Autologous Transplantation of Adipose Tissue-Derived Stromal Cells Ameliorates Pulmonary Emphysema

2006 ◽  
Vol 6 (11) ◽  
pp. 2592-2600 ◽  
Author(s):  
N. Shigemura ◽  
M. Okumura ◽  
S. Mizuno ◽  
Y. Imanishi ◽  
T. Nakamura ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Stromal Cells ◽  
Pulmonary Emphysema ◽  
Autologous Transplantation

scholarly journals USE OF FATTY STROMAL CELLS IN THE COMPLEX TREATMENT OF TROPHIC ULCERS IN THE EXPERIMENT

2021 ◽  
pp. 14-22
Author(s):  
Volodymyr Lupaltsov ◽  
Andriy Yagniuk ◽  
Sergii Kitchenko ◽  
Irina Sorokina ◽  
Oksana Kaluzhina
Keyword(s):  
Adipose Tissue ◽  
Drug Therapy ◽  
Stromal Cells ◽  
Morphological Study ◽  
Venous Insufficiency ◽  
Autologous Transplantation ◽  
Experimental Studies ◽  
Control Group ◽  
Immunohistochemical Studies ◽  
Adipose Tissue Cells

Abstract. The aim of the study. The purpose of the research is to study the effectiveness of the treatment of trophic ulcers by autologous transplantation of stromal cells from adipose tissue in the experiment. Materials and methods of research. Experimental studies of the effectiveness of the treatment of trophic ulcers of venous etiology by autotransplantation of adipose tissue cells on 14 rabbits of the Chinchilla line, aged more than 4 months in both sexes, weighing 3 kilograms, are presented. After receiving the trophic ulcer, the rabbits were divided into 2 groups. A control group of 7 rabbits who used drug therapy and a core group of 7 rabbits who underwent autotransplantation of stromal cells from adipose tissue against the background of drug therapy. Both groups performed morphological and immunohistochemical studies. Results and conclusions. It is determined that the use of the method of autotransplantation of the stromo-vascular fraction of adipose tissue (SVF) in the treatment of trophic ulcer of venous etiology in the experiment in rabbits leads to acceleration of the processes in the healing of trophic ulcer, which is increased by the level of cytokyntinucleation, into more mature connective tissue. Keywords: mesenchymal stromal cells from adipose tissue, trophic ulcer, chronic venous insufficiency, experiment, morphological study, immunohistochemically research.

Cell therapy with adipose tissue-derived stem/stromal cells for elastase-induced pulmonary emphysema in rats

2012 ◽  
Vol 7 (4) ◽  
pp. 503-512 ◽  
Author(s):  
Naoki Furuya ◽  
Mitsuko Takenaga ◽  
Yuki Ohta ◽  
Yukie Tokura ◽  
Akemi Hamaguchi ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Cell Therapy ◽  
Stromal Cells ◽  
Pulmonary Emphysema

scholarly journals Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart

Genes ◽  
2019 ◽  
Vol 10 (6) ◽  
pp. 474 ◽  
Author(s):  
Polancec ◽  
Zenic ◽  
Hudetz ◽  
Boric ◽  
Jelec ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Stromal Cells ◽  
Autologous Transplantation ◽  
Stromal Vascular Fraction ◽  
Degenerative Joint Disease ◽  
Joint Disease ◽  
Dead Cell ◽  
Cell Detection ◽  
Loss Of Function ◽  
Cartilage Treatment

Osteoarthritis (OA) is a degenerative joint disease accompanied by pain and loss of function. Adipose tissue harbors mesenchymal stem/stromal cells (MSC), or medicinal signaling cells as suggested by Caplan (Caplan, 2017), used in autologous transplantation in many clinical settings. The aim of the study was to characterize a stromal vascular fraction from microfragmented lipoaspirate (SVF-MLA) applied for cartilage treatment in OA and compare it to that of autologous lipoaspirate (SVF-LA). Samples were first stained using a DuraClone SC prototype tube for the surface detection of CD31, CD34, CD45, CD73, CD90, CD105, CD146 and LIVE/DEAD Yellow Fixable Stain for dead cell detection, followed by DRAQ7 cell nuclear dye staining, and analyzed by flow cytometry. In SVF-LA and SVF-MLA samples, the following population phenotypes were identified within the CD45- fraction: CD31+CD34+CD73±CD90±CD105±CD146± endothelial progenitors (EP), CD31+CD34-CD73±CD90±CD105-CD146± mature endothelial cells, CD31-CD34-CD73±CD90+CD105-CD146+ pericytes, CD31-CD34+CD73±CD90+CD105-CD146+ transitional pericytes, and CD31-CD34+CD73highCD90+CD105-CD146- supra-adventitial-adipose stromal cells (SA-ASC). The immunophenotyping profile of SVF-MLA was dominated by a reduction of leukocytes and SA-ASC, and an increase in EP, evidencing a marked enrichment of this cell population in the course of adipose tissue microfragmentation. The role of EP in pericyte-primed MSC-mediated tissue healing, as well as the observed hormonal implication, is yet to be investigated.

scholarly journals Processing and Ex Vivo Expansion of Adipose Tissue-Derived Mesenchymal Stem/Stromal Cells for the Development of an Advanced Therapy Medicinal Product for Use in Humans

Cells ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1908
Author(s):  
Anna Labedz-Maslowska ◽  
Agnieszka Szkaradek ◽  
Tomasz Mierzwinski ◽  
Zbigniew Madeja ◽  
Ewa Zuba-Surma
Keyword(s):  
Adipose Tissue ◽  
Medicinal Product ◽  
Stromal Cells ◽  
Manufacturing Process ◽  
Cellular Therapy ◽  
Ex Vivo ◽  
Ex Vivo Expansion ◽  
Advanced Therapy Medicinal Product ◽  
Differentiation Potential ◽  
Advanced Therapy

Adipose tissue (AT) represents a commonly used source of mesenchymal stem/stromal cells (MSCs) whose proregenerative potential has been widely investigated in multiple clinical trials worldwide. However, the standardization of the manufacturing process of MSC-based cell therapy medicinal products in compliance with the requirements of the local authorities is obligatory and will allow us to obtain the necessary permits for product administration according to its intended use. Within the research phase (RD), we optimized the protocols used for the processing and ex vivo expansion of AT-derived MSCs (AT-MSCs) for the development of an Advanced Therapy Medicinal Product (ATMP) for use in humans. Critical process parameters (including, e.g., the concentration of enzyme used for AT digestion, cell culture conditions) were identified and examined to ensure the high quality of the final product containing AT-MSCs. We confirmed the identity of isolated AT-MSCs as MSCs and their trilineage differentiation potential according to the International Society for Cellular Therapy (ISCT) recommendations. Based on the conducted experiments, in-process quality control (QC) parameters and acceptance criteria were defined for the manufacturing of hospital exemption ATMP (HE-ATMP). Finally, we conducted a validation of the manufacturing process in a GMP facility. In the current study, we presented a process approach leading to the optimization of processing and the ex vivo expansion of AT-MSCs for the development of ATMP for use in humans.

Molecular mechanisms underlying human adipose tissue-derived stromal cells differentiation into a hepatocyte-like phenotype

2010 ◽  
Vol 42 (12) ◽  
pp. 895-901 ◽  
Author(s):  
Nathalie Saulnier ◽  
Anna Chiara Piscaglia ◽  
Maria Ausiliatrice Puglisi ◽  
Marta Barba ◽  
Vincenzo Arena ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Stromal Cells ◽  
Molecular Mechanisms ◽  
Human Adipose Tissue
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