Prolongation of medium exchange is associated with a decrease in function but not expression of the P-glycoprotein pump in leukaemic cells

2009 ◽  
Vol 56 (1-2) ◽  
pp. 12-22 ◽  
Author(s):  
S. Hegewisch-Becker ◽  
C. Faltz ◽  
D. K. Hossfeld
Keyword(s):  
P Glycoprotein ◽  
Medium Exchange ◽  
Leukaemic Cells

Escape from stress granule sequestration: another way to drug resistance?

2010 ◽  
Vol 38 (6) ◽  
pp. 1537-1542 ◽  
Author(s):  
Ernesto Yagüe ◽  
Selina Raguz
Keyword(s):  
Endoplasmic Reticulum ◽  
Multidrug Resistance ◽  
Stress Granules ◽  
Stress Granule ◽  
Chemotherapeutic Drugs ◽  
P Glycoprotein ◽  
Mdr1 Mrna ◽  
Leukaemic Cells ◽  
Multidrug Resistance 1 Gene ◽  
Polysome Profile

Overexpression of P-glycoprotein, encoded by the MDR1 (multidrug resistance 1) gene, is often responsible for multidrug resistance and chemotherapy failure in cancer. We have demonstrated that, in leukaemic cells, P-glycoprotein expression is regulated at the translational level. More recently, we have shown that in cells overexpressing P-glycoprotein, MDR1 mRNA does not aggregate into translationally silent stress granules. Importantly, this is not unique for MDR1, since other transcripts encoding transmembrane proteins, and which are thus translated at the endoplasmic reticulum, follow the same pattern. By using a series of chimaeric transcripts, we have demonstrated that transcript localization at the endoplasmic reticulum bypasses the signals dictating stress granule sequestration. Polysome profile analyses and protein synthesis experiments indicate that, upon stress withdrawal, endoplasmic-reticulum-bound transcripts resume translation faster than those at the cytosol, which have been sequestered into stress granules. This may represent a novel mechanism by which drug-resistant cells respond quickly to stress, helping them to survive the cytotoxic effect of chemotherapeutic drugs.

scholarly journals Reversal of Human Multi-Drug Resistance Leukaemic Cells by Stemofoline Derivatives via Inhibition of P-Glycoprotein Function

2014 ◽  
Vol 116 (5) ◽  
pp. 390-397 ◽  
Author(s):  
Sonthaya Umsumarng ◽  
Pornsiri Pitchakarn ◽  
Kwankamol Sastraruji ◽  
Supachai Yodkeeree ◽  
Alison T. Ung ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Multi Drug Resistance ◽  
P Glycoprotein ◽  
Leukaemic Cells

High Functional P-glycoprotein Activity is More Often Present in T-cell Acute Lymphoblastic Leukaemic Cells in Adults than in Children

2003 ◽  
Vol 44 (1) ◽  
pp. 85-95 ◽  
Author(s):  
Sabine L.A. Plasschaert ◽  
Edo Vellenga ◽  
Eveline S.J.M. de Bont ◽  
Dorina M. van der Kolk ◽  
Anjo J.P. Veerman ◽  
...  
Keyword(s):  
T Cell ◽  
P Glycoprotein ◽  
Leukaemic Cells

Myeloid leukaemic cells can lyse fibrin directly

2000 ◽  
Vol 111 (2) ◽  
pp. 524-529 ◽  
Author(s):  
Linda Robbie ◽  
Susan Berry ◽  
Elaine Moir ◽  
Nuala A. Booth ◽  
Dominic Culligan ◽  
...  
Keyword(s):  
Leukaemic Cells

PREFERENTIAL ELIMINATION OF LEUKAEMIC CELLS BY PORPHYRIN-INDUCED LASER PHOTOSENSITIZATION

1987 ◽  
Vol 48 (C7) ◽  
pp. C7-297-C7-297
Author(s):  
T. AHMED ◽  
S. GINGRICH ◽  
R. DREYFUS ◽  
Z. ARLIN ◽  
J. D. LUTTON ◽  
...  
Keyword(s):  
Leukaemic Cells

Pharmacogenetics of Clozapine: In Vivo Role of Cytochrome P450 Isoforms and P-Glycoprotein

2008 ◽  
Vol 41 (05) ◽  
Author(s):  
E Jaquenoud-Sirot ◽  
B Knezevic ◽  
G Perla Morena ◽  
P Baumann ◽  
CB Eap
Keyword(s):  
Cytochrome P450 ◽  
P Glycoprotein ◽  
Cytochrome P450 Isoforms
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