REGULATION OF SEED-GERMINATION TIMING BY MOIST CHILLING IN WESTERN HEMLOCK

Various environmental factors were tested under laboratory conditions to determine their effects on germination of seeds of prickly sida (Sida spinosaL. ♯3SIDSP). Neither freezing and thawing nor moist chilling at 5 C promoted seed germination. However, increasing the incubation temperature and subjecting seeds to wet-dry cycles enhanced germination; high temperatures were more effective than alternate wetting and drying. Shifting seeds from a lower to a higher temperature regime increased germination. Seeds shifted from 15/6, 20/10, 25/15, or 30/15 C to higher regimes of 20/10, 25/15, 30/15, 35/20, or 40/25 C germinated to greater percentages than did seeds kept continuously at the lower thermoperiods. With an increase in length of time seeds were at a lower temperature, there was an increase in the percentage that germinated after they were moved to a higher regime.

Download Full-text

Morphological and germination response variability in seeds of wild yellow gentian (Gentiana lutea L.) accessions from northwest Spain

Botany ◽

10.1139/b2012-028 ◽

2012 ◽

Vol 90 (8) ◽

pp. 731-742 ◽

Cited By ~ 8

Author(s):

Félix Pérez-García ◽

Federico Varela ◽

M. Elena González-Benito

Keyword(s):

Seed Germination ◽

Water Content ◽

Germination Rate ◽

Seed Mass ◽

Germination Percentage ◽

Gentiana Lutea ◽

Moist Chilling ◽

Mean Germination Time ◽

Wild Accessions ◽

Seed Water Content

Gentiana lutea L. (yellow gentian, Gentianaceae) is an important medicinal plant under protection as endangered species in most European countries. The aim of this work was to evaluate variation in seed mass, seed water content, and seed germination among 56 wild accessions of G. lutea. The effect of gibberellic acid (GA3), putrescine, moist chilling, and level of ripeness of seeds on subsequent germination was also investigated. Seeds of G. lutea showed physiological dormancy (final germination percentages ranged from 0% to 11%, depending on the accession) and GA3 enhanced seed germination drastically in all the accessions. The highest germination (99%) of GA3-treated seeds was reached at 15 °C. Final germination percentage and germination rate (as expressed by mean germination time), as well as seed mass and seed water content, varied significantly among accessions. In general, 1 year moist chilling did not significantly enhance G. lutea seed germination. For most accessions, no significant differences were found between fully ripe seeds and less ripe seeds for seed water content, seed mass, and seed germination. Applications of GA3 were always most effective than those of putrescine for increasing seed germination.

Download Full-text

The Effect Mechanism of Moist-Chilling and GA3 on Seed Germination and Subsequent Seedling Growth of Ferula ovina Boiss

The Open Plant Science Journal ◽

10.2174/1874294700903010022 ◽

2009 ◽

Vol 3 (1) ◽

pp. 22-28 ◽

Cited By ~ 7

Author(s):

R. Amooaghaie

Keyword(s):

Seed Germination ◽

Seedling Growth ◽

Effect Mechanism ◽

Moist Chilling

Download Full-text

Seed Germination Traits of Loquat (Eriobotrya japonica Lindl.) As Affected by Various Pre-Sowing Treatments (Cutting of Cotyledons, Removal of Perisperm, Moist Chilling and/or Exogenous Application of Gibberellin)

Phyton ◽

10.32604/phyton.2020.010532 ◽

2020 ◽

Vol 89 (3) ◽

pp. 645-656

Author(s):

Thiresia-Teresa Tzatzani ◽

Evangelia Basdeki ◽

Evangelia-Vasiliki Ladikou ◽

Marios-Ioannis N. Sotiras ◽

Georgios Panagiotakis ◽

...

Keyword(s):

Seed Germination ◽

Eriobotrya Japonica ◽

Exogenous Application ◽

Moist Chilling ◽

Germination Traits

Download Full-text

The canonical RdDM pathway mediates the control of seed germination timing under salinity

The Plant Journal ◽

10.1111/tpj.15064 ◽

2020 ◽

Author(s):

Víctor Miguel Palomar ◽

Alejandro Garciarrubio ◽

Adriana Garay‐Arroyo ◽

Coral Martínez‐Martínez ◽

Omar Rosas‐Bringas ◽

...

