ATPase activity, sodium and potassium content in guinea pig cortex after ouabain treatment in vivo

1973 ◽  
Vol 20 (1) ◽  
pp. 237-239 ◽  
Author(s):  
G. Venturini ◽  
G. Palladini
Keyword(s):  
Guinea Pig ◽  
Atpase Activity ◽  
Potassium Content ◽  
Sodium And Potassium

Water, Sodium, and Potassium Content of Human, Guinea Pig, and Rabbit Lung.

1953 ◽  
Vol 82 (2) ◽  
pp. 318-322 ◽  
Author(s):  
R. M. Mendenhall ◽  
P. M. Ramorino ◽  
B. Gerstl
Keyword(s):  
Guinea Pig ◽  
Potassium Content ◽  
Rabbit Lung ◽  
Sodium And Potassium

Effect of locally applied drugs on the pH of luminal fluid in the endolymphatic sac of guinea pig

2000 ◽  
Vol 279 (5) ◽  
pp. R1695-R1700 ◽  
Author(s):  
Vincent Couloigner ◽  
Marie Teixeira ◽  
Philippe Hulin ◽  
Olivier Sterkers ◽  
Maurice Bichara ◽  
...  
Keyword(s):  
Carbonic Anhydrase ◽  
Guinea Pig ◽  
Atpase Activity ◽  
Acid Secretion ◽  
Ph Sensitive ◽  
Endolymphatic Sac ◽  
Transport Systems ◽  
Membrane Bound ◽  
The Relationship

The aim of the present work was to assess the effect of various drugs applied locally on the pH of the luminal fluid (pHlum) in guinea pig endolymphatic sac. pHlum and transepithelial potential, when measured in vivo by means of double-barrelled pH-sensitive microelectrodes, were 7.06 ± 0.08 and +6.1 ± 0.34 mV (mean ± SE; n = 84), respectively, which is consistent with a net acid secretion in the luminal fluid of the endolymphatic sac. Bafilomycin and acetazolamide increased and decreased, respectively, pHlum. Amiloride, ethylisopropylamiloride, ouabain, and Schering 28080 had no effect on pHlum. Results obtained with inhibitors of anionic transport systems were inconclusive; e.g., DIDS reduced pHlum, whereas neither SITS nor triflocin had any effect. We conclude that bafilomycin-sensitive H+-ATPase activity accounts for the transepithelial acid gradient measured in the endolymphatic sac and that intracellular and membrane-bound carbonic anhydrase probably participates in regulating endolymphatic sac pHlum. The relationship between acid pH, endolymph volume, and Ménière's disease remains to be further investigated.

Inhibition of Human and Animal Platelet Adhesiveness to Glass Bead Columns by Adenosine, Dipyridamole, Chlorpromazine and Acetylsalicylic Acid

1976 ◽  
Vol 36 (02) ◽  
pp. 401-410 ◽  
Author(s):  
Buichi Fujttani ◽  
Toshimichi Tsuboi ◽  
Kazuko Takeno ◽  
Kouichi Yoshida ◽  
Masanao Shimizu
Keyword(s):  
Guinea Pig ◽  
Acetylsalicylic Acid ◽  
Guinea Pigs ◽  
Glass Bead ◽  
Animal Species ◽  
Inhibitory Effect ◽  
Rabbit Platelet ◽  
Platelet Adhesiveness

SummaryThe differences among human, rabbit and guinea-pig platelet adhesiveness as for inhibitions by adenosine, dipyridamole, chlorpromazine and acetylsalicylic acid are described, and the influence of measurement conditions on platelet adhesiveness is also reported. Platelet adhesiveness of human and animal species decreased with an increase of heparin concentrations and an increase of flow rate of blood passing through a glass bead column. Human and rabbit platelet adhesiveness was inhibited in vitro by adenosine, dipyridamole and chlorpromazine, but not by acetylsalicylic acid. On the other hand, guinea-pig platelet adhesiveness was inhibited by the four drugs including acetylsalicylic acid. In in vivo study, adenosine, dipyridamole and chlorpromazine inhibited platelet adhesiveness in rabbits and guinea-pigs. Acetylsalicylic acid showed the inhibitory effect in guinea-pigs, but not in rabbits.

In vivo activity of pyrimidine-dispirotripiperaziniumin in the male guinea pig model of genital herpes

2020 ◽  
Vol 09 (01) ◽  
Author(s):  
Novoselova EA ◽  
Alimbarova LM ◽  
Monakhova NS ◽  
Lepioshkin AY ◽  
Ekins S ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Genital Herpes ◽  
Pig Model ◽  
Guinea Pig Model

scholarly journals Optimization of spectral-domain optical coherence tomography with a supercontinuum source for in vivo motion detection of low reflective outer hair cells in guinea pig cochleae

2021 ◽  
Author(s):  
Fumiaki Nin ◽  
Samuel Choi ◽  
Takeru Ota ◽  
Zhang Qi ◽  
Hiroshi Hibino
Keyword(s):  
Optical Coherence Tomography ◽  
High Resolution ◽  
Guinea Pig ◽  
Hair Cells ◽  
Sensory Epithelium ◽  
Outer Hair Cells ◽  
Acquisition Time ◽  
Total Acquisition Time ◽  
Sd Oct

AbstractSound evokes sub-nanoscale vibration within the sensory epithelium. The epithelium contains not only immotile cells but also contractile outer hair cells (OHCs) that actively shrink and elongate synchronously with the sound. However, the in vivo motion of OHCs has remained undetermined. The aim of this work is to perform high-resolution and -accuracy vibrometry in live guinea pigs with an SC-introduced spectral-domain optical coherence tomography system (SD-OCT). In this study, to reveal the effective contribution of SC source in the recording of the low reflective materials with the short total acquisition time, we compare the performances of the SC-introduced SD-OCT (SCSD-OCT) to that of the conventional SD-OCT. As inanimate comparison objects, we record a mirror, a piezo actuator, and glass windows. For the measurements in biological materials, we use in/ex vivo guinea pig cochleae. Our study achieved the optimization of a SD-OCT system for high-resolution in vivo vibrometry in the cochlear sensory epithelium, termed the organ of Corti, in mammalian cochlea. By introducing a supercontinuum (SC) light source and reducing the total acquisition time, we improve the axial resolution and overcome the difficulty in recording the low reflective material in the presence of biological noise. The high power of the SC source enables the system to achieve a spatial resolution of 1.72 ± 0.00 μm on a mirror and reducing the total acquisition time contributes to the high spatial accuracy of sub-nanoscale vibrometry. Our findings reveal the vibrations at the apical/basal region of OHCs and the extracellular matrix, basilar membrane.

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