injected before acetic acid or saline. RP-67580, SR-48968, or their vehicles were injected before CGRP and the meal. GR-73632 or GR-76349 was injected before the meal. Acetic acids inhibited gastric emptying and increased the number of abdominal contractions. RP-67580 reduced the inhibition of gastric emptying without affecting the abdominal response. SR-48968 only reduced the acetic acid-induced increase of abdominal contractions. hCGRP-(8-37) reduced both responses induced by acetic acid. CGRP mimicked the effects of acetic acid. RP-67580 abolished CGRP-induced gastric emptying inhibition, whereas SR-48968 only diminished visceral pain. GR-73632 reduced gastric emptying, and GR-64349 increased abdominal response. In inflammation, neurokinin receptors (NK1 and NK2) mediate the gastric emptying inhibition and visceral pain, respectively. These responses involve a release of CGRP.

CELLULAR LOCALIZATION OF A PROLACTIN-LIKE ANTIGEN IN THE RAT BRAIN

1979 ◽  
Vol 83 (2) ◽  
pp. 261-NP ◽  
Author(s):  
G. TOUBEAU ◽  
J. DESCLIN ◽  
M. PARMENTIER ◽  
J. L. PASTEELS
Keyword(s):  
Rat Brain ◽  
Cellular Localization ◽  
Female Rats ◽  
Nerve Fibres ◽  
Male And Female ◽  
Immunoreactive Material ◽  
Paraventricular Nuclei ◽  
Male And Female Rats ◽  
The Brain

The distribution of immunoreactive neurones and fibres was studied in rat brain using an antiserum to rat prolactin. Neurones containing the immunoreactive material were localized in the arcuate, ventromedial, premamillary, supraoptic and paraventricular nuclei of the hypothalamus. Immunoreactive nerve fibres were widely distributed within the brain. No differences were observed in labelling between male and female rats, or as a consequence of hypophysectomy.

THE RESPONSE OF 5′-NUCLEOTIDASE IN THE BRAIN TO OESTROGEN ADMINISTRATION IN THE GROWING RAT

1955 ◽  
Vol 12 (4) ◽  
pp. 231-235 ◽  
Author(s):  
D. NAIDOO ◽  
H. REY
Keyword(s):  
Normal Saline ◽  
Female Rats ◽  
Nucleotidase Activity ◽  
Nerve Fibres ◽  
Experimental Animals ◽  
Minimum Dose ◽  
Growing Rat ◽  
Similar Quantity ◽  
The Brain

SUMMARY After preliminary experiments to determine the minimum dose and optimum age at which invariable oestrogen-induced nucleotidase activity could be induced, six female rats aged 5 days were injected subcutaneously with 0·25 mg oestradiol monobenzoate for 10 days. Six female litter-mates injected with a similar quantity of normal saline were used as controls. The brains of these animals were examined histologically for 5′-nucleotidase activity. It was found that the activity of 5′-nucleotidase was increased considerably, the nerve fibres in the brains of the experimental animals being in this respect indistinguishable from the nerve fibres of normal 30-day-old animals, while the control animals showed no change from the normal. Further, nuclei which show poor 5′-nucleotidase activity in the normal 30-day-old rat were strongly active in the brains of 15-day-old experimental animals. In this respect there was no difference between the oestrogen-treated rats and controls of the same age. The significance of the results is discussed.

Diverse interactions of calcitonin gene related peptide and nitric oxide in the gastric and cutaneous microcirculation

1995 ◽  
Vol 73 (7) ◽  
pp. 991-994 ◽  
Author(s):  
P. Holzer ◽  
Ch. Wachter ◽  
M. Jocič ◽  
I. Th. Lippe ◽  
A. Heinemann ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Gastric Mucosa ◽  
Calcitonin Gene Related Peptide ◽  
Afferent Nerve ◽  
Nerve Fibres ◽  
Related Peptide ◽  
Neurogenic Vasodilatation ◽  
Cutaneous Microcirculation ◽  
Cgrp Release ◽  
Calcitonin Gene

Calcitonin gene related peptide (CGRP) is the major mediator of afferent nerve mediated vasodilatation in the gastric mucosa and skin of the rat. Since receptors for CGRP occur on both the vascular endothelium and smooth muscle, it is conceivable that the vascular actions of CGRP involve multiple mechanisms. The vasodilator effect of rat CGRP-α in the rat gastric mucosa is indeed inhibited by blockade of nitric oxide (NO) synthesis, as is the gastric mucosal hyperemia in response to gastric acid challenge, which is mediated by CGRP release from afferent nerve fibres. In contrast, the vasodilator response to rat CGRP-α in the rat hind paw and the CGRP-mediated vasodilatation evoked by antidromic stimulation of afferent nerve fibres do not depend on the formation of NO. These data indicate that NO plays regionally different roles in the local vasodilator action of CGRP. NO is a secondary vasorelaxant messenger of CGRP in the gastric, but not in the cutaneous, microcirculation. However, this L-arginine-derived autacoid may have a role in the irritant-induced CGRP release from afferent vasodilator fibres in the skin.Key words: calcitonin gene related peptide, nitric oxide, microcirculation, gastric mucosa, skin, afferent nerve fibres, neurogenic vasodilatation.

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