Human Leukocyte Antigens (HLA) Class I and Class II on Sperm Cells Studied at the Serological, Cellular, and Genomic Levels

Infection with human papillomavirus (HPV), mainly HPV type 16, is the major etiologic factor associated with cervical cancer (CC), but HPV infection alone is not sufficient for progression of precursor lesions. Host genetic susceptibility may lead to abnormal immune response resulting from virus persistence. Several studies have suggested a possible association with specific human leukocyte antigen (HLA) class I and II alleles and CC, but results are not consistent. The association of genetic HLA class I (A and B) and HLA class II (DR*B1 and DQ*B1) haplotypes with HPV16-positive CC (n = 104) and base population controls (n = 104) was evaluated in this Mexican population study. Sequence-specific primer HLA genes were determined by polymerase chain reaction (PCR)-based methods in peripheral blood cell counts (PCR sequence-specific oligonucleotides). The cervical swabs of 208 women were tested for HPV16 by Hybrid Capture II. Allele and haplotype HLA frequencies, Hardy-Weinberg tests, and a haplotype homogeneity test were estimated using the Arlequin software v. 3.01. Odds ratio (OR) was calculated to compare cases and control women. Consistent associations across other studies in women with CC and infected by HPV16 were observed for HLA-DRB1*15 (OR, 3.9; 95% CI, 1.6-10.2) and the haplotype DRB1*15 DQB1*0602 (OR, 4.1; 95% CI, 1.4-12.7) compared with control women. The HLA-A2-B44-DR4-DQ*0302, HLA-A24-B35-DR16-DQ*0301, and HLA-A2-B40-DR4-DQ*0302 haplotypes showed a positive association with CC (OR, >1), whereas HLA-A2-B39-DR4-DQ*0302, HLA-A24-B35-DR4-DQ*0302, and HLA-A68-B40-DR4-DQ*0302 showed a negative association (OR, <1). These results support the hypothesis that some HLA class I and II haplotypes could be involved with susceptibility for developing CC.Abbreviations:Cervical Cancer-CC, confidence interval-CI, human leukocyte antigens-HLA, human papillomavirus-HPV, odds ratio-OR, polymerase chain reaction-PCR, relative risk-RR, relative light units-RLU, ribonucleic acid-RNA, sequence-sensitive oligonucleotide-SSO

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Genetic associations of killer immunoglobulin like receptors and class I human leukocyte antigens on childhood acute lymphoblastic leukemia among north Indians

Human Immunology ◽

10.1016/j.humimm.2015.10.009 ◽

2016 ◽

Vol 77 (1) ◽

pp. 41-46 ◽

Cited By ~ 8

Author(s):

Maneesh Kumar Misra ◽

Swayam Prakash ◽

Nirmalya Roy Moulik ◽

Archana Kumar ◽

Suraksha Agrawal

Keyword(s):

Acute Lymphoblastic Leukemia ◽

Lymphoblastic Leukemia ◽

Human Leukocyte ◽

Class I ◽

Childhood Acute Lymphoblastic Leukemia ◽

Human Leukocyte Antigens ◽

Genetic Associations ◽

Leukocyte Antigens ◽

North Indians

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Association of Human Leukocyte Antigens Class I and II Variants with Susceptibility to Pulmonary Tuberculosis in a Caucasian Population from Northern Spain

Mycobacterial Diseases ◽

10.4172/2161-1068.1000132 ◽

2013 ◽

Vol 03 (03) ◽

Author(s):

Gonzalo Ocejo-Vinyals J

Keyword(s):

Pulmonary Tuberculosis ◽

Human Leukocyte ◽

Caucasian Population ◽

Class I ◽

Human Leukocyte Antigens ◽

Northern Spain ◽

Leukocyte Antigens

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Stability of antigens on leukocytes in banked platelet concentrates: decline in HLA-DR antigen expression and mixed lymphocyte culture stimulating capacity following storage

Blood ◽

10.1182/blood.v72.3.867.867 ◽

1988 ◽

Vol 72 (3) ◽

pp. 867-872

Author(s):

ME Sherman ◽

WH Dzik

Keyword(s):

Hla Antigens ◽

Human Leukocyte ◽

Antigen Expression ◽

Class Ii ◽

Mixed Lymphocyte Culture ◽

Lymphocyte Culture ◽

Class I ◽

Platelet Concentrates ◽

Platelet Storage ◽

Leukocyte Antigens

Abstract Repeatedly transfused thrombocytopenic patients frequently form antibodies directed against human leukocyte antigens (HLA) and become unresponsive to random donor platelet transfusions. Although exposure to foreign antigens borne on donor leukocytes appears necessary to provoke primary sensitization, the stability of leukocyte antigens during routine platelet storage is largely unknown. Accordingly, we serially measured the expression of surface markers on leukocytes derived from platelet concentrates during storage using immunofluorescence and flow cytometry. Our results indicate that the expression of class I HLA antigens, Leu-4 (T cell), and HLe-1 (pan leukocyte) remained stable on lymphocytes under standard platelet storage conditions, but that the percentage of lymphocytes bearing class II HLA antigens declined significantly over time. This decline in lymphocyte HLA class II expression was associated with a significantly diminished ability of stored leukocytes to stimulate blastogenesis in mixed lymphocyte culture. However, leukocytes retained the ability to respond in mixed lymphocyte culture (MLC) following storage. We also performed studies on lymphocytes cultured in the presence of cyclohexamide, which suggested that the expression of class I HLA antigens and B2 microglobulin are highly sensitive to the inhibition of protein synthesis, whereas the expression of class II HLA antigens, Leu- 4, and HLe-1 are not. Our results may prove useful in understanding the mechanisms that lead to platelet refractoriness and in designing strategies to prevent HLA alloimmunization.

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