Prevalence rates and antifungal susceptibility profiles of the Candida parapsilosis species complex: results from a nationwide surveillance of candidaemia in Brazil

Objective: Candida parapsilosis species complex and Lodderomyces elongisporus may have differences in terms of their virulence, prevalence, and antifungal susceptibility profiles. These species are difficult to identify with biochemical methods. Therefore, there is a need for more efficient identification methods in terms of time, cost, and applicability. This study aims to evaluate the diagnostic performance of the MALDI-TOF MS method in discriminating between isolates belonging to the C. parapsilosis species complex and L. elongisporus. Method: In the current study, a total of 32 reference strains, including the C. parapsilosis (n=8), Candida orthopsilosis (n=7), Candida metapsilosis (n=6), and L. elongisporus (n=11) species were identified using the MALDI-TOF MS method. Results: The species names of 31 (93.7%) isolates belonging to the C. parapsilosis species complex and L.elongisporus were correctly identified. Twenty four isolates including eight (100%) C. parapsilosis, five (83%) C. metapsilosis, five (71%) C. orthopsilosis, and six (54%) L. elongisporus isolates were identified with score values ranging from 1.7 to 2.14. According to the secure identification reference score of ≥ 1.7, the sensitivity and specificity of the MALDI-TOF MS method were determined as 54.5–100% and 96.3–100%, respectively. Conclusion: Although the MALDI-TOF MS method has been shown to be effective in the rapid molecular phenotypic diagnosis of species that were difficult to discriminate using biochemical methods such as C. parapsilosis species complex and L. elongisporus, there is a clear need to optimize the method and develop a larger MS library for species-level identification within secure score ranges.

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Prevalence, distribution and antifungal susceptibility profiles of Candida parapsilosis, Candida orthopsilosis and Candida metapsilosis bloodstream isolates

Journal of Medical Microbiology ◽

10.1099/jmm.0.037812-0 ◽

2012 ◽

Vol 61 (7) ◽

pp. 1003-1008 ◽

Cited By ~ 29

Author(s):

Lucas Xavier Bonfietti ◽

Marilena dos Anjos Martins ◽

Maria Walderez Szeszs ◽

Sandra Brasil Stolf Pukiskas ◽

Sonia Ueda Purisco ◽

...

Keyword(s):

Candida Parapsilosis ◽

Antifungal Susceptibility ◽

Candida Metapsilosis ◽

Candida Orthopsilosis ◽

Susceptibility Profiles

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Antifungal susceptibility and virulence of Candida parapsilosis species complex: an overview of their pathogenic potential

Journal of Medical Microbiology ◽

10.1099/jmm.0.000756 ◽

2018 ◽

Vol 67 (7) ◽

pp. 903-914 ◽

Cited By ~ 8

Author(s):

Raimunda Sâmia Nogueira Brilhante ◽

Jamille Alencar Sales ◽

Maria Lucilene Queiroz da Silva ◽

Jonathas Sales de Oliveira ◽

Lucas de Alencar Pereira ◽

...

Keyword(s):

Species Complex ◽

Candida Parapsilosis ◽

Antifungal Susceptibility ◽

Pathogenic Potential

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Prevalence and antifungal susceptibility profiles of Candida glabrata, Candida parapsilosis and their close-related species in oral candidiasis

Archives of Oral Biology ◽

10.1016/j.archoralbio.2018.07.017 ◽

2018 ◽

Vol 95 ◽

pp. 100-107 ◽

Cited By ~ 9

Author(s):

Katherine Miranda-Cadena ◽

Cristina Marcos-Arias ◽

Estibaliz Mateo ◽

José Manuel Aguirre ◽

Guillermo Quindós ◽

...

