scholarly journals Crude oil degradation efficiency of a recombinantAcinetobacter baumanniistrain and its survival in crude oil-contaminated soil microcosm

2004 ◽  
Vol 235 (2) ◽  
pp. 323-331 ◽  
Author(s):  
Sanjeet Mishra ◽  
Priyangshu M Sarma ◽  
Banwari Lal
Keyword(s):  
Crude Oil ◽  
Contaminated Soil ◽  
Soil Microcosm ◽  
Degradation Efficiency ◽  
Oil Degradation ◽  
Crude Oil Degradation

Performance of some New Bacterial Isolates on Biodegradation of Libyan Light Crude Oil Using Agro-Industrial Wastes as Co-Substrates

2015 ◽  
Vol 802 ◽  
pp. 496-500 ◽  
Author(s):  
Abdullah M. El Mahdi ◽  
Hamidi Abdul Aziz ◽  
Salem S. Abu Amr ◽  
Nour Sh. El-Gendy ◽  
Hussein Nassar
Keyword(s):  
Crude Oil ◽  
Corn Steep Liquor ◽  
Degradation Efficiency ◽  
Industrial Wastes ◽  
Hydrocarbon Degradation ◽  
Environmental Damage ◽  
Rrna Gene ◽  
Oil Degradation ◽  
Bacterial Isolates ◽  
Crude Oil Degradation

Some unfortunate accidents of large amount of oil hydrocarbons have been reported in Mediterranean Sea which has caused severe environmental damage to the area. To safeguard the Libyan coastline and marine ecologysince thousands of human activity such as fishing largely depends on the healthycoastal environment.Twenty crude oil-degrading bacterial isolates were obtained from oil-contaminated sites at Al Hariga Oil Terminal and Nafoora Oilfield. Based on a high growth rate in crude oil and hydrocarbon degradation efficiency, two isolates were selected from the twenty isolates for further analysis. The nucleotide sequence of 16S rRNA gene showed that these isolates are likely Pseudomonas aeruginosa andKocuriapastrius. One of the isolates is a potential Gram-negative Pseudomonas bacterium based on petroleum hydrocarbon degradation efficiency and potent emulsifying activity; thus, this isolate is identified as P. aeruginosa NAF1; the other isolate K. pastrius SAR3 is identified as a Gram-positive bacterium. Corn steep liquor (CSL) and solid waste date (SWD) were used for an in situ molasses technique to enhance bacterial growth and biodegradation efficiency. P. aeruginosa NAF1 exhibited 70% and 76% crude oil degradation in 0.2% (w/v) CSL and SWD in 28 d, respectively. Likewise, K. pastrius SAR3 yielded 68% and 70% crude oil degradation in 0.2% (w/v) CSL and SWD in 28 d, respectively.

scholarly journals The potential of yeasts from the oil-contaminated soil and mangrove rhizosphere for degrading crude oil

2019 ◽  
Vol 20 (7) ◽  
Author(s):  
NUR HIDAYATUL ALAMI ◽  
LUTHFI LAILATUL MAULIDIYA ◽  
N D KUSWYTASARI ◽  
ENNY ZULAIKA ◽  
MAYA SHOVITRI
Keyword(s):  
Soil Moisture ◽  
Crude Oil ◽  
Contaminated Soil ◽  
Statistical Tests ◽  
Gravimetric Method ◽  
Plate Count ◽  
Microbial Culture ◽  
Oil Degradation ◽  
Degradation Rates ◽  
Crude Oil Degradation

Abstract. Alami NH, Maulidiya LL, Kuswytasari ND, Zulaika E, Shovitri M. 2019. The potential of yeasts from the oil-contaminated soil and mangrove rhizosphere for degrading crude oil. Biodiversitas 20: 1833-1838. Bioremediation of crude oil contaminated soil eventually depends on the activities of microorganisms to degrade contaminants. Bioaugmentation, through the addition of microbial culture to contaminated soil, can be used to enhance degradation rates. This research aimed to determine the effect of yeast formulations isolated from the oil-contaminated soil and mangrove rhizosphere to degrade crude oil and evaluate the most effective yeast formulation and incubation timefor crude oil degradation. This research was conducted by subculturing yeast in the slant agar medium, screening hydrocarbonoclastic yeast in crude oil- Bushnell Hass Mineral Salt (BHMS) medium, biodegradation test, measuring the total cell number with Total Plate Count (TPC), Total Petroleum Hydrocarbon (TPH) test with the gravimetric method, and measuring soil moisture content. The design of the research was Completely Randomized Factorial Design with 3 replications. Data were analyzed quantitatively by using statistical tests for TPC and TPH, while a descriptive qualitative test analyzed soil moisture. The result showed that yeast formulation and incubation time affected crude oil degradation. An effective yeast consortium for oil degradation is T20 consortium with an incubation period of 30 days, which result in 72.26 % oil degradation.

