scholarly journals Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart

Genes ◽  
2019 ◽  
Vol 10 (6) ◽  
pp. 474 ◽  
Author(s):  
Polancec ◽  
Zenic ◽  
Hudetz ◽  
Boric ◽  
Jelec ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Stromal Cells ◽  
Autologous Transplantation ◽  
Stromal Vascular Fraction ◽  
Degenerative Joint Disease ◽  
Joint Disease ◽  
Dead Cell ◽  
Cell Detection ◽  
Loss Of Function ◽  
Cartilage Treatment

Osteoarthritis (OA) is a degenerative joint disease accompanied by pain and loss of function. Adipose tissue harbors mesenchymal stem/stromal cells (MSC), or medicinal signaling cells as suggested by Caplan (Caplan, 2017), used in autologous transplantation in many clinical settings. The aim of the study was to characterize a stromal vascular fraction from microfragmented lipoaspirate (SVF-MLA) applied for cartilage treatment in OA and compare it to that of autologous lipoaspirate (SVF-LA). Samples were first stained using a DuraClone SC prototype tube for the surface detection of CD31, CD34, CD45, CD73, CD90, CD105, CD146 and LIVE/DEAD Yellow Fixable Stain for dead cell detection, followed by DRAQ7 cell nuclear dye staining, and analyzed by flow cytometry. In SVF-LA and SVF-MLA samples, the following population phenotypes were identified within the CD45- fraction: CD31+CD34+CD73±CD90±CD105±CD146± endothelial progenitors (EP), CD31+CD34-CD73±CD90±CD105-CD146± mature endothelial cells, CD31-CD34-CD73±CD90+CD105-CD146+ pericytes, CD31-CD34+CD73±CD90+CD105-CD146+ transitional pericytes, and CD31-CD34+CD73highCD90+CD105-CD146- supra-adventitial-adipose stromal cells (SA-ASC). The immunophenotyping profile of SVF-MLA was dominated by a reduction of leukocytes and SA-ASC, and an increase in EP, evidencing a marked enrichment of this cell population in the course of adipose tissue microfragmentation. The role of EP in pericyte-primed MSC-mediated tissue healing, as well as the observed hormonal implication, is yet to be investigated.

Adipose-Derived Stem/Stromal Cells, Stromal Vascular Fraction, and Microfragmented Adipose Tissue

2022 ◽  
pp. 47-61
Author(s):  
Enrico Ragni ◽  
Marco Viganò ◽  
Paola De Luca ◽  
Edoardo Pedrini ◽  
Laura de Girolamo
Keyword(s):  
Adipose Tissue ◽  
Stromal Cells ◽  
Stromal Vascular Fraction

scholarly journals Evaluation of Three Devices for the Isolation of the Stromal Vascular Fraction from Adipose Tissue and for ASC Culture: A Comparative Study

2017 ◽  
Vol 2017 ◽  
pp. 1-14 ◽  
Author(s):  
Jonathan Rodriguez ◽  
Anne-Sophie Pratta ◽  
Nacira Abbassi ◽  
Hugo Fabre ◽  
Fanny Rodriguez ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Adipose Tissue ◽  
Medical Devices ◽  
Stromal Cells ◽  
Genetic Stability ◽  
Stromal Vascular Fraction ◽  
Reference Method ◽  
Proliferative Potential ◽  
Population Doublings ◽  
Multilineage Potential

Adipose-derived stem/stromal cells (ASCs) reside in the stromal vascular fraction (SVF) of adipose tissue (AT) and can be easily isolated. However, extraction of the SVF from lipoaspirate is a critical step in generating ASC, and semiautomated devices have been developed to enhance the efficacy and reproducibility of the outcomes and to decrease manipulation and contamination. In this study, we compared the reference method used in our lab for SVF isolation from lipoaspirate, with three medical devices: GID SVF-1™, Puregraft™, and Stem.pras®. Cell yield and their viability were evaluated as well as their phenotype with flow cytometry. Further on, we determined their proliferative potential using population doublings (PD), PD time (PDT), and clonogenicity assay (CFU-F). Finally, we checked their genetic stability using RT-qPCR for TERT mRNA assay and karyotyping as well as their multilineage potential including adipogenic, chondrogenic, and osteogenic differentiation. Our results demonstrate that all the devices allow the production of SVF cells with consistent yield and viability, in less time than the reference method. Expanded cells from the four methods showed no significant differences in terms of phenotype, proliferation capabilities, differentiation abilities, and genetic stability.

