Localization of Seed Oil Body Proteins in Tobacco Protoplasts Reveals Specific Mechanisms of Protein Targeting to Leaf Lipid Droplets

2011 ◽  
Vol 53 (11) ◽  
pp. 858-868 ◽  
Author(s):  
Stefania De Domenico ◽  
Stefania Bonsegna ◽  
Marcello Salvatore Lenucci ◽  
Palmiro Poltronieri ◽  
Gian Pietro Di Sansebastiano ◽  
...  
Keyword(s):  
Lipid Droplets ◽  
Seed Oil ◽  
Protein Targeting ◽  
Oil Body ◽  
Tobacco Protoplasts

scholarly journals OPA1-anchored PKA phosphorylates perilipin 1 on S522 and S497 in adipocytes differentiated from human adipose stem cells

2018 ◽  
Vol 29 (12) ◽  
pp. 1487-1501 ◽  
Author(s):  
Marie Rogne ◽  
Dinh-Toi Chu ◽  
Thomas M. Küntziger ◽  
Maria-Niki Mylonakou ◽  
Philippe Collas ◽  
...  
Keyword(s):  
Stem Cells ◽  
Lipid Droplets ◽  
Protein Targeting ◽  
Adipose Stem Cells ◽  
Adrenergic Control ◽  
Anchoring Protein ◽  
Adipocytic Differentiation ◽  
First Time ◽  
Human Adipose Stem Cells ◽  
Perilipin 1

Optic atrophy 1 (OPA1) is the A-kinase anchoring protein targeting the pool of protein kinase A (PKA) responsible for perilipin 1 phosphorylation, a gatekeeper for lipolysis. However, the involvement of OPA1-bound PKA in the downstream regulation of lipolysis is unknown. Here we show up-regulation and relocation of OPA1 from mitochondria to lipid droplets during adipocytic differentiation of human adipose stem cells. We employed various biochemical and immunological approaches to demonstrate that OPA1-bound PKA phosphorylates perilipin 1 at S522 and S497 on lipolytic stimulation. We show that the first 30 amino acids of OPA1 are essential for its lipid droplet localization as is OMA1-dependent processing. Finally, our results indicate that presence of OPA1 is necessary for lipolytic phosphorylation of downstream targets. Our results show for the first time, to our knowledge, how OPA1 mediates adrenergic control of lipolysis in human adipocytes by regulating phosphorylation of perilipin 1.

scholarly journals Arf1/COPI machinery acts directly on lipid droplets and enables their connection to the ER for protein targeting

eLife ◽  
2014 ◽  
Vol 3 ◽  
Author(s):  
Florian Wilfling ◽  
Abdou Rachid Thiam ◽  
Maria-Jesus Olarte ◽  
Jing Wang ◽  
Rainer Beck ◽  
...  
Keyword(s):  
Surface Tension ◽  
Lipid Droplets ◽  
Protein Targeting ◽  
Neutral Lipids ◽  
Vesicle Trafficking ◽  
Metabolic Energy ◽  
Key Enzymes ◽  
Specific Proteins ◽  
Oil Water

Lipid droplets (LDs) are ubiquitous organelles that store neutral lipids, such as triacylglycerol (TG), as reservoirs of metabolic energy and membrane precursors. The Arf1/COPI protein machinery, known for its role in vesicle trafficking, regulates LD morphology, targeting of specific proteins to LDs and lipolysis through unclear mechanisms. Recent evidence shows that Arf1/COPI can bud nano-LDs (∼60 nm diameter) from phospholipid-covered oil/water interfaces in vitro. We show that Arf1/COPI proteins localize to cellular LDs, are sufficient to bud nano-LDs from cellular LDs, and are required for targeting specific TG-synthesis enzymes to LD surfaces. Cells lacking Arf1/COPI function have increased amounts of phospholipids on LDs, resulting in decreased LD surface tension and impairment to form bridges to the ER. Our findings uncover a function for Arf1/COPI proteins at LDs and suggest a model in which Arf1/COPI machinery acts to control ER-LD connections for localization of key enzymes of TG storage and catabolism.

scholarly journals Characteristics of Paeonia ostii seed oil body and OLE17.5 determining oil body morphology

2020 ◽  
Vol 319 ◽  
pp. 126548
Author(s):  
Daqiu Zhao ◽  
Tingting Li ◽  
Zhiyuan Li ◽  
Jing Sun ◽  
Jun Tao
Keyword(s):  
Seed Oil ◽  
Oil Body ◽  
Body Morphology

scholarly journals Seed Oil Body Ontogeny

2009 ◽  
Vol 15 (S2) ◽  
pp. 874-875 ◽  
Author(s):  
E Herman
Keyword(s):  
Seed Oil ◽  
Oil Body

Extended abstract of a paper presented at Microscopy and Microanalysis 2009 in Richmond, Virginia, USA, July 26 – July 30, 2009

scholarly journals New Insights Into the Role of Seed Oil Body Proteins in Metabolism and Plant Development

2019 ◽  
Vol 10 ◽  
Author(s):  
Qun Shao ◽  
Xiaofan Liu ◽  
Tong Su ◽  
Changle Ma ◽  
Pingping Wang
Keyword(s):  
Plant Development ◽  
Seed Oil ◽  
Oil Body

scholarly journals Mechanisms of protein targeting to lipid droplets: A unified cell biological and biophysical perspective

2020 ◽  
Vol 108 ◽  
pp. 4-13 ◽  
Author(s):  
Ravi Dhiman ◽  
Stefanie Caesar ◽  
Abdou Rachid Thiam ◽  
Bianca Schrul
Keyword(s):  
Lipid Droplets ◽  
Protein Targeting

scholarly journals Mechanism of Protein Targeting to Lipid Droplets

2018 ◽  
Vol 114 (3) ◽  
pp. 70a
Author(s):  
Morris E. Sharp ◽  
Coline Prevost ◽  
Maria-Jesus Olarte ◽  
Robert V. Farese ◽  
Tobias C. Walther ◽  
...  
Keyword(s):  
Lipid Droplets ◽  
Protein Targeting

scholarly journals Author response: Arf1/COPI machinery acts directly on lipid droplets and enables their connection to the ER for protein targeting

2013 ◽  
Author(s):  
Florian Wilfling ◽  
Abdou Rachid Thiam ◽  
Maria-Jesus Olarte ◽  
Jing Wang ◽  
Rainer Beck ◽  
...  
Keyword(s):  
Lipid Droplets ◽  
Protein Targeting ◽  
Author Response

Sunflower-seed oil body emulsions: Rheology and stability assessment of a natural emulsion

2008 ◽  
Vol 22 (7) ◽  
pp. 1224-1232 ◽  
Author(s):  
D.A. White ◽  
I.D. Fisk ◽  
J.R. Mitchell ◽  
B. Wolf ◽  
S.E. Hill ◽  
...  
Keyword(s):  
Sunflower Seed ◽  
Seed Oil ◽  
Oil Body ◽  
Stability Assessment ◽  
Sunflower Seed Oil

The biogenesis of the plant seed oil body: Oleosin protein is synthesised by ER-bound ribosomes

1999 ◽  
Vol 37 (6) ◽  
pp. 481-490 ◽  
Author(s):  
Frédéric Beaudoin ◽  
Dominic J. Lacey ◽  
Johnathan A. Napier
Keyword(s):  
Seed Oil ◽  
Oil Body ◽  
Plant Seed ◽  
Plant Seed Oil
Sign in / Sign up

Export Citation Format

Share Document