Field comparison of the interferon-gamma assay and the intradermal tuberculin test for the diagnosis of bovine tuberculosis

Bovine tuberculosis was investigated in one private herd with 171 cattle after five cases were suspected to be tuberculous following post mortem examination. Using the intradermal comparative cervical tuberculin test 18 (10.5%) animals (ages from 2 to 12 years) were positive: 11 animals of N’dama breed and seven animals of White Fulani (i.e. Bunaji) breed; 17 female and one male animal. In all 11 randomly selected positive reactors, a spectrum of tuberculous lesions affecting the lungs, spleen, heart, liver, and the lymph nodes were observed. All the smear samples obtained were positive for acid-fast bacilli; cultural isolation confirmed the growth of mycobacteria on pyruvate-enriched Loewenstein-Jensen medium, which were identified by molecular typing to be Mycobacterium bovis. This study demonstrates widespread infection in this cattle herd and potential risk of infection for the human population with M. bovis.

Download Full-text

Evaluation of the McLintock syringe as a cause of non-specific reactions in the intradermal tuberculin test used for the diagnosis of bovine tuberculosis

Research in Veterinary Science ◽

10.1016/j.rvsc.2018.11.025 ◽

2019 ◽

Vol 122 ◽

pp. 175-178 ◽

Cited By ~ 2

Author(s):

A. Roy ◽

A. Díez-Guerrier ◽

J. Ortega ◽

M.L. de la Cruz ◽

J.L. Sáez ◽

...

Keyword(s):

Bovine Tuberculosis ◽

Tuberculin Test ◽

Specific Reactions

Download Full-text

Factors Affecting Herd Status for Bovine Tuberculosis in Dairy Cattle in Northern Thailand

Veterinary Medicine International ◽

10.1155/2017/2964389 ◽

2017 ◽

Vol 2017 ◽

pp. 1-6 ◽

Cited By ~ 1

Author(s):

Tawatchai Singhla ◽

Sukolrat Boonyayatra ◽

Veerasak Punyapornwithaya ◽

Kimberly L. VanderWaal ◽

Julio Alvarez ◽

...

Keyword(s):

Dairy Cows ◽

Bovine Tuberculosis ◽

Case Control Study ◽

Tuberculin Test ◽

Dairy Farms ◽

Case Control ◽

Chiang Mai ◽

Northern Thailand ◽

Factors Affecting ◽

Control Study

The objective of this case-control study was to identify farm-level risk factors associated with bovine tuberculosis (bTB) in dairy cows in northern Thailand. Spatial analysis was performed to identify geographical clustering of case-farms located in Chiang Mai and Chiang Rai provinces in northern Thailand. To identify management factors affecting bTB status, a matched case-control study was conducted with 20 case-farms and 38 control-farms. Case-farms were dairy farms with at least single intradermal tuberculin test- (SIT-) reactor(s) in the farms during 2011 to 2015. Control-farms were dairy farms with no SIT-reactors in the same period and located within 5 km from case-farms. Questionnaires were administered for data collection with questions based on epidemiological plausibility and characteristics of the local livestock industry. Data were analyzed using multiple logistic regressions. A significant geographic cluster was identified only in Chiang Mai province (p<0.05). The risk factor associated with presence of SIT-reactors in dairy herds located in this region was purchasing dairy cows from dealers (OR = 5.85, 95% CI = 1.66–20.58, and p=0.006). From this study, it was concluded that geographic clustering was identified for dairy farms with SIT-reactors in these provinces, and the cattle movements through cattle dealers increased the risks for SIT-reactor farm status.

Download Full-text

Prevalence of bovine tuberculosis in buffaloes in Hyderabad and Tando Allahyar districts of Sindh, Pakistan

Indian Journal of Animal Research ◽

10.18805/ijar.b-931 ◽

2018 ◽

Author(s):

Kanwar Kumar Malhi ◽

Asghar Ali Kamboh ◽

Chandar Kumar ◽

Prakash Dewani ◽

Mukesh Kumar ◽

...

Keyword(s):

Milk Yield ◽

Bovine Tuberculosis ◽

Tuberculin Test ◽

Herd Size ◽

Enzyme Linked Immunosorbent Assay ◽

Potential Hazard ◽

Culture Test ◽

Milk Samples ◽

Sindh Province ◽

Apparently Healthy

An investigation in buffaloes was carried out in Hyderabad and Tando Allahyar districts, Sindh province, Pakistan to determine the prevalence of bovine tuberculosis. The buffaloes (n=120) were first screened through single intradermal tuberculin test (SITT), then their sera were used for enzyme-linked immunosorbent assay (ELISA). Attempts were further made to isolate the Mycobacterium bovis organism from the milk samples using traditional culture test. Overall prevalence of 4.16%, 8.33% and 2.5% was recorded by SITT, ELISA and culture test respectively. A somewhat higher prevalence was recorded in Tando Allahyar district (SITT 6.66%; ELISA 10%; culture test 5%) as compared to Hyderabad district (SITT 1.66%; ELISA 6.66%; culture test 0%). Statistical analysis did not show any association (P> 0.05) of herd size, sex, age, milk yield and farming type with the prevalence of the disease. Whereas, SITT showed a significant (P Less than 0.05) association of sex, age and milk yield with the prevalence of the disease in Tando Allayer district. The results of present study revealed that bovine tuberculosis is present in apparently healthy buffalo herds of Hyderabad and Tando Allayer districts. Moreover, infected animals shed the M. bovis pathogen in milk that could be a potential hazard to public health.

Download Full-text

Diagnosis of Mycobacterium bovis infection in livestock using gamma interferon assay and single intradermal comparative tuberculin test in Assam and Meghalaya

Indian Journal of Animal Research ◽

10.18805/ijar.v0iof.7662 ◽

2017 ◽

Author(s):

Acheenta G. Barua ◽

Himangshu Raj ◽

Ashok Kumar ◽

Chandana C. Barua ