CELLULAR LOCALIZATION OF A PROLACTIN-LIKE ANTIGEN IN THE RAT BRAIN

1979 ◽  
Vol 83 (2) ◽  
pp. 261-NP ◽  
Author(s):  
G. TOUBEAU ◽  
J. DESCLIN ◽  
M. PARMENTIER ◽  
J. L. PASTEELS
Keyword(s):  
Rat Brain ◽  
Cellular Localization ◽  
Female Rats ◽  
Nerve Fibres ◽  
Male And Female ◽  
Immunoreactive Material ◽  
Paraventricular Nuclei ◽  
Male And Female Rats ◽  
The Brain

The distribution of immunoreactive neurones and fibres was studied in rat brain using an antiserum to rat prolactin. Neurones containing the immunoreactive material were localized in the arcuate, ventromedial, premamillary, supraoptic and paraventricular nuclei of the hypothalamus. Immunoreactive nerve fibres were widely distributed within the brain. No differences were observed in labelling between male and female rats, or as a consequence of hypophysectomy.

scholarly journals Chronic Developmental Lead Exposure increases μ-Opiate Receptor Levels in the Adolescent Rat Brain

2020 ◽  
Author(s):  
Damaris Albores-Garcia ◽  
Jennifer L McGlothan ◽  
Zoran Bursac ◽  
Tomás R. Guilarte
Keyword(s):  
Rat Brain ◽  
Early Adolescence ◽  
Drugs Of Abuse ◽  
Specific Binding ◽  
Biological Effects ◽  
The United States ◽  
Female Rats ◽  
Opioid Use ◽  
Male And Female Rats ◽  
The Brain

AbstractOpioid use and abuse has reached epidemic proportion in the United States resulting in a significant numbers of deaths due to overdose. While environmental factors are implicated in opioid addiction, less is known about the role of exposure to environmental pollutants on the brain opioid system. Human and preclinical studies have suggested an association between childhood lead (Pb2+) intoxication and proclivity to substance abuse and delinquent behavior. Opioid receptors are involved in the biological effects of opioids and other drugs of abuse. In this study, we examine the effect of chronic developmental Pb2+ exposure on μ-opioid receptor (MOR) levels in the rat brain using [3H]-D-Ala2-MePhe4-Gly-ol5 enkephalin ([3H]-DAMGO) quantitative receptor autoradiography.Our results indicate that chronic developmental Pb2+ exposure increases the levels of [3H]-DAMGO specific binding to MOR in several limbic regions of the brain in male and female rats during the pre-adolescence (PN14) and early-adolescence (PN28) period. These changes were less pronounced in late-adolescence (PN50) and adult (PN120) animals. Our findings are important because the pre-adolescence and early adolescence period is a time in which there is higher engagement in reward and drug seeking behaviors in humans.In summary, we show that chronic exposure to Pb2+ an ubiquitous and well-known environmental contaminant and neurotoxicant, alters MOR levels in brain regions associated with addiction circuits in the adolescent period with important implications to opioid drug use and abuse.

A model for the study of sexual function in anesthetized male and female rats

1991 ◽  
Vol 261 (5) ◽  
pp. R1276-R1285 ◽  
Author(s):  
K. E. McKenna ◽  
S. K. Chung ◽  
K. T. McVary
Keyword(s):  
Sexual Function ◽  
Penile Erection ◽  
Neural Mechanisms ◽  
Female Rats ◽  
Descending Inhibition ◽  
Male And Female ◽  
Uterine Contractions ◽  
Male And Female Rats ◽  
Rhythmic Contractions ◽  
The Brain

A preparation for the study of sexual function in anesthetized spinal male and female rats is discussed. Urethral stimulation in males elicited penile erection, ejaculation, and rhythmic contractions of the striated perineal muscles. In females, vaginal and uterine contractions and rhythmic contractions of the perineal muscles were elicited. These responses show many similarities to responses seen during sexual climax in unanesthetized humans and animals. This response, which we refer to as the urethrogenital reflex, is unaffected in either sex by gonadectomy. We determined that the urethrogenital reflex is produced by a spinal pattern generator and is under tonic descending inhibition from the brain stem. We conclude that this preparation may be a valuable model for experimental study of the neural mechanisms of sexual function in both sexes.

scholarly journals Molecular cloning and expression of novel sulphotransferase-like cDNAs from human and rat brain

2000 ◽  
Vol 346 (3) ◽  
pp. 857-864 ◽  
Author(s):  
Charles N. FALANY ◽  
Xiaowei XIE ◽  
Jin WANG ◽  
Jorge FERRER ◽  
Josie L. FALANY
Keyword(s):  
Rat Brain ◽  
Polyclonal Antibodies ◽  
Brain Regions ◽  
Female Rats ◽  
Cloning And Expression ◽  
Female Rat ◽  
Affinity Tag ◽  
Calculated Molecular Mass ◽  
Male And Female ◽  
Male And Female Rats

The sulphotransferase (SULT) gene family is involved with the conjugation of many small drugs, xenobiotics and endogenous compounds. In this report, we describe the cloning and expression of novel cDNAs from human and rat brain, which are structurally related to the SULTs. These cDNAs have been termed ‘brain sulphotransferase-like’ (BR-STL), because of their similarity to the SULTs and their selective expression in brain tissue. The proteins encoded by the human and rat BR-STL cDNAs (hBR-STL-1 and rBR-STL cDNA respectively), denoted as hBR-STL and rBR-STL, are 98% identical and 99% similar in sequence. The hBR-STL-1 cDNA contains an 852-nt open reading frame encoding a 284-amino-acid protein with a calculated molecular mass of 33083 Da. Northern-blot analyses of RNA isolated from eight human tissues indicate that the hBR-STL message is selectively expressed in brain. Characterization of different brain regions showed that message levels were highest in cortical brain regions. rBR-STL message levels were relatively low in brains of 1-day-old male and female rats, but increased to adult levels in RNA from 7-day-old rats, and remained at that level in adult animals. The hBR-STL and rBR-STL cDNAs were expressed in both Escherichia coli and Sf9 insect cells in the presence or absence of an N-terminal histidine-affinity tag (His-tag). Polyclonal antibodies were raised in chickens against purified His-tagged hBR-STL, and were used to detect the presence of rBR-STL in adult male and female rat brain cytosol. The high degree of sequence conservation, and the selective localization of the BR-STL message in brain, suggest an important function in the central nervous system.

Regulation of Pro-Gonadotropin-Releasing Hormone Gene Expression by Sex Steroids in The Brain of Male and Female Rats

1989 ◽  
Vol 3 (11) ◽  
pp. 1748-1756 ◽  
Author(s):  
Daniel Toranzo ◽  
Eric Dupont ◽  
Jacques Simard ◽  
Claude Labrie ◽  
Jacques Couet ◽  
...  
Keyword(s):  
Gene Expression ◽  
Sex Steroids ◽  
Gonadotropin Releasing Hormone ◽  
Female Rats ◽  
Male And Female ◽  
Releasing Hormone ◽  
Male And Female Rats ◽  
The Brain
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