The Development of Facial Lipofilling from a Historical Point of View

2019 ◽  
Vol 35 (04) ◽  
pp. 358-367 ◽  
Author(s):  
Joris A. van Dongen ◽  
Joeri van Boxtel ◽  
Martin C. Harmsen ◽  
Hieronymus P. Stevens
Keyword(s):  
Adipose Tissue ◽  
Stromal Cells ◽  
Stromal Vascular Fraction ◽  
Point Of View ◽  
Congenital Defects ◽  
Skin Rejuvenation ◽  
Adipose Derived Stromal Cells ◽  
Enzymatic Isolation ◽  
The Face ◽  
Mechanical Isolation

AbstractLipofilling, the transplantation of adipose tissue, has already been used since the end of the 19th century. For decades, lipofilling was used to restore loss of volume due to aging, trauma, or congenital defects. Later on, the indications for the use of lipofilling expanded by treating aged skin, scars, and improving wound healing. The expansion was caused by the discovery of adipose derived stromal cells (ASCs) in adipose tissue and the development of very fine harvesting and injection cannulas which made it possible to inject small adipose tissue particles in small volume areas, such as the face. ASCs are multipotent stromal cells which reside in the stromal vascular fraction (SVF) of adipose tissue and are able to differentiate in multiple cell lineages and secrete a plurality of growth factors with regenerative potentials. The discovery of ASCs led toward more experimental cell-based therapies, that is, ASCs or SVF isolated by means of enzymatic isolation procedures. Later on, enzymatic isolation procedures were forbidden in many countries by legislation and were replaced by mechanical isolation procedures, such as the Nanofat and Fractionation of Adipose Tissue (FAT) procedures. The Nanofat procedure has been extensively investigated, especially as treatment for skin rejuvenation in the face. Though, substantial evidence is lacking for using facial lipofilling or any therapeutic component, that is, ASCs or SVF for skin rejuvenation to date. In contrast, facial lipofilling to restore loss of volume seems to be promising.

scholarly journals Macrodystrophia Lipomatosa: Radiologic-Pathologic Correlation

2011 ◽  
Vol 1 ◽  
pp. 18 ◽  
Author(s):  
Deepika Upadhyay ◽  
Umesh C Parashari ◽  
Sachin Khanduri ◽  
Samarjit Bhadury
Keyword(s):  
Adipose Tissue ◽  
Clinical Work ◽  
Degenerative Joint Disease ◽  
Tissue Growth ◽  
Lower Limbs ◽  
Joint Disease ◽  
Neurovascular Compression ◽  
Provisional Diagnosis ◽  
Macrodystrophia Lipomatosa ◽  
Work Up

Macrodystrophia lipomatosa (MDL) is a rare cause of congenital macrodactyly, characterised by progressive proliferation of all mesenchymal elements, with disproportionate increase in fibro-adipose tissue. It occurs most frequently in lower limbs along the distribution of the medial plantar nerve. MDL presents as localised gigantism of the hand or foot and comes to clinical attention for cosmetic reasons, mechanical problems secondary to degenerative joint disease, or development of neurovascular compression. Here, we report a case of MDL, with altered soft tissue growth due to an earlier surgery, making clinical diagnosis difficult. However, with a complete radio-clinical work-up and review of the history, a provisional diagnosis of MDL was made, which was confirmed by histopathology and during surgery.

Autologous Transplantation of Adipose Tissue-Derived Stromal Cells Ameliorates Pulmonary Emphysema

2006 ◽  
Vol 6 (11) ◽  
pp. 2592-2600 ◽  
Author(s):  
N. Shigemura ◽  
M. Okumura ◽  
S. Mizuno ◽  
Y. Imanishi ◽  
T. Nakamura ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Stromal Cells ◽  
Pulmonary Emphysema ◽  
Autologous Transplantation

scholarly journals FEATURES OF REPARATIVE REGENERATION OF ACHILLES TENDON IN THE CONDITIONS OF AUTOLOGOUS TRANSPLANTATION OF CELL POOL

2020 ◽  
Vol 5 (3) ◽  
pp. 46-50
Author(s):  
Natal'ya Slesarenko ◽  
Aleksei Zharikov
Keyword(s):  
Adipose Tissue ◽  
Achilles Tendon ◽  
Methodological Approach ◽  
Autologous Transplantation ◽  
Stromal Vascular Fraction ◽  
High Plasticity ◽  
Cellular Element ◽  
Blood Capillaries ◽  
Morphological Studies ◽  
Cell Pool

The aim of the study is identification of features of Achilles tendon healing in the conditions of auto-transplantation of the cellular element, reflecting the regenerative activity of stromal-vascular fraction. Modern regenerative treat-ment methods for animal with tendon injuries involve stromal-vascular cells application of adipose tissue. They represent cell pool with high plasticity, proliferative activity, and ability to secrete multiple angiogenic factors. How-ever, there is no data on morphological and morphometric regenerative equivalents of their use in case of tendon ligaments injury. Micro morphometrical indicators are presented that substantiate the effectiveness of using autolo-gous cellular elements in case of Achilles tendon injury. The influence of stromal-vascular fraction from rat adipose tissue on regenerative process during healing of an induced tendon tissue defect was evaluated which lead to an increase in the thickness of bundles of the first and the second orders in the experimental group, significant differ-ences in comparison with the control of the fibroblastic cell pool, and the number of blood capillaries of these ani-mals. Based on the positive data of the autologous cell pool on the regenerative ability of the Achilles tendon, its practicability for veterinary medicine was scientifically justified. The experimental model of the study is a fancy rat (n=20). Achilles tendon was the research material. A complex methodological approach was used, including ex-perimental modeling, micro morphological studies, micro morphometry, and statistical data processing. It was re-vealed that experimental animals injected with stromal-vascular fraction, micro morphometric transformations of connective tissue were detected, aimed at thickening of bundles of the first and second orders, the transition of fi-broblasts to structured and ordered fibrocytes (tenocytes), acceleration of collagenogenesis and vascularization enhancement.

scholarly journals The Role of Wnt Pathway in the Pathogenesis of OA and Its Potential Therapeutic Implications in the Field of Regenerative Medicine

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Maria De Santis ◽  
Berardo Di Matteo ◽  
Emanuele Chisari ◽  
Gilberto Cincinelli ◽  
Peter Angele ◽  
...  
Keyword(s):  
Total Joint Replacement ◽  
Platelet Rich Plasma ◽  
Weight Bearing ◽  
Degenerative Joint Disease ◽  
Joint Disease ◽  
Loss Of Function ◽  
Biological Drugs ◽  
Therapeutic Implications ◽  
Pubmed Database

Introduction. Osteoarthritis (OA) is a degenerative joint disease characterized by articular cartilage degradation, subchondral damage, and bone remodelling, affecting most commonly weight-bearing joints, such as the knee and hip. The loss of cartilage leads to joint space narrowing, pain, and loss of function which could ultimately require total joint replacement. The Wnt/β catenin pathway is involved in the pathophysiology of OA and has been proposed as a therapeutic target. Endogenous and pharmacological inhibitors of this pathway were recently investigated within innovative therapies including the use of platelet-rich plasma (PRP) and mesenchymal stem cells (MSCs). Methods. A review of the literature was performed on the PubMed database based on the following inclusion criteria: article written in English language in the last 20 years and dealing with (1) the role of Wnt-β catenin pathway in the pathogenesis of osteoarthritis and (2) pharmacologic or biologic strategies modulating the Wnt-β catenin pathway in the OA setting. Results. Evidences support that Wnt signalling pathway is likely linked to OA progression and severity. Its inhibition through natural antagonists and new synthetic or biological drugs shares the potential to improve the clinical condition of the patients by affecting the pathological activity of Wnt/β-catenin signalling. Conclusions. While further research is needed to better understand the mechanisms regulating the molecular interaction between OA regenerative therapies and Wnt, it seems that biologic therapies for OA exert modulation on Wnt/β catenin pathway that might be relevant in achieving the beneficial clinical effect of those therapeutic strategies.

Human mesenchymal adipose stromal cells from mature adipocyte fraction

2010 ◽  
Vol 5 (1) ◽  
pp. 47-58 ◽  
Author(s):  
Aušra Unguryte ◽  
Eiva Bernotiene ◽  
Algirdas Venalis
Keyword(s):  
Adipose Tissue ◽  
Stromal Cells ◽  
Stromal Vascular Fraction ◽  
Culture Method ◽  
Control Culture ◽  
Mature Adipocyte ◽  
Ceiling Culture ◽  
Bottom Cell ◽  
Cell Fractions ◽  
Proliferation Potential

AbstractMore effective techniques should be employed for isolation of human mesenchymal stromal cells derived from adipose tissue (ADSC), seeking to make adipose tissue biopsies smaller in volume and thus less invasive. In this study, we compared properties of ADSC isolated by several different methods from the same samples of adipose tissue in order to enhance yields of potential ADSC. The mature adipocyte fraction was investigated using the ceiling culture method, including both ceiling and bottom cell fractions, and the control culture method with standard amount of medium. The results were also compared using the stromal vascular fraction from the same samples. The most efficient was the bottom cell population isolated from the mature adipocyte fraction by ceiling culture method. These cells readily differentiated into osteogenic, adipogenic and chondrogenic lineages and, similar to stromal vascular fraction cells, displayed high proliferation potential. Cultures of mature adipocyte fractions with standard amount of medium were considerably less effective. Mature adipocyte fractions yields large quantities of adipose-derived stem cells that have properties comparable with stromal vascular fraction cells suitable for tissue regeneration, especially when only small biopsies can be taken.

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