0360 - Genomic analysis provides insights into extreme environmental adaptation and crude oil degradation by a new polyextremophile Halomonas desertis G11

2018 ◽  
Author(s):  
Mohamed Neifar ◽  
Habib CHOUCHANE
Keyword(s):  
Crude Oil ◽  
Genomic Analysis ◽  
Environmental Adaptation ◽  
Oil Degradation ◽  
Crude Oil Degradation

Methanogenic crude oil degradation induced by an exogenous microbial community and nutrient injections

2021 ◽  
Vol 201 ◽  
pp. 108458
Author(s):  
Konomi Suda ◽  
Masayuki Ikarashi ◽  
Hideyuki Tamaki ◽  
Satoshi Tamazawa ◽  
Susumu Sakata ◽  
...  
Keyword(s):  
Microbial Community ◽  
Crude Oil ◽  
Oil Degradation ◽  
Crude Oil Degradation

Comparison of surficial CO2 efflux to other measures of subsurface crude oil degradation

2014 ◽  
Vol 164 ◽  
pp. 275-284 ◽  
Author(s):  
Ean Warren ◽  
Natasha J. Sihota ◽  
Frances D. Hostettler ◽  
Barbara A. Bekins
Keyword(s):  
Crude Oil ◽  
Co2 Efflux ◽  
Oil Degradation ◽  
Crude Oil Degradation

scholarly journals Efficiency and Kinetics of Assam Crude Oil Degradation by Pseudomonas Aeruginosa AKS1 and Bacillus Sp. AKS2

2021 ◽  
Author(s):  
Bobby Chettri ◽  
Ningombam Anjana Singha ◽  
Arvind Kumar Singh
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Crude Oil ◽  
Liquid Medium ◽  
Half Life ◽  
Liquid Culture ◽  
Oil Degradation ◽  
Biodegradation Rate ◽  
Emulsification Index ◽  
Crude Oil Degradation ◽  
Kinetics Of

Abstract We report kinetics of Assam crude oil degradation by Pseudomonas aeruginosa AKS1 and Bacillus sp. AKS2, both isolated from Assam refinery sediments. The isolates exhibited appreciable degrees of hydrophobicity, emulsification index and biosurfactant production. Crude oil degradation efficiency of isolates was assessed in (1) liquid medium amended with 1% v/v crude oil and (2) microcosm sediments (125 mg crude oil/ 10 g sand). In liquid culture, the biodegradation rate (k) and half-life (t1/2) values were found to be 0.0383 day -1 and 18.09 days for P. aeruginosa AKS1, and 0.0204 day -1 and 33.97 days in case of Bacillus sp. AKS2. In microcosm sand sediments, the estimated biodegradation rate (k) and half-life (t 1/2) values were 0.0138 day -1 and 50 days for P. aeruginosa AKS1, and 0.0113 day -1 and 61.34 days in case of Bacillus sp. AKS2. The level of nutrient treatment in microcosm sand sediment was 125 µg N & 62.5 µg P/g sediment in case of P. aeruginosa AKS1 and 375 µg N & 37.5 µg P/g sediment in case of Bacillus sp. AKS2. In microcosms without inorganic nutrients, biodegradation rate (k) and half-life (t1/2) values were found to be 0.0069 day -1 and 100 days for P. aeruginosa AKS1 and for Bacillus sp. AKS2, the respective values were found to be 0.0046 day -1 and 150.68 days. Our data provides important information for predictive hydrocarbon degradation in liquid medium and contaminated sediments.

Kinetics of nutrient enhanced crude oil degradation by Pseudomonas aeruginosa AKS1 and Bacillus sp. AKS2 isolated from Guwahati refinery, India

2016 ◽  
Vol 216 ◽  
pp. 548-558 ◽  
Author(s):  
Bobby Chettri ◽  
Arghya Mukherjee ◽  
James S. Langpoklakpam ◽  
Dhrubajyoti Chattopadhyay ◽  
Arvind K. Singh
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Crude Oil ◽  
Oil Degradation ◽  
Bacillus Sp ◽  
Crude Oil Degradation ◽  
Kinetics Of

Investigation of crude oil degradation using metal oxide anode-based microbial fuel cell