Keyword(s):

Related Species ◽

Candida Glabrata ◽

Candida Parapsilosis ◽

Oral Candidiasis ◽

Antifungal Susceptibility ◽

Susceptibility Profiles

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Frequency, virulence factors and antifungal susceptibility of Candida parapsilosis species complex isolated from patients with candidemia in the central region of Argentina

Journal de Mycologie Médicale ◽

10.1016/j.mycmed.2019.100907 ◽

2019 ◽

Vol 29 (4) ◽

pp. 285-291

Author(s):

C. Vigezzi ◽

P.A. Icely ◽

C. Dudiuk ◽

E. Rodríguez ◽

M.S. Miró ◽

...

Keyword(s):

Virulence Factors ◽

Central Region ◽

Species Complex ◽

Candida Parapsilosis ◽

Antifungal Susceptibility

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Antifungal susceptibility profiles of rare ascomycetous yeasts

Journal of Antimicrobial Chemotherapy ◽

10.1093/jac/dkz231 ◽

2019 ◽

Vol 74 (9) ◽

pp. 2649-2656 ◽

Cited By ~ 6

Author(s):

Antonio Pérez-Hansen ◽

Cornelia Lass-Flörl ◽

Michaela Lackner ◽

M Aigner ◽

A Alastruey-Izquierdo ◽

...

Keyword(s):

Species Complex ◽

Yeast Species ◽

Antifungal Susceptibility ◽

Candida Rugosa ◽

Microdilution Method ◽

Broth Microdilution Method ◽

Rare Yeast Species ◽

Ascomycetous Yeasts ◽

Susceptibility Profiles ◽

Susceptibility Patterns

AbstractObjectivesTo generate antifungal susceptibility patterns for Trichomonascus ciferrii (Candida ciferrii), Candida inconspicua (Torulopsis inconspicua) and Diutina rugosa species complex (Candida rugosa species complex), and to provide key parameters such as MIC50, MIC90 and tentative epidemiological cut-off values (TECOFFs).MethodsOur strain set included isolates of clinical origin: C. inconspicua (n = 168), D. rugosa species complex (n = 90) [Candida pararugosa (n = 60), D. rugosa (n = 26) and Candida mesorugosa (n = 4)], Pichia norvegensis (Candida norvegensis) (n = 15) and T. ciferrii (n = 8). Identification was performed by MALDI-TOF MS or internal transcribed spacer sequencing. Antifungal susceptibility patterns were generated for azoles, echinocandins and amphotericin B using commercial Etest and the EUCAST broth microdilution method v7.3.1. Essential agreement (EA) was calculated for Etest and EUCAST.ResultsC. inconspicua, C. pararugosa and P. norvegensis showed elevated azole MICs (MIC50 ≥0.06 mg/L), and D. rugosa and C. pararugosa elevated echinocandin MICs (MIC50 ≥0.06 mg/L). EA between methods was generally low (<90%); EA averaged 77.45%. TECOFFs were suggested for C. inconspicua and D. rugosa species complex.ConclusionsRare yeast species tested shared high fluconazole MICs. D. rugosa species complex displayed high echinocandin MICs, while C. inconspicua and P. norvegensis were found to have high azole MICs. Overall, the agreement between EUCAST and Etest was poor and therefore MIC values generated with Etest cannot be directly compared with EUCAST results.

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Molecular epidemiology and antifungal susceptibility profiles of clinical Cryptococcus neoformans/Cryptococcus gattii species complex

Journal of Medical Microbiology ◽

10.1099/jmm.0.001101 ◽

2020 ◽

Vol 69 (1) ◽

pp. 72-81 ◽

Cited By ~ 5

Author(s):

Zainab Bandalizadeh ◽

Tahereh Shokohi ◽

Hamid Badali ◽

Mahdi Abastabar ◽

Farhang Babamahmoudi ◽

...

