scholarly journals USE OF FATTY STROMAL CELLS IN THE COMPLEX TREATMENT OF TROPHIC ULCERS IN THE EXPERIMENT

2021 ◽  
pp. 14-22
Author(s):  
Volodymyr Lupaltsov ◽  
Andriy Yagniuk ◽  
Sergii Kitchenko ◽  
Irina Sorokina ◽  
Oksana Kaluzhina
Keyword(s):  
Adipose Tissue ◽  
Drug Therapy ◽  
Stromal Cells ◽  
Morphological Study ◽  
Venous Insufficiency ◽  
Autologous Transplantation ◽  
Experimental Studies ◽  
Control Group ◽  
Immunohistochemical Studies ◽  
Adipose Tissue Cells

Abstract. The aim of the study. The purpose of the research is to study the effectiveness of the treatment of trophic ulcers by autologous transplantation of stromal cells from adipose tissue in the experiment. Materials and methods of research. Experimental studies of the effectiveness of the treatment of trophic ulcers of venous etiology by autotransplantation of adipose tissue cells on 14 rabbits of the Chinchilla line, aged more than 4 months in both sexes, weighing 3 kilograms, are presented. After receiving the trophic ulcer, the rabbits were divided into 2 groups. A control group of 7 rabbits who used drug therapy and a core group of 7 rabbits who underwent autotransplantation of stromal cells from adipose tissue against the background of drug therapy. Both groups performed morphological and immunohistochemical studies. Results and conclusions. It is determined that the use of the method of autotransplantation of the stromo-vascular fraction of adipose tissue (SVF) in the treatment of trophic ulcer of venous etiology in the experiment in rabbits leads to acceleration of the processes in the healing of trophic ulcer, which is increased by the level of cytokyntinucleation, into more mature connective tissue. Keywords: mesenchymal stromal cells from adipose tissue, trophic ulcer, chronic venous insufficiency, experiment, morphological study, immunohistochemically research.

THE USE OF BIOTECHNOLOGY IN THE PREVENTION OF FAILURE IN THE IMPOSITION OF SMALL INTESTINAL ANASTOMOSES IN THE EXPERIMENT

2020 ◽  
pp. 87-91
Author(s):  
M. Tymchenko ◽  
Yu. Ivanova ◽  
V. Padalko ◽  
H. Sheremet ◽  
O. Kozlova ◽  
...  
Keyword(s):  
Mucous Membrane ◽  
Intestinal Mucosa ◽  
Intestinal Anastomosis ◽  
Experimental Studies ◽  
Operating Conditions ◽  
Laboratory Animals ◽  
Control Group ◽  
Intestinal Anastomoses ◽  
Series Of Experiments ◽  
Immunohistochemical Studies

Summary. The aimof the study is to develop a method of sealing the sutured of the small intestine anastomosis in conditions of high risk anastomosis leakege by stopping the inflammation’ cascade in experiment. Materials and methods.The work was experimental in nature, was performed in the SI «ZIGUS NAMSU» in collaboration with the Scientific Research Institute of Biology V. N. Karazin KhNU in the period from 2018 to 2019. To increase the tightness of the intestinal anastomoses applied under conditions of laparatomy, experimental studies were conducted on laboratory animals. The studies were carried out after the permission of the ethics committee SI «ZIGUS NAMSU» in compliance with all ethical requirements for the humane treatment of animals.Surgical operations in two series of experiments were performed under operating conditions under general anesthesia on 10 white sexually mature linear rats – Wistar males, 12-18 months old, weighing about 250 grams. All morphological material (the first and second series of experiments) was marked and fixed in a 10% solution of neutral formalin. Subsequently, paraffin sections were prepared according to the generally accepted histotechnical method and stained with hematoxylin - eosin; according to Van Gieson and Mallory, an immunohistochemical study was used to study microenvironment factors. Results and Discussion. Immunohistochemical examination of the intestine was carried out in several stages, at the first stage we took intestinal tissue during the surgery to form the intestinal anastomosis, before the introduction of allogeneic cell transplants. Also carried out immunohistochemical studies of the emerging mucous membrane at various times after the formation of insolvency of intestinal anastomoses in both studied groups. When using a cell allograft, the mechanisms of repair of the intestinal mucosa include the appearance of cells that carry the expression of markers of mesenchymal and epithelial differentiation (Vim, CD34, CKW, Ecad), and are usually localized in the walls of blood vessels of granulation tissue, as well as in areas of the repairing epithelium. In addition, in the main group, only 7 days after the reaction to cytokeratins (CKW) and Ecad, areas of the repairing epithelium were detected, while the visualization of the epithelium in the control group did not begin even after 10 days. The analysis of immunophenotypes of repairing cells allows us to suggest the participation of mesenchymal-epithelial transformation mechanisms in this process. Conclusions.Thus, studies have shown that the use of cell transplantation of culture of allogeneic cells of the intestine leads to the reconstruction of the intestinal mucosa in a fairly short time. Conducted histological and immunohistochemical studies showed that in the vast majority of animals (77.2%) 10-14 days after the operation, an almost unchanged mucous membrane forms with the preservation of its elasticity.

scholarly journals Efficacy of Platelet-Rich Plasma Containing Xenogenic Adipose Tissue-Derived Stromal Cells on Restoring Intervertebral Disc Degeneration: A Preclinical Study in a Rabbit Model

2019 ◽  
Vol 2019 ◽  
pp. 1-7
Author(s):  
Chao Ma ◽  
Ran Wang ◽  
Dingliang Zhao ◽  
Naikun Wang ◽  
Ying Han ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Stromal Cells ◽  
Signal Intensity ◽  
Intervertebral Disc Degeneration ◽  
Platelet Rich Plasma ◽  
Rabbit Model ◽  
Control Group ◽  
Arterial Blood

Objective. Platelet-rich plasma (PRP) containing multiple growth factors is a promising strategy for disc degeneration. Thus, this study hypothesizes that the combination of PRP and adipose tissue-derived stromal cells (ADSCs) may repair degenerative disc more effectively than using each one of them alone. Methods. The model of early intervertebral disc degeneration was induced by annular puncture in the New Zealand rabbit. Autologous PRP was extracted from fresh arterial blood by using two centrifugation techniques. ADSC was offered by the Center for Clinic Stem Cell Research. Four weeks after the first experiment, PRP or ADSCs or a combination of PRP and ADSCs was injected into the punctured intervertebral disc. Four weeks later, disc height and signal intensity on T2-weighted magnetic resonance imaging (MRI) were assessed. Results. One month after puncture, we detected relatively narrow discs and lower signal intensity in MRI T2-weighted images. At four weeks after injection, the PRP-ADSC group statistically significantly restored discs, compared with PRP, ADSCs, or negative control group. Conclusions. The combination of PRP and ADSCs shows an effective potential to restore degenerated intervertebral discs in the rabbit.

Autologous Transplantation of Adipose Tissue-Derived Stromal Cells Ameliorates Pulmonary Emphysema

2006 ◽  
Vol 6 (11) ◽  
pp. 2592-2600 ◽  
Author(s):  
N. Shigemura ◽  
M. Okumura ◽  
S. Mizuno ◽  
Y. Imanishi ◽  
T. Nakamura ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Stromal Cells ◽  
Pulmonary Emphysema ◽  
Autologous Transplantation

Vitamin E and Selenium Facilitate the Osteogenesis and Adipogenesis of the Human Adipose Tissue-Derived Mesenchymal Stem/Stromal Cells

2020 ◽  
Author(s):  
Zeynab Javadi ◽  
Alireza Khoshdel ◽  
Mohammad Ali Fahmidehkar ◽  
Mohammad Reza Hajizadeh ◽  
Mehdi Mahmoodi ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Vitamin E ◽  
Lipid Droplet ◽  
Mtt Assay ◽  
Stromal Cells ◽  
Human Adipose Tissue ◽  
Control Group ◽  
Alizarin Red ◽  
Matrix Mineralization ◽  
Mesodermal Cell