Keyword(s):

Seed Germination ◽

Germination Timing

Download Full-text

Climate Warming Facilitates Seed Germination in Native but Not Invasive Solidago canadensis Populations

Frontiers in Ecology and Evolution ◽

10.3389/fevo.2020.595214 ◽

2020 ◽

Vol 8 ◽

Author(s):

Xiao-Hui Zhou ◽

Wei-Ming He

Keyword(s):

Seed Germination ◽

Climate Warming ◽

Seed Quality ◽

Germination Rate ◽

Plant Performance ◽

Solidago Canadensis ◽

Seed Traits ◽

Temperature Regimes ◽

Native Populations ◽

Germination Timing

Plant performance is commonly temperature-dependent so that this performance could vary with climate warming. Seeds are among the most important propagules of plants, and seed traits strongly influence plant invasion success. Therefore, understanding seed traits under climate warming is useful for predicting invasion risks. To this end, we conducted a warming experiment with an infrared radiator and examined the effects of 5 years warming (approximately 2°C above ambient) on the seed quality and subsequent germination of Solidago canadensis from North America, where it is native (24 native populations), and from China, where it is invasive (29 invasive populations). Temperature regimes (i.e., ambient vs. warming) interacted with population sources (i.e., native vs. invasive) to significantly influence seed germination, but not thousand-seed mass. Warming significantly advanced the seed germination timing of native S. canadensis populations and increased their seed germination rate; warming did not influence the germination timing but decreased the germination rate of invasive S. canadensis populations. Across two temperature regimes combined, 24 native S. canadensis populations had smaller seeds, later germination timing, and lower germination rate than 29 invasive S. canadensis populations. These findings suggest that climate warming could facilitate the seed germination of native but not invasive populations. Our data also highlight that invasive populations might be more successful than native populations due to better seed quality and faster and higher seed germination.

Download Full-text

Patterns of amplified restriction fragment polymorphism in the germination of Festuca hallii seeds

Seed Science Research ◽

10.1017/s0960258510000188 ◽

2010 ◽

Vol 20 (3) ◽

pp. 153-161

Author(s):

Jie Qiu ◽

Yuguang Bai ◽

Yong-Bi Fu ◽

John F. Wilmshurst

Keyword(s):

Genetic Variation ◽

Seed Germination ◽

Genetic Differentiation ◽

Restriction Fragment ◽

Population Based ◽

Canadian Prairie ◽

Band Frequency ◽

Restriction Fragment Polymorphism ◽

Festuca Hallii ◽

Germination Timing

AbstractTiming of seed germination influences plant lifetime fitness and can affect the ability of plant populations to colonize and persist in changing environments. However, the genetic variation of the seed germination response remains poorly understood. The amplified restriction fragment polymorphism (AFLP) technique was applied to characterize the genetic variation of germinated seeds collected from three Festuca hallii populations in the Canadian prairie. Three subpopulations with early, intermediate and late germination were identified from each population, based on germination tests at 10, 15 and 20°C in controlled growth chambers. Three AFLP primer pairs were employed to screen a total of 540 assayed seedling samples and 188 polymorphic AFLP bands were scored for each sample. None of the assayed AFLP bands were significantly associated with seed germination, but marked differences in estimates of mean band frequency were observed for various groups of germinating seeds under different test temperatures. Partitioning of the total AFLP variation showed that 5.9% AFLP variation was present among seeds of the three populations, 0.3% among seeds of three germination subpopulations, and 0.5% among seeds grouped for germination temperature. Genetic differentiation was significant among 27 groups of seeds representing population, germination timing and test temperature. Subpopulations with early and intermediate germination shared similar genetic backgrounds and were genetically differentiated from the late germination subpopulation. These results indicate that seed genotypes respond slightly differently to environmental variation, resulting in significant but weak genetic differentiation in the germination of F. hallii seeds. Implications for plant establishment and fescue restoration are discussed.

