DRUG-INDUCED CHANGES IN THE CONCENTRATION OF 5-OR INDOLYL COMPOUNDS IN CEREBROSPINAL FLUID AND CAUDATE NUCLEUS

Abstract We describe a method for obtaining profiles of neutral and acidic substances present in human cerebrospinal fluid (CSF) by gas—liquid chromatography. Metabolites extracted with ethyl acetate and ether are converted to methyl ester, trimethylsilyl ether derivatives. With this technique, acidic metabolites of brain amines, and neutral metabolites of dopamine and norepinephrine have been identified in human CSF. Some of these substances have not been reported previously in CSF. Extracted substances are identified on the basis of their methylene-unit values and mass spectral data. Potential use of this method in neurodiagnosis and in delineating drug-induced changes in CSF metabolites is discussed.

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Drug Induced Changes in Cell Organelles

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100100299 ◽

1968 ◽

Vol 26 ◽

pp. 110-111

Author(s):

Sarah A. Luse

Keyword(s):

Clinical Medicine ◽

Cell Organelles ◽

Riboflavin Deficiency ◽

Drug Induced ◽

New Era ◽

Health And Disease ◽

In The Beginning ◽

Cellular Pathology ◽

Induced Changes ◽

The Impact

In the mid-nineteenth century Virchow revolutionized pathology by introduction of the concept of “cellular pathology”. Today, a century later, this term has increasing significance in health and disease. We now are in the beginning of a new era in pathology, one which might well be termed “organelle pathology” or “subcellular pathology”. The impact of lysosomal diseases on clinical medicine exemplifies this role of pathology of organelles in elucidation of disease today.Another aspect of cell organelles of prime importance is their pathologic alteration by drugs, toxins, hormones and malnutrition. The sensitivity of cell organelles to minute alterations in their environment offers an accurate evaluation of the site of action of drugs in the study of both function and toxicity. Examples of mitochondrial lesions include the effect of DDD on the adrenal cortex, riboflavin deficiency on liver cells, elevated blood ammonia on the neuron and some 8-aminoquinolines on myocardium.

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Drug-induced changes in the maternal behavior of rats

Psychopharmacology ◽

10.1007/bf00426698 ◽

1977 ◽

Vol 53 (1) ◽

pp. 83-87 ◽

Cited By ~ 18

Author(s):

Slávka Fraňková

Keyword(s):

Maternal Behavior ◽

Drug Induced ◽

Induced Changes ◽

Behavior Of Rats

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Drainage of cerebral interstitial fluid into deep cervical lymph of the rabbit

AJP Renal Physiology ◽

10.1152/ajprenal.1981.240.4.f329 ◽

1981 ◽

Vol 240 (4) ◽

pp. F329-F336 ◽

Cited By ~ 31

Author(s):

M. W. Bradbury ◽

H. F. Cserr ◽

R. J. Westrop

Keyword(s):

Cerebrospinal Fluid ◽

Caudate Nucleus ◽

Subarachnoid Space ◽

Interstitial Fluid ◽

Bulk Flow ◽

Perivascular Spaces ◽

Csf Analysis ◽

Intraventricular Injections ◽

Intracerebral Microinjection ◽

Do So

Lymph from the jugular lymph trunks of anesthetized rabbits has been continuously collected and radioiodinated albumin (RISA) therein estimated after microinjection of 1 microliter of 131I-albumin into the caudate nucleus, after single intraventricular injections, and during intraventricular infusions. Comparison of lymph at 7 and 25 h after intracerebral microinjection with efflux of radioactivity from whole brain suggests that about 50% of cleared radioactivity goes through lymph. Concentrations, normalized to cerebrospinal fluid (CSF), were much higher in lymph and retropharyngeal nodes after brain injection than after CSF injection or infusion. Also after brain injection, lymph and nodes contained more activity on injected side in contrast to lack of laterality after CSF administration. Calculation suggests that less than 30% of RISA cleared from brain can do so via a pool of well-mixed CSF. Analysis of tissues is compatible with much RISA draining by bulk flow via cerebral perivascular spaces plus passage from subarachnoid space of olfactory lobes into submucous spaces of nose and thus to lymph.

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