Background and Aims: Previous studies have shown that adipose-derived mesenchymal stem/ stromal cells are one of the sources of mesenchymal stem cells (MSCs) with the capacity to differentiate into various mesodermal cell lineages. MSCs with cytokines secretion capability, which contributes to repair damaged tissues have gained wide credence for future cell-based therapeutic applications. In this study, the effect of the different dosages of vitamin E and Selenium was assessed on the stemness of the human adipose tissue-derived MSCs (AD-MSCs). Materials and Methods: Following 24 hours of cell treatments with different dosages of vitamin E and Selenium, MTT assay was used to assess the effect of them on cell proliferation. Moreover, the stemness of the AD-MSCs was assessed using osteogenic and adipogenic induction medium supplemented with the different dosages of the vitamin E and Selenium. Finally, Alizarin red and Oil-red O staining were performed to detect matrix mineralization and lipid droplet accumulation, respectively. Results: MTT data revealed that the optimal concentration for vitamin E and Selenium were 125 µM and 121 µM for the viability of the AD-MSCs. Moreover, the effect of vitamin E and Selenium were assessed by osteogenic and adipogenic differentiation by optimal dosages obtained by MTT assay, respectively. Maximum mineralization and lipid droplet aggregation of the differentiated cells were detected at IC50 in comparison with the control group. Conclusions: These results suggest that different dosages of vitamin E and Selenium could have various impacts on the proliferation and differentiation induction of human AD-MSCs.

scholarly journals Andrographolide Promotes Neural Differentiation of Rat Adipose Tissue-Derived Stromal Cells through Wnt/β-Catenin Signaling Pathway

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Yan Liang ◽  
Miao Li ◽  
Tao Lu ◽  
Wang Peng ◽  
Jian-Huang Wu
Keyword(s):  
Adipose Tissue ◽  
Stem Cell ◽  
Stromal Cells ◽  
Neural Differentiation ◽  
Molecular Mechanisms ◽  
Adult Stem Cell ◽  
High Yield ◽  
Control Group ◽  
Stem Cell Markers ◽  
Neural Lineage

Adipose tissue-derived stromal cells (ADSCs) are a high-yield source of pluripotent stem cells for use in cell-based therapies. We explored the effect of andrographolide (ANDRO, one of the ingredients of the medicinal herb extract) on the neural differentiation of rat ADSCs and associated molecular mechanisms. We observed that rat ADSCs were small and spindle-shaped and expressed multiple stem cell markers including nestin. They were multipotent as evidenced by adipogenic, osteogenic, chondrogenic, and neural differentiation under appropriate conditions. The proportion of cells exhibiting neural-like morphology was higher, and neurites developed faster in the ANDRO group than in the control group in the same neural differentiation medium. Expression levels of the neural lineage markers MAP2, tau, GFAP, and β-tubulin III were higher in the ANDRO group. ANDRO induced a concentration-dependent increase in Wnt/β-catenin signaling as evidenced by the enhanced expression of nuclear β-catenin and the inhibited form of GSK-3β (pSer9). Thus, this study shows for the first time how by enhancing the neural differentiation of ADSCs we expect that ANDRO pretreatment may increase the efficacy of adult stem cell transplantation in nervous system diseases, but more exploration is needed.

scholarly journals Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart

Genes ◽  
2019 ◽  
Vol 10 (6) ◽  
pp. 474 ◽  
Author(s):  
Polancec ◽  
Zenic ◽  
Hudetz ◽  
Boric ◽  
Jelec ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Stromal Cells ◽  
Autologous Transplantation ◽  
Stromal Vascular Fraction ◽  
Degenerative Joint Disease ◽  
Joint Disease ◽  
Dead Cell ◽  
Cell Detection ◽  
Loss Of Function ◽  
Cartilage Treatment

