scholarly journals Production of human papillomavirus type 45 virus-like particles in insect cells using a recombinant baculovirus

1996 ◽  
Vol 141 (1) ◽  
pp. 111-116 ◽  
Author(s):  
Antoine Touzé ◽  
Catherine Dupuy ◽  
Martine Chabaud ◽  
Pierre Le Cann ◽  
Pierre Coursaget
Keyword(s):  
Human Papillomavirus ◽  
Insect Cells ◽  
Recombinant Baculovirus ◽  
Virus Like Particles

scholarly journals Production of SARS-CoV-2 virus-like particles in insect cells

2021 ◽  
Author(s):  
Youjun Mi ◽  
Tao Xie ◽  
Bingdong Zhu ◽  
Jiying Tan ◽  
Xuefeng Li ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Human Health ◽  
Insect Cells ◽  
Vaccine Development ◽  
Recombinant Baculovirus ◽  
Expression Plasmid ◽  
Virus Like Particles ◽  
Recombinant Bacmid ◽  
Multiple Cloning Sites ◽  
S Genes

ABSTRACTCoronavirus disease (COVID-19) causes a serious threat to human health. To production of SARS-COV-2 virus-like particles (VLPs) in insect cells for vaccine development and scientific research. The E, M and S genes were cloned into multiple cloning sites of the new triple expression plasmid with one p10 promoter, two pPH promoters and three multiple cloning sites. The plasmid was transformed into DH10 BacTMEscherichia coli competent cells to obtain recombinant bacmid. Then the recombinant bacmid was transfected in ExpiSf9™ insect cells to generate recombinant baculovirus. After ExpiSf9™ infected with the recombinant baculovirus, the E, M, and S protein co-expressed in insect cells. Finally, SARS-CoV-2 VLPs were self-assembled in insect cells after infection. The morphology and the size of SARS-CoV-2 VLPs are similar to the native virions.

Hepatitis C virus structural proteins and virus-like particles produced in recombinant baculovirus-infected insect cells

2010 ◽  
Vol 44 (1) ◽  
pp. 97-108 ◽  
Author(s):  
S. N. Belzhelarskaya ◽  
N. N. Koroleva ◽  
V. V. Popenko ◽  
V. L. Drutza ◽  
O. V. Orlova ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Insect Cells ◽  
Recombinant Baculovirus ◽  
Structural Proteins ◽  
Virus Like Particles ◽  
Infected Insect

scholarly journals Virus-like particles of potato leafroll virus as potential carrier system for nucleic acids.

2005 ◽  
Vol 52 (3) ◽  
pp. 699-702 ◽  
Author(s):  
Elzbieta Sułuja ◽  
Ludmiła Strokowskaja ◽  
Włodzimierz Zagórski-Ostoja ◽  
Andrzej Pałucha
Keyword(s):  
Coat Protein ◽  
Nucleic Acids ◽  
Coat Protein Gene ◽  
Insect Cells ◽  
Protein Gene ◽  
Recombinant Baculovirus ◽  
Potato Leafroll Virus ◽  
Rnase A ◽  
Virus Like Particles ◽  
Leafroll Virus

Potato leafroll virus is a member of the polerovirus genus. The isometric virion is formed by a coat protein encapsidating single-stranded, positive-sense, mono-partite genomic RNA with covalently attached viral protein at the 5' end. The coat protein of the virus exists in two forms: i) a 23 kDa protein, the product of the coat protein gene, and ii) a 78 kDa protein, the product of the coat protein gene and an additional open reading frame expressed by read-through of the coat protein gene stop codon. The aim of this work was the expression of potato leafroll virus coat protein-based proteins that would be able to assemble into virus-like particles in insect cells. These modified particles were tested for their ability to encapsidate nucleic acids. Two types of N-terminally His-tagged coat protein constructs were used for the expression in insect cells: one, encoding a 23 kDa protein with the C-terminal amino-acid sequence corresponding to the wild type coat protein and the second with additional clathrin binding domain at the C-terminus. The expression of these two proteins by a recombinant baculovirus was characterized by Western immunoblotting with antibodies directed against potato leafroll virus. The protection or putative encapsidation of nucleic acids by these two coat protein derivatives was shown by DNase I and RNase A protection assays.

Assembly and release of HIV-1 precursor Pr55gag virus-like particles from recombinant baculovirus-infected insect cells

Cell ◽  
1989 ◽  
Vol 59 (1) ◽  
pp. 103-112 ◽  
Author(s):  
Dirk Gheysen ◽  
Eric Jacobs ◽  
Françoise de Foresta ◽  
Clotilde Thiriart ◽  
Myriam Francotte ◽  
...  
Keyword(s):  
Insect Cells ◽  
Recombinant Baculovirus ◽  
Virus Like Particles ◽  
Infected Insect ◽  
Hiv 1

scholarly journals Production of SARS-CoV-2 Virus-Like Particles in Insect Cells

Vaccines ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 554
Author(s):  
Youjun Mi ◽  
Tao Xie ◽  
Bingdong Zhu ◽  
Jiying Tan ◽  
Xuefeng Li ◽  
...  
Keyword(s):  
Human Health ◽  
Insect Cells ◽  
Vaccine Development ◽  
Recombinant Baculovirus ◽  
Expression Plasmid ◽  
Virus Like Particles ◽  
Competent Cells ◽  
Recombinant Bacmid ◽  
Multiple Cloning Sites ◽  
S Genes

Coronavirus disease (COVID-19) causes a serious threat to human health. Virus-like particles (VLPs) constitute a promising platform in SARS-CoV-2 vaccine development. In this study, the E, M, and S genes were cloned into multiple cloning sites of a new triple expression plasmid with one p10 promoter, two pPH promoters, and three multiple cloning sites. The plasmid was transformed into DH10 BacTMEscherichia coli competent cells to obtain recombinant bacmid. Then the recombinant bacmid was transfected in ExpiSf9TM insect cells to generate recombinant baculovirus. After ExpiSf9TM cells infection with the recombinant baculovirus, the E, M, and S proteins were expressed in insect cells. Finally, SARS-CoV-2 VLPs were self-assembled in insect cells after infection. The morphology and the size of SARS-CoV-2 VLPs are similar to the native virions.

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