Superoxide Radical-Mediated Alteration of Synaptosome Membrane Structure and High-Affinity ?-[14C]Aminobutyric Acid Uptake

1986 ◽  
Vol 47 (6) ◽  
pp. 1804-1813 ◽  
Author(s):  
Edmund A. Debler ◽  
Henry Sershen ◽  
Abel Lajtha ◽  
Joseph F. Gennaro
Keyword(s):  
Membrane Structure ◽  
Superoxide Radical ◽  
Aminobutyric Acid ◽  
Acid Uptake ◽  
High Affinity ◽  
Synaptosome Membrane

scholarly journals Inhibition of high-affinity gamma-aminobutyric acid uptake in primary astrocyte cultures by phorbol esters and phospholipase C

1991 ◽  
Vol 275 (2) ◽  
pp. 435-439 ◽  
Author(s):  
J Gomeza ◽  
M Casado ◽  
C Gimenez ◽  
C Aragon
Keyword(s):  
Phospholipase C ◽  
Glial Cells ◽  
Kinase Inhibitor ◽  
Primary Cultures ◽  
Aminobutyric Acid ◽  
Gaba Uptake ◽  
Acid Uptake ◽  
Gamma Aminobutyric Acid ◽  
Gaba Transport ◽  
High Affinity

The effects of phorbol 12-myristate 13-acetate (PMA), a potent activator of protein kinase C (PKC), on high-affinity Na(+)-dependent gamma-aminobutyric acid (GABA) uptake were investigated in primary cultures of neurons and glial cells from rat brain cortex. Incubation of glial cells with PMA led to concentration- and time-dependent decreases in the GABA transport in glial cells. This effect could be completely suppressed by addition of the PKC inhibitor H7. The PMA effects could be mimicked by oleoylacetylglycerol, the diacylglycerol kinase inhibitor R59022 and exogenous phospholipase C. Treatment with PMA did not affect GABA transport in neuronal cells.

Association of [3H]nipecotic acid binding with the high-affinity γ-aminobutyric acid uptake system in human brain

1986 ◽  
Vol 14 (6) ◽  
pp. 1247-1248
Author(s):  
MICHAEL D. C. SIMPSON ◽  
ALAN J. CROSS ◽  
J. F. WILLIAM DEAKIN ◽  
PAUL SLATER
Keyword(s):  
Human Brain ◽  
Aminobutyric Acid ◽  
Acid Uptake ◽  
Uptake System ◽  
Nipecotic Acid ◽  
High Affinity

(R)-N-[4,4-Bis(3-Methyl-2-Thienyl)but-3-en-1-yl]Nipecotic Acid Binds with High Affinity to the Brain ?-Aminobutyric Acid Uptake Carrier

1990 ◽  
Vol 54 (2) ◽  
pp. 639-647 ◽  
Author(s):  
Claus Braestrup ◽  
Erik B. Nielsen ◽  
Ursula Sonnewald ◽  
Lars J. S. Knutsen ◽  
Knud Erik Andersen ◽  
...  
Keyword(s):  
Aminobutyric Acid ◽  
Acid Uptake ◽  
Nipecotic Acid ◽  
High Affinity ◽  
The Brain

Systemic CI-966, a new γ-aminobutyric acid uptake blocker, enhances γ-aminobutyric acid action in CA1 pyramidal layer in situ

1990 ◽  
Vol 68 (9) ◽  
pp. 1194-1199 ◽  
Author(s):  
U. Ebert ◽  
K. Krnjević
Keyword(s):  
Aminobutyric Acid ◽  
Gaba Uptake ◽  
Acid Uptake ◽  
Sprague Dawley ◽  
Nipecotic Acid ◽  
Ca1 Region ◽  
Stratum Pyramidale ◽  
Sprague Dawley Rats ◽  
Pyramidal Layer ◽  
Urethane Anaesthesia

A new potent, blood–brain barrier permeable γ-aminobutyric acid (GABA) uptake blocker, 1-[2-[bis[4-(trifluoromethyl)-phenyl]methoxy]ethyl]-1,2,5,6-tetrahydro-3-pyridinecarboxylic acid (CI-966) was administered systemically by i.p. injection (5 mg/kg) in Sprague–Dawley rats under urethane anaesthesia. Twenty to thirty minutes after injection there was a highly variable, but overall significant, enhancement of the inhibition of hippocampal population spikes by GABA applied by microiontophoresis in the CA1 region. Like the effect of nipecotic acid (applied locally by iontophoresis), the potentiation by CI-966 was clearest when GABA was applied in or near the stratum pyramidale where its action normally is weakest and shows the most pronounced fading. This change in GABA potency is most simply explained by a reduction in GABA uptake.Key words: GABA, muscimol, nipecotic acid, GABA-uptake blocker, epilepsy.

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