Osteoarthritis (OA) is a degenerative joint disease accompanied by pain and loss of function. Adipose tissue harbors mesenchymal stem/stromal cells (MSC), or medicinal signaling cells as suggested by Caplan (Caplan, 2017), used in autologous transplantation in many clinical settings. The aim of the study was to characterize a stromal vascular fraction from microfragmented lipoaspirate (SVF-MLA) applied for cartilage treatment in OA and compare it to that of autologous lipoaspirate (SVF-LA). Samples were first stained using a DuraClone SC prototype tube for the surface detection of CD31, CD34, CD45, CD73, CD90, CD105, CD146 and LIVE/DEAD Yellow Fixable Stain for dead cell detection, followed by DRAQ7 cell nuclear dye staining, and analyzed by flow cytometry. In SVF-LA and SVF-MLA samples, the following population phenotypes were identified within the CD45- fraction: CD31+CD34+CD73±CD90±CD105±CD146± endothelial progenitors (EP), CD31+CD34-CD73±CD90±CD105-CD146± mature endothelial cells, CD31-CD34-CD73±CD90+CD105-CD146+ pericytes, CD31-CD34+CD73±CD90+CD105-CD146+ transitional pericytes, and CD31-CD34+CD73highCD90+CD105-CD146- supra-adventitial-adipose stromal cells (SA-ASC). The immunophenotyping profile of SVF-MLA was dominated by a reduction of leukocytes and SA-ASC, and an increase in EP, evidencing a marked enrichment of this cell population in the course of adipose tissue microfragmentation. The role of EP in pericyte-primed MSC-mediated tissue healing, as well as the observed hormonal implication, is yet to be investigated.

Clinical efficacy of osteopathic correction in the complex treatment of patients with unoperated open-angle glaucoma

2020 ◽  
pp. 97-105
Author(s):  
E. N. Simakova ◽  
O. V. Stenkova
Keyword(s):  
Intraocular Pressure ◽  
Drug Therapy ◽  
Primary Open Angle Glaucoma ◽  
Open Angle Glaucoma ◽  
Randomized Study ◽  
Main Group ◽  
Control Group ◽  
Open Angle ◽  
Complex Treatment ◽  
Hypotensive Drug

Introduction. Glaucoma is one of the most significant eye diseases. It is often diagnosed, not always amenable to therapy, and can lead to a complete loss of visual functions. In recent years, the method of osteopathic correction has become widespread as one of the effective methods of treatment and rehabilitation of patients with pathologies of various body systems. In the pathogenesis of glaucoma, it is customary to distinguish a dystrophic concept, which considers primary open-angle glaucoma as a result of dystrophic changes in the connective tissue, as well as in the endothelial lining of the trabeculae and Schlemm′s canal, especially destructive changes in mitochondria and the alteration of their functional activity. A vascular concept is also distinguished. According to this concept, the central link in the pathogenesis of glaucoma is circulatory disorder in the ciliary vessels, ocular artery, and major vessels of the head and neck, it can be assumed that osteopathic correction in the treatment of patients with open-angle glaucoma will be pathogenetically substantiated and will have a positive effect on intraocular pressure and trophicity of the optic nerve. The goal of research — to study the influence of in osteopathic correction on the nature of unoperated glaucoma (stage IIA) and to substantiate the possibility of using osteopathic correction in the complex treatment of patients with this pathology.Materials and methods. A prospective controlled randomized study was conducted at 52 city polyclinics, branch 3, Moscow, from January 2018 to January 2019. 40 patients (70 eyes) aged 50 to 75 years with primary open-angle glaucoma IIA stage were examined. At this stage of the disease, patients most often seek medical care and the issue of conservative management is primarily considered. All patients were divided into two groups of 20 people: the main group and the control group. The treatment in the main group included hypotensive drug therapy and osteopathic correction. Patients of the control group received only drug therapy. All patients underwent ophthalmic (visometry, tonometry, perimetry) and osteopathic examination twice: before the treatment and after 3 months.Results. For patients with primary open-angle IIA non-operated glaucoma, regional (most often regions of the head, neck, dura mater) and local (abdominal diaphragm, iliac bones, hip and knee joints) somatic dysfunctions were the most typical. In the main group a statistically significant decrease in the frequency and severity of dysfunctions at all levels was stated. Also, in patients receiving osteopathic correction, a significant decrease in the level of intraocular pressure and perimetric indices was noted. In patients of the control group, no reliable changes in these indicators were obtained.Conclusion. The results obtained indicate that osteopathic correction is clinically effective in the complex treatment of patients with primary open-angle II A glaucoma.