Download Full-text

Modelling seed germination response to temperature inEucalyptusL'Her. (Myrtaceae) species in the context of global warming

Seed Science Research ◽

10.1017/s0960258517000010 ◽

2017 ◽

Vol 27 (2) ◽

pp. 99-109 ◽

Cited By ~ 7

Author(s):

Anne Cochrane

Keyword(s):

Life History ◽

Seed Germination ◽

Western Australia ◽

Thermal Tolerance ◽

Greenhouse Gas Emission ◽

Climate Scenario ◽

Wet Season ◽

Physiological Tolerance ◽

Species Vulnerability ◽

Germination Timing

AbstractSeed germination is vital for persistence of many plant species, and is linked to local environmental conditions. Small increases in temperature during this critical life history transition may threaten species by altering germination timing and success. Such changes in turn may influence population dynamics, community composition and the geographic distributions of species. In this investigation, a bi-directional temperature gradient plate was used to profile thermal constraints for germination in 26 common, threatened and geographically restrictedEucalyptusspecies (Myrtaceae) from southern Western Australia. These observed data were used to populate models to predict optimum germination responses (mean time to germination, germination timing and success) under current (1950–2000 averages) and future (2070 high greenhouse gas emission climate scenario) mean monthly minimum and maximum temperatures. Many species demonstrated wide physiological tolerance for high germination temperatures and an ability to germinate outside current and forecast future autumn–winter wet season temperatures, suggesting that climatic distribution is a poor proxy for thermal tolerance forEucalyptusseed germination. Germination for some species is predicted to decline under forecast conditions, but the majority will maintain or improve germination particularly during the cooler winter months of the year. Although thermal tolerance may benefit persistence of manyEucalyptusspecies in southern Western Australia as warming becomes more severe, large rainfall declines are also forecast which may prove more detrimental to plant survival. Nonetheless, this framework has the potential to identify seed resilience to heat stress in an early life history phase and hence species vulnerability to one characteristic of forecast environmental change.

Download Full-text

Seed germination, seedling emergence, and seed bank ecology of sweet fern (Comptonia peregrina(L.) Coult.)

Canadian Journal of Botany ◽

10.1139/b99-081 ◽

1999 ◽

Vol 77 (9) ◽

pp. 1378-1386

Author(s):

Melissa A Dow ◽

Christa R Schwintzer

Keyword(s):

Seed Germination ◽

Seed Bank ◽

Temperature Fluctuation ◽

Seedling Emergence ◽

Chilling Temperature ◽

Secondary Dormancy ◽

Forest Sites ◽

Moist Chilling ◽

Adjacent Field ◽

Dormancy Cycles

We examined seed-bank seeds of sweet fern (Comptonia peregrina (L.) Coult.), an actinorhizal nitrogen-fixing shrub, to determine their distribution in the soil and to identify the factors that stimulate them to germinate following removal of the vegetation. Seeds were extracted from the soil of adjacent field and forest sites currently lacking sweet fern in Orono, Maine. Both sites contained approximately 2000 seeds·m-2with the greatest concentration at a depth of 60-80 mm. The seeds were 4.0-5.5 mm long, enclosed by a pitted, woody pericarp, and 8% contained embryos. Many seedlings emerged in disturbed plots (vegetation removed and upper soil mixed) in May and June 1997, but none appeared after 24 July. Seeds collected in May and June germinated readily in a growth chamber (30-45% germination) whereas only 2-5% of July- and August-collected seeds germinated indicating induction of secondary dormancy. August-collected seeds showed strong germination after >=15 days of moist chilling at 4°C indicating relief of secondary dormancy by chilling. Temperature fluctuation with an amplitude of 10°C strongly stimulated germination. Presence of annual secondary dormancy cycles and stimulation by strong temperature fluctuation assures that seed-bank seeds germinate under conditions that allow the seedlings to become established.Key words: actinorhizal plants, Comptonia peregrina, germination ecophysiology, secondary dormancy, seed bank, seedling emergence.

Download Full-text