Keyword(s):

Molecular Epidemiology ◽

Cryptococcus Neoformans ◽

Species Complex ◽

Antifungal Susceptibility ◽

Cryptococcus Gattii ◽

Susceptibility Profiles

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Candidaemia due to Candida parapsilosis species complex at a hospital in Brazil: Clinical characteristics and antifungal susceptibility profile

Revista Iberoamericana de Micología ◽

10.1016/j.riam.2016.06.008 ◽

2017 ◽

Vol 34 (2) ◽

pp. 106-108 ◽

Cited By ~ 6

Author(s):

Débora de Souza Olartechea de Alencar ◽

Rosianne Assis de Sousa Tsujisaki ◽

Fernanda Luiza Espinosa Spositto ◽

Maína de Oliveira Nunes ◽

Adriana Araújo de Almeida ◽

...

Keyword(s):

Species Complex ◽

Clinical Characteristics ◽

Candida Parapsilosis ◽

Antifungal Susceptibility

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Antifungal Susceptibility Profiles of Bloodstream Yeast Isolates by Sensititre YeastOne over Nine Years at a Large Italian Teaching Hospital

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.00285-15 ◽

2015 ◽

Vol 59 (7) ◽

pp. 3944-3955 ◽

Cited By ~ 53

Author(s):

Brunella Posteraro ◽

Teresa Spanu ◽

Barbara Fiori ◽

Flavio De Maio ◽

Elena De Carolis ◽

...

Keyword(s):

Candida Parapsilosis ◽

Antifungal Susceptibility ◽

Reference Method ◽

Candida Guilliermondii ◽

Wild Type ◽

Content Type ◽

Laboratory Activity ◽

Clinical Breakpoints ◽

Fluconazole Resistant ◽

Susceptibility Profiles

ABSTRACTSensititre YeastOne (SYO) is an affordable alternative to the Clinical and Laboratory Standards Institute (CLSI) reference method for antifungal susceptibility testing. In this study, the MICs of yeast isolates from 1,214 bloodstream infection episodes, generated by SYO during hospital laboratory activity (January 2005 to December 2013), were reanalyzed using current CLSI clinical breakpoints/epidemiological cutoff values to assign susceptibility (or the wild-type [WT] phenotype) to systemic antifungal agents. ExcludingCandida albicans(57.4% of all isolates [n= 1,250]), the most predominant species wereCandida parapsilosiscomplex (20.9%),Candida tropicalis(8.2%),Candida glabrata(6.4%),Candida guilliermondii(1.6%), andCandida krusei(1.3%). Among the non-Candidaspecies (1.9%), 7 wereCryptococcus neoformansand 17 were other species, mainlyRhodotorulaspecies. Over 97% ofCandidaisolates were susceptible (WT phenotype) to amphotericin B and flucytosine. Rates of susceptibility (WT phenotype) to fluconazole, itraconazole, and voriconazole were 98.7% inC. albicans, 92.3% in theC. parapsilosiscomplex, 96.1% inC. tropicalis, 92.5% inC. glabrata, 100% inC. guilliermondii, and 100% (excluding fluconazole) inC. krusei. The fluconazole-resistant isolates consisted of 6C. parapsilosiscomplex isolates, 3C. glabrataisolates, 2C. albicansisolates, 2C. tropicalisisolates, and 1Candidalusitaniaeisolate. Of the non-Candidaisolates, 2C. neoformansisolates had the non-WT phenotype for susceptibility to fluconazole, whereasRhodotorulaisolates had elevated azole MICs. Overall, 99.7% to 99.8% ofCandidaisolates were susceptible (WT phenotype) to echinocandins, but 3 isolates were nonsusceptible (either intermediate or resistant) to caspofungin (C. albicans,C. guilliermondii, andC. krusei), anidulafungin (C. albicansandC. guilliermondii), and micafungin (C. albicans). However, when the intrinsically resistant non-Candidaisolates were included, the rate of echinocandin nonsusceptibility reached 1.8%. In summary, the SYO method proved to be able to detect yeast species showing antifungal resistance or reduced susceptibility.

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