2020 ◽  
Vol 11 ◽  
pp. 100449
Author(s):  
Arpita Nandy ◽  
Jagoš R. Radović ◽  
Breda Novotnik ◽  
Mohita Sharma ◽  
Stephen R. Larter ◽  
...  
Keyword(s):  
Fuel Cell ◽  
Metal Oxide ◽  
Crude Oil ◽  
Microbial Fuel Cell ◽  
Oil Degradation ◽  
Crude Oil Degradation ◽  
Metal Oxide Anode ◽  
Oxide Anode

scholarly journals Newly Isolated Alkane Hydroxylase and Lipase Producing Geobacillus and Anoxybacillus Species Involved in Crude Oil Degradation

Catalysts ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 851 ◽  
Author(s):  
Durratul Fatini Yusoff ◽  
Raja Noor Zaliha Raja Abd Rahman ◽  
Malihe Masomian ◽  
Mohd Shukuri Mohamad Ali ◽  
Thean Chor Leow
Keyword(s):  
Crude Oil ◽  
Oil Spills ◽  
Oil Degradation ◽  
Alkane Hydroxylase ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
Crude Oil Degradation ◽  
16S Rrna Sequence Analysis ◽  
First Time ◽  
Poly Aromatic Hydrocarbons

Isolation and studies of novel, crude oil biodegrading thermophilic strains may provide a wider knowledge in understanding their role in petroleum degradation. In this study, the screening of ten new thermophilic strains revealed that all strains were alkane hydroxylase producers and seven of them produced lipase concurrently. Three best strains were characterized and identified through 16S rRNA sequence analysis as Geobacillus sp. D4, Geobacillus sp. D7, and Anoxybacillus geothermalis D9 with GenBank accession numbers MK615934.1, MK615935.1, and MK615936.1, respectively. Gas chromatography (GC) analysis showed that all three strains were able to breakdown various compounds in crude oil such as alkanes, toxic poly-aromatic hydrocarbons (PAHs), organosulfur, carboxylic acids, alkene, resins, organosilicon, alcohol, organochlorine, and ester. For the first time, alkane hydroxylase and lipase activity as well as crude oil degradation by A. geothermalis species were reported. Geobacillus sp. D7 is the best alkane degrader followed by A. geothermalis D9 and Geobacillus sp. D4 with 17.3%, 13.1%, and 12.1% biodegradation efficiency (BE%), respectively. The potential of thermophiles isolated can be explored further for bioremediation of sites polluted by petroleum and oil spills.

scholarly journals Crude Oil Degrading Fingerprint and the Overexpression of Oxidase and Invasive Genes for n-hexadecane and Crude Oil Degradation in the Acinetobacter pittii H9-3 Strain

2019 ◽  
Vol 16 (2) ◽  
pp. 188 ◽  
Author(s):  
Yang Wang ◽  
Qiuyu Wang ◽  
Limei Liu
Keyword(s):  
Crude Oil ◽  
Carbon Sources ◽  
Morphological Characteristics ◽  
Oil Degradation ◽  
16S Rdna Sequence Analysis ◽  
Degrading Bacterium ◽  
Branched Alkanes ◽  
Crude Oil Degradation ◽  
Acinetobacter Pittii ◽  
The Difference

A crude oil-degrading bacterium named strain H9-3 was isolated from crude oil contaminated soil in the Northeastern area of China. Based on its morphological characteristics and 16S rDNA sequence analysis, strain H9-3 is affiliated to Acinetobacter pittii in the group of Gammaproteobacteria. The strain was efficient in removing 36.8% of the initial 10 g·L - 1 of crude oil within 21 days. GC-MS was performed and a preference was shown for n-C10, n-C11, i-C14, i-C17, i-C34, n-C12, n-C13, n-C14, n-C27, n-C32 and i-C13, over n-C16, n-C18–C22, n-C24–n-C31, and n-C36. This can be regarded as the specific fingerprint for crude oil degradation by strain H9-3 of Acinetobacter pittii. In addition to crude oil, it was shown that soybean oil and phenols can be utilized as carbon sources by strain H9-3. It was also shown that aniline and α -naphthol cannot be utilized for growth, but they can be tolerated by strain H9-3. Methylbenzene was neither utilized nor tolerated by strain H9-3. Although n-hexadecane was not preferentially consumed by strain H9-3, during culture with crude oil, it could be utilized for growth when it is the sole carbon source. The degradation of some branched alkanes (i-C14, i-C17 and i-C34) and the preferential degradation of crude oil over phenols could be used as a reference for distinguishing A. pittii from A. calcoaceticus. The difference in gene expression was very significant and was induced by diverse carbon sources, as shown in the qRT-PCR results. The oxidation and adhesion events occurred at high frequency during alkane degration by Acinetobacter pittii strain H9-3 cells.

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