Methanogenic crude oil degradation induced by an exogenous microbial community and nutrient injections

2021 ◽  
Vol 201 ◽  
pp. 108458
Author(s):  
Konomi Suda ◽  
Masayuki Ikarashi ◽  
Hideyuki Tamaki ◽  
Satoshi Tamazawa ◽  
Susumu Sakata ◽  
...  
Keyword(s):  
Microbial Community ◽  
Crude Oil ◽  
Oil Degradation ◽  
Crude Oil Degradation

Comparison of surficial CO2 efflux to other measures of subsurface crude oil degradation

2014 ◽  
Vol 164 ◽  
pp. 275-284 ◽  
Author(s):  
Ean Warren ◽  
Natasha J. Sihota ◽  
Frances D. Hostettler ◽  
Barbara A. Bekins
Keyword(s):  
Crude Oil ◽  
Co2 Efflux ◽  
Oil Degradation ◽  
Crude Oil Degradation

scholarly journals Efficiency and Kinetics of Assam Crude Oil Degradation by Pseudomonas Aeruginosa AKS1 and Bacillus Sp. AKS2

2021 ◽  
Author(s):  
Bobby Chettri ◽  
Ningombam Anjana Singha ◽  
Arvind Kumar Singh
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Crude Oil ◽  
Liquid Medium ◽  
Half Life ◽  
Liquid Culture ◽  
Oil Degradation ◽  
Biodegradation Rate ◽  
Emulsification Index ◽  
Crude Oil Degradation ◽  
Kinetics Of

Abstract We report kinetics of Assam crude oil degradation by Pseudomonas aeruginosa AKS1 and Bacillus sp. AKS2, both isolated from Assam refinery sediments. The isolates exhibited appreciable degrees of hydrophobicity, emulsification index and biosurfactant production. Crude oil degradation efficiency of isolates was assessed in (1) liquid medium amended with 1% v/v crude oil and (2) microcosm sediments (125 mg crude oil/ 10 g sand). In liquid culture, the biodegradation rate (k) and half-life (t1/2) values were found to be 0.0383 day -1 and 18.09 days for P. aeruginosa AKS1, and 0.0204 day -1 and 33.97 days in case of Bacillus sp. AKS2. In microcosm sand sediments, the estimated biodegradation rate (k) and half-life (t 1/2) values were 0.0138 day -1 and 50 days for P. aeruginosa AKS1, and 0.0113 day -1 and 61.34 days in case of Bacillus sp. AKS2. The level of nutrient treatment in microcosm sand sediment was 125 µg N & 62.5 µg P/g sediment in case of P. aeruginosa AKS1 and 375 µg N & 37.5 µg P/g sediment in case of Bacillus sp. AKS2. In microcosms without inorganic nutrients, biodegradation rate (k) and half-life (t1/2) values were found to be 0.0069 day -1 and 100 days for P. aeruginosa AKS1 and for Bacillus sp. AKS2, the respective values were found to be 0.0046 day -1 and 150.68 days. Our data provides important information for predictive hydrocarbon degradation in liquid medium and contaminated sediments.

Kinetics of nutrient enhanced crude oil degradation by Pseudomonas aeruginosa AKS1 and Bacillus sp. AKS2 isolated from Guwahati refinery, India

2016 ◽  
Vol 216 ◽  
pp. 548-558 ◽  
Author(s):  
Bobby Chettri ◽  
Arghya Mukherjee ◽  
James S. Langpoklakpam ◽  
Dhrubajyoti Chattopadhyay ◽  
Arvind K. Singh
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Crude Oil ◽  
Oil Degradation ◽  
Bacillus Sp ◽  
Crude Oil Degradation ◽  
Kinetics Of
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