A high-affinity folate binding protein in human cerebrospinal fluid

2009 ◽  
Vol 71 (2) ◽  
pp. 133-135 ◽  
Author(s):  
Steen Ingemann Hansen ◽  
Jan Holm ◽  
Jørgen Lyngbye
Keyword(s):  
Cerebrospinal Fluid ◽  
Binding Protein ◽  
Folate Binding Protein ◽  
High Affinity ◽  
Human Cerebrospinal Fluid

Quantitation of the Folate Binding Protein in Human Cerebrospinal Fluid by an Enzyme-linked Immunosorbent Assay (ELISA)

Pteridines ◽  
1989 ◽  
Vol 1 (4) ◽  
pp. 231-233 ◽  
Author(s):  
Steen Ingemann Hansen ◽  
Jan Holm ◽  
Mimi Høier-Madsen
Keyword(s):  
Cerebrospinal Fluid ◽  
Immunosorbent Assay ◽  
Coefficient Of Variation ◽  
Binding Protein ◽  
Enzyme Linked Immunosorbent Assay ◽  
Major Peak ◽  
Folate Binding Protein ◽  
Human Cerebrospinal Fluid ◽  
Minor Peak

Summary An enzyme-linked immunosorbent assay (ELISA) was calibrated and used for quantitation of the folate binding protein in human cerebrospinal fluid. The precision of the assay in terms of the coefficient of variation was 6.3% intra-assay (10 replicate single determinations) and 4.8% inter-assay (8 duplicate determinations). The concentration of folate binding protein in cerebrospinal fluid specimens from 20 patients with no complaints or diseases of neurological type was 0.14-0.38, 0.24 nmol FBP/I (range and median value). The gelfiltration profiles of radioligand bound and immunoreactive folate binding protein were identical, both consisting of one major peak (M, '" 25000) and one minor peak (M, '" 1 00000).

A high affinity folate binding protein in umbilical cord serum

1980 ◽  
Vol 40 (6) ◽  
pp. 523-527 ◽  
Author(s):  
Jan Holm ◽  
Steen Ingemann Hansen ◽  
Jörgen Lyngbye
Keyword(s):  
Umbilical Cord ◽  
Binding Protein ◽  
Folate Binding Protein ◽  
Cord Serum ◽  
High Affinity

scholarly journals Isolation from human cerebrospinal fluid of a new insulin-like growth factor-binding protein with a selective affinity for IGF-II

FEBS Letters ◽  
1989 ◽  
Vol 255 (2) ◽  
pp. 253-258 ◽  
Author(s):  
Monireh Roghani ◽  
Paul Hossenlopp ◽  
Pierre Lepage ◽  
Alain Balland ◽  
Michel Binoux
Keyword(s):  
Cerebrospinal Fluid ◽  
Growth Factor ◽  
Binding Protein ◽  
Insulin Like Growth Factor ◽  
Factor Binding ◽  
Human Cerebrospinal Fluid

High-affinity folate binding in human prostate

1993 ◽  
Vol 13 (2) ◽  
pp. 99-105 ◽  
Author(s):  
Jan Holm ◽  
Steen Ingemann Hansen ◽  
Mimi Høier-Madsen
Keyword(s):  
Human Milk ◽  
Binding Protein ◽  
Cross Reactivity ◽  
Human Prostate ◽  
Enzyme Linked Immunosorbent Assay ◽  
Gel Chromatography ◽  
Folate Binding Protein ◽  
High Affinity ◽  
Sds Page ◽  
Triton X

Binding of 3H-folate in Triton X-100 solubilized human prostate homogenate was of a high-affinity type and displayed apparent positive cooperativity typical of specific folate binding. Radioligand dissociation was slow at pH 7.4, but rapid at pH 3.5. Gel chromatography reveled two major folate binding proteins (Mr≈100 and 25kDa), but only one single band (Mr ≈ 65–70 kDa) was detectable on SDS-PAGE and immunoblotting with rabbit-anti human milk folate binding protein. Concentration of folate binding protein in prostate homogenate expressed as maximum 3H-folate binding was 1.10 nmol/g protein, and the cross-reactivity with rabbit-anti human milk folate binding protein serum was 15% as determined by an enzyme-linked immunosorbent assay (median values; n = 6).

A high-affinity folate binding protein in human urine. Radioligand binding characteristics, immunological properties and molecular size

1989 ◽  
Vol 9 (1) ◽  
pp. 93-97 ◽  
Author(s):  
Steen Ingemann Hansen ◽  
Jan Holm ◽  
Mimi Høier-Madsen
Keyword(s):  
Human Urine ◽  
Radioligand Binding ◽  
Binding Protein ◽  
Molecular Size ◽  
Enzyme Linked Immunosorbent Assay ◽  
Folate Binding Protein ◽  
Binding Characteristics ◽  
High Affinity ◽  
Large Peak ◽  
Apparently Healthy

High-affinity binding of [3H]folate in human urine displayed characteristics, e.g. apparent positive cooperativity, which are typical of specific folate binding. By means of a two-site enzyme-linked immunosorbent assay (ELISA) with rabbit antibodies against the low molecular weight folate binding protein from human milk, we measured folate binding protein concentrations in the range of 0.51 to 4.13 nM in urine samples from 16 apparently healthy individuals. Ultrogel AcA 44 chromatography of the urine showed that immunoreactive and radioligand bound folate binding protein coeluted in one large peak (Mr∼25,000).

scholarly journals Folate deficiency in cerebrospinal fluid associated with a defect in folate binding protein in the central nervous system.

