FINDINGS IN ROUTINE LABORATORY EXAMINATION IN PROGRESSIVE MYOCLONUS EPILEPSY

After the observation of many thousands of histological sections of the endocervical mucosa it became evident that its columnar cells present a great variety of aspects not only those of the surface of the canal but also those of the glands. A classification of these cells was made taking into account the staining affinity, the intensity staining of the cytoplasm, the presence or absence of cilia, the shape and location of the nucleus. The various combinations of these different data made possible the characterization of 26 types of cells which we labelled by the alphabetical letters. Two hundred and fifty cervices obtained by cervical amputation and by hysterectomy were studied. The uteri presented lesions in the course of routine laboratory examination. In each of the 250 histological sections there were specifically counted 2,000 columnar cells which cover the cervical canal and 2,000columnar cells which form the glands. A graphic representation of the frequency of both the superficial and glandular columnar cells was presented; this was given the name EPITHELIOGRAM. The variation of the cellular "composition" of each epithelium is discussed and the frequency of the various cellular types after the count of one million of cells is presented.

Download Full-text

Antimicrobial Susceptibility Testing ofBilophila wadsworthia Isolates Submitted for Routine Laboratory Examination

Journal of Clinical Microbiology ◽

10.1128/jcm.36.6.1790-1792.1998 ◽

1998 ◽

Vol 36 (6) ◽

pp. 1790-1792 ◽

Cited By ~ 3

Author(s):

Chikako Mochida ◽

Yoichi Hirakata ◽

Junichi Matsuda ◽

Fumiaki Iori ◽

Yumi Ozaki ◽

...

Keyword(s):

Antimicrobial Susceptibility ◽

Pyruvic Acid ◽

Measurement Method ◽

Susceptibility Testing ◽

Inoculum Size ◽

Routine Laboratory ◽

Broth Microdilution ◽

Laboratory Examination ◽

Routine Laboratory Examination ◽

Agar Dilution

MICs of antibiotics against Bilophila wadsworthiaisolates were measured by agar and broth microdilution with pyruvic acid and by Etest. The inoculum size influenced greatly agar dilution. Despite discrepancies in MICs depending on the measurement method used, clindamycin consistently showed potent activity. Broth microdilution and Etest appear to be candidates for laboratory susceptibility testing.

Download Full-text

An Enquiry into the Coli anaerogenes Bacteria

Journal of Hygiene ◽

10.1017/s0022172400034392 ◽

1934 ◽

Vol 34 (1) ◽

pp. 69-80 ◽

Cited By ~ 7

Author(s):

J. Bamforth

Keyword(s):

The Other ◽

Routine Laboratory ◽

Laboratory Examination ◽

Gram Negative ◽

Group A ◽

Routine Laboratory Examination ◽

Ferment Lactose ◽

Different Strains

In the routine laboratory examination of specimens of faeces and urine, from time to time certain Gram-negative bacilli belonging to many different groups are encountered, which morphologically show no appreciable differences from the Gram-negative bacilli of the coli-typhoid group, but which however ferment the usual carbohydrate media employed in differentiation with the formation of acid only. Some ferment lactose quickly, others more slowly, and many not at all, but as with the other media no gas is formed. These organisms might be considered as belonging to the so-called B. coli anaerogenes group— a somewhat loose term which might include many different strains, some perhaps related to the colon group, others belonging to and related to the group of dysentery bacilli, and others showing no relationship to either.

Download Full-text

Progressive myoclonus epilepsy: a differential diagnosis from juvenile chorea Huntington disease

Neuropediatrics ◽

10.1055/s-0033-1337705 ◽

2013 ◽

Vol 44 (02) ◽

Author(s):

M Gosk ◽

F Laccone ◽

P Burfeind ◽

G Bernert

Keyword(s):

Differential Diagnosis ◽

Huntington Disease ◽

Chorea Huntington ◽

Progressive Myoclonus Epilepsy ◽

Myoclonus Epilepsy

Download Full-text

Altered Expression of Peroxiredoxins in Mouse Model of Progressive Myoclonus Epilepsy upon LPS-Induced Neuroinflammation

Antioxidants ◽

10.3390/antiox10030357 ◽

2021 ◽

Vol 10 (3) ◽

pp. 357

Author(s):

Mojca Trstenjak Prebanda ◽

Petra Matjan-Štefin ◽

Boris Turk ◽

Nataša Kopitar-Jerala

Keyword(s):

Mouse Model ◽

Redox Homeostasis ◽

Underlying Mechanism ◽

Loss Of Function ◽

Altered Expression ◽

Lps Challenge ◽

Progressive Myoclonus Epilepsy ◽

Myoclonus Epilepsy ◽

The Brain ◽

Deficient Mice

Stefin B (cystatin B) is an inhibitor of endo-lysosomal cysteine cathepsin, and the loss-of-function mutations in the stefin B gene were reported in patients with Unverricht–Lundborg disease (EPM1), a form of progressive myoclonus epilepsy. Stefin B-deficient mice, a mouse model of the disease, display key features of EPM1, including myoclonic seizures. Although the underlying mechanism is not yet completely clear, it was reported that the impaired redox homeostasis and inflammation in the brain contribute to the progression of the disease. In the present study, we investigated if lipopolysaccharide (LPS)-triggered neuroinflammation affected the protein levels of redox-sensitive proteins: thioredoxin (Trx1), thioredoxin reductase (TrxR), peroxiredoxins (Prxs) in brain and cerebella of stefin B-deficient mice. LPS challenge was found to result in a marked elevation of Trx1 and TrxR in the brain and cerebella of stefin B deficient mice, while Prx1 was upregulated only in cerebella after LPS challenge. Mitochondrial peroxiredoxin 3 (Prx3), was upregulated also in the cerebellar tissue lysates prepared from unchallenged stefin B deficient mice, while after LPS challenge Prx3 was upregulated in stefin B deficient brain and cerebella. Our results imply the role of oxidative stress in the progression of the disease.

Download Full-text