A new method for the experimental induction of secundary biliary cirrhosis in wistar rats

2001 ◽  
Vol 16 (2) ◽  
pp. 75-81 ◽  
Author(s):  
Gracinda De Lourdes Jorge ◽  
Luiz Sergio Leonardi ◽  
Ilka de Fatima Santana Ferreira Boin ◽  
Orlando de Castro e Silva Jr ◽  
Cecilia Amelia Fazzio Escanhoela
Keyword(s):  
Bile Duct ◽  
Morphological Study ◽  
Wistar Rats ◽  
Hepatic Duct ◽  
Control Group ◽  
Sham Operation ◽  
Biliary Cirrhosis ◽  
Technical Failure ◽  
Duct Obstruction ◽  
Duct Ligation

The aim of this study was to describe a method for the induction of experimental secondary biliary fibrosis (SBF). Forty-seven Wistar rats were submitted to hepatic duct obstruction (OB group) for thirty days without ligature, section or cannulization causing interruption of biliary flow. This technique was carried out by simple traction of the bile duct passing it through the xiphoid appendix. Nine rats were submitted to a sham operation for bile duct stricture and seven rats comprised the control group. Blood samples were collected for the measurement of total bilirubin (TB), alkaline phosphatase (AP), alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Liver fragments were removed for morphological study. Thirty days after surgery TB, AP, ALT and AST levels were significantly increased in the hepatic duct ligation group compared to the sham operated group and the presence of SBF in the OB group was confirmed by morphological study of the liver. There was technical failure in 31.92% cases. The survival was 100% at fifteen days and 82.97% at the end of the experiment. We concluded that this simple surgical technique may be used to study the consequence of bile duct obstruction which could be a reversible process depending on the obstruction time. This technique can be carried out from cholestasis to fibrosis.

The influence of probiotic drug Biolax-U on morphological and biochemical parameters of blood in cows

2020 ◽  
pp. 70-76
Author(s):  
A. Farkhutdinova
Keyword(s):  
Biochemical Parameters ◽  
Hematological Parameters ◽  
Experimental Studies ◽  
Positive Influence ◽  
Biologically Active ◽  
Feed Consumption ◽  
Control Group ◽  
Animal Blood ◽  
Black And White ◽  
White Breed

The expediency of using the probiotic drug Biolax-U in the rations of lactating and dry cows of Black-and-White breed has been studied and scientifically proved. The influence of this drug on the morphological and biochemical parameters of animal blood has been determined. The composition of the probiotic drug Biolax-U consists of eight strains of a balanced complex of living lactic acid bacteria, yeast and actinomycetes, as well as biologically active substances. For experimental studies conducted on 2 groups of cows have been selected by the method of pairs-analogs, the influence of feeding of feed have been prepared with the use of the microbiological drug with probiotic action Biolax-U on feed consumption, morphological and biochemical changes in blood has been studied. Analysis of the rations of cows indicates that the content of calcium, phosphorus, magnesium, sulfur and trace elements such as manganese, copper, zinc and cobalt in the feed has been reduced. It has been found by studies that the use of probiotic drug Biolax-U had the positive influence on the feed intake, digestibility and better assimilation of feed nutrients and contributed to the improvement of blood morphological and biochemical parameters. For example, in animals of the experimental group hemoglobin has increased by 3,6 % (Р < 0,05), red blood cells by 10,1 (P ≤ 0,05), the content of leukocytes by 4,43, carotene by 9,6, glucose by 3,4; reserve alkalinity by 15,6, calcium by 19,4 (P ≤ 0,01), and phosphorus by 18,4 % compared with the control group of animals. During the lactation significantly improved hematological parameters including alkalinity reserve by 5,11 %, calcium level by 11,1, phosphorus by 8,6, hemoglobin by 14,2 %, the content of erythrocytes by 12,3, leukocyte by 3,86, and carotene 11,1 % against the parameters of animals of control group. The improvement of morphological and biochemical parameters of blood of cows indicates about stimulating effect on hematopoiesis and the general clinical condition of animals that contributes to the improvement of metabolic processes when using feed prepared with the use of microbiological drug with probiotic action Biolax-U.

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