1994 ◽  
Vol 57 (2) ◽  
pp. 223-226 ◽  
Author(s):  
R A Wevers ◽  
S I Hansen ◽  
J L van Hellenberg Hubar ◽  
J Holm ◽  
M Hoier-Madsen ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Cerebrospinal Fluid ◽  
Nervous System ◽  
Binding Protein ◽  
Folate Deficiency ◽  
Folate Binding Protein ◽  
The Central Nervous System

Identification of an insulin-like growth factor-binding protein in human cerebrospinal fluid with a selective affinity for IGF-II

FEBS Letters ◽  
1986 ◽  
Vol 208 (2) ◽  
pp. 439-444 ◽  
Author(s):  
Paul Hossenlopp ◽  
Danielle Seurin ◽  
Berta Segovia-Quinson ◽  
Michel Binoux
Keyword(s):  
Cerebrospinal Fluid ◽  
Growth Factor ◽  
Binding Protein ◽  
Insulin Like Growth Factor ◽  
Factor Binding ◽  
Human Cerebrospinal Fluid

A high-affinity folate binding protein in normal human leukocytes: Ligand binding characteristics, ionic charge and molecular size

1985 ◽  
Vol 5 (8) ◽  
pp. 683-688 ◽  
Author(s):  
Jan Holm ◽  
Steen Ingemann Hansen ◽  
J⊘rgen Lyngbye
Keyword(s):  
Binding Protein ◽  
Molecular Size ◽  
Binding Activity ◽  
Ionic Charge ◽  
Folate Binding Protein ◽  
Human Leukocytes ◽  
Binding Characteristics ◽  
High Affinity ◽  
Chromatographic Profile ◽  
Triton X

High-affinity binding of [3H]folate to supernatant from homogenized human leukocytes containing large amounts of binding protein displayed apparent positive cooperativity. The DEAE-Sepharose® CL-6B chromatographic profile of the supernatant at pH 6.3 contained a major peak of folate binding (Mr approx. 25 000) in the front effluent and a smaller more acidic peak (Mr approx. 25 000) that emerged after a rise in NaCl from 30 mmol/l to 1 mol/l. Triton X-100 solubilized ceil sediment from the leukocyte homogenate contained some high-affinity folate binding activity (Mr approx 25 000), typically 5–10% of the total binding activity.

Characterization of a High-Affinity Folate Receptor in Normal and Malignant Human Testicular Tissue

1999 ◽  
Vol 19 (6) ◽  
pp. 571-580 ◽  
Author(s):  
Jan Holm ◽  
Steen Ingemann Hansen ◽  
Mimi Høier-Madsen ◽  
Thomas Broe Christensen ◽  
Carl W. Nichols
Keyword(s):  
Human Milk ◽  
G Protein ◽  
Radioligand Binding ◽  
Binding Protein ◽  
Folate Receptor ◽  
Testicular Tissue ◽  
Hydrophobic Membrane ◽  
Folate Binding Protein ◽  
High Affinity ◽  
Triton X

We have characterized the folate receptor in normal and malignant tissue from male gonads. Radioligand binding displayed characteristics typical of other folate receptors. Those included a high-affinity type of binding (K = 1010 M−1), apparent positive cooperativity changing into non-cooperativity at low receptor concentrations, a tendency to increased binding affinity with decreasing receptor concentrations, a slow dissociation at pH 7.4 becoming rapid at pH 3.5 and inhibition by folates, in particular oxidized forms. The gel filtration profile of Triton X-100 solubilized tissue contained a 25 and 100 kDa peak of radioligand-receptor. The latter peak could represent receptor equipped with a hydrophobic membrane anchor that inserts into Triton X-100 micelles. The concentration of radiolabelled receptor ranged from 0.41 nmol/g protein to 1.68 nmol/g protein in specimens of normal testicular tissue from patients with prostatic carcinomas and from 1.54 nmol/g protein to 3.82 nmol/g protein in testicular tissue from young individuals. Compared to normal testicular tissue the concentration of receptor in seminoma tissue was low (0.38–1.27 nmol/g protein) but showed a higher degree of immunoreactivity in the presence of antibodies against human milk folate binding protein as evidenced by ELISA and immunohistochemistry data. Hence a folate receptor isoform homologous to human milk folate binding protein is apparently expressed in seminomas where the total expression of receptor, however, seems to be lower than in normal testicles.

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