Assignment of eight human genomic DNA sequences detecting high frequency restriction fragment length polymorphisms

1991 ◽  
Vol 55 (2) ◽  
pp. 141-150
Author(s):  
N. K. SPURR ◽  
D. KELSELL ◽  
L. ROOKE ◽  
L. L. CAVALLI-SFORZA ◽  
A. BOWCOCK ◽  
...  
Keyword(s):  
Restriction Fragment ◽  
Dna Sequences ◽  
Fragment Length ◽  
High Frequency ◽  
Genomic Dna ◽  
Restriction Fragment Length Polymorphisms ◽  
Length Polymorphisms ◽  
Human Genomic ◽  
Human Genomic Dna ◽  
Restriction Fragment Length

Restriction fragment length polymorphisms of Anisakinae larvae

1989 ◽  
Vol 63 (4) ◽  
pp. 269-274 ◽  
Author(s):  
Kazuo Sugane ◽  
Liu Qing ◽  
Tadashi Matsuura
Keyword(s):  
Restriction Fragment ◽  
Fragment Length ◽  
Genomic Dna ◽  
Paratenic Host ◽  
Restriction Fragment Length Polymorphisms ◽  
Type I ◽  
Type Ii ◽  
Length Polymorphisms ◽  
Paratenic Hosts ◽  
Restriction Fragment Length

ABSTRACTThe analysis of restriction fragment length polymorphisms (RFLPs) was applied to distinguish several kinds of Anisakinae larvae, Anisakis larvae (type I) collected from two different paratenic hosts, Anisakis larvae (type II) and Contracaecum larvae. The patterns of the two different paratenic host-derived DNA of Anisakis larva (I) were exactly the same in hybridized fragments generated by six endonucleases. The quite different patterns in RFLPs of genomic DNA were observed among the Anisakis larva (I), Anisakis larva (II) and Contracaecum larvae. The results suggest that the RFLPs analysis may be useful for distinguishing Anisakinae larvae and clarifying the relationships between Anisakis larvae and their adult worms.

scholarly journals IDENTIFICATION OF ROSE CULTIVARS BY RESTRICTION FRAGMENT LENGTH POLYMORPHISMS

HortScience ◽  
1991 ◽  
Vol 26 (5) ◽  
pp. 484b-484
Author(s):  
Sriyani Rajapakse ◽  
Mark Hubbard ◽  
Albert Abbott ◽  
Robert Ballard ◽  
John Kelly
Keyword(s):  
Restriction Fragment ◽  
Fragment Length ◽  
Genomic Dna ◽  
Cultivar Identification ◽  
Restriction Fragment Length Polymorphisms ◽  
Repeated Sequences ◽  
Dna Library ◽  
Dichotomous Key ◽  
Length Polymorphisms ◽  
Restriction Fragment Length

Restriction Fragment Length Polymorphisms (RFLPs) were investigated in rose cultivars as a means of reliable cultivar identification. A random genomic DNA library was generated by shotgun cloning HindIII digested fragments of DNA extracted from rose cultivar Confection into pUC8 plasmid of Escherichia coli strain JM 83. Compared to genomic clones carrying low or highly repeated sequences, clones with moderately repeated sequences were most effective in cultivar identification. These clones were identified by hybridizing rose DNA fragments from the library with genomic DNA from `Confection'. Clones with moderately repeated copy sequences were used as probes to detect the presence of RFLPs by Southern hybridization of EcoRI digested genomic DNA of various rose cultivars. Several of these probes have revealed RFLPs useful in cultivar identification. By using a combination of two or more of these probes most of the rose cultivars compared at this time can be identified. A dichotomous key useful in identification of rose cultivars was prepared from RFLPs displayed by 3A9 probe.

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