Effects of goat manure and inorganic phosphate addition on soil inorganic and microbial biomass phosphorus fractions under laboratory incubation conditions

Slices of cat brain respiring in a Krebs–Ringer bicarbonate medium were found to incorporate inorganic phosphate labelled with P32 into two non-nucleotide protein-bound phosphorus fractions, which are referred to as the residue organic phosphorus (ROP) and the "phosphoprotein" (PP), respectively. The addition of glucose or mannose to the medium increased the incorporation into both fractions. The addition of fructose, galactose, pyruvate, lactate, succinate, or L-glutamate failed to increase the incorporation into either fraction.Anaerobic conditions, homogenization of the tissue, or the addition of a wide range of metabolic inhibitors (cyanide, azide, iodoacetate, fluoride, nembutal, malononitrile, chloretone) inhibited the incorporation. The incorporation was also inhibited by 2,4-dinitrophenol in concentrations that do not inhibit oxygen consumption.These results are compared and contrasted with previous findings on the incorporation of P32 into the lipid phosphorus and pentosenucleic acid of brain slices.

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Phosphorus fractions, microbial biomass and enzyme activities in some alkaline calcareous subtropical soils

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb11.3483 ◽

2012 ◽

Vol 11 (21) ◽

Author(s):

Muhammad Asghar Malik

Keyword(s):

Microbial Biomass ◽

Enzyme Activities ◽

Phosphorus Fractions ◽

Subtropical Soils

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INCORPORATION OF RADIOACTIVE PHOSPHATE INTO NON-NUCLEOTIDE PROTEIN-BOUND PHOSPHORUS FRACTIONS OF RESPIRING CAT BRAIN SLICES

Canadian Journal of Biochemistry and Physiology ◽

10.1139/o54-055 ◽

1954 ◽

Vol 32 (5) ◽

pp. 504-514 ◽

Cited By ~ 19

Author(s):

Muriel Findlay ◽

K. P. Strickland ◽

R. J. Rossiter

Keyword(s):

Inorganic Phosphate ◽

Organic Phosphorus ◽

Brain Slices ◽

Phosphorus Fractions ◽

Metabolic Inhibitors ◽

Lipid Phosphorus ◽

Wide Range ◽

Cat Brain

Slices of cat brain respiring in a Krebs–Ringer bicarbonate medium were found to incorporate inorganic phosphate labelled with P32 into two non-nucleotide protein-bound phosphorus fractions, which are referred to as the residue organic phosphorus (ROP) and the "phosphoprotein" (PP), respectively. The addition of glucose or mannose to the medium increased the incorporation into both fractions. The addition of fructose, galactose, pyruvate, lactate, succinate, or L-glutamate failed to increase the incorporation into either fraction.Anaerobic conditions, homogenization of the tissue, or the addition of a wide range of metabolic inhibitors (cyanide, azide, iodoacetate, fluoride, nembutal, malononitrile, chloretone) inhibited the incorporation. The incorporation was also inhibited by 2,4-dinitrophenol in concentrations that do not inhibit oxygen consumption.These results are compared and contrasted with previous findings on the incorporation of P32 into the lipid phosphorus and pentosenucleic acid of brain slices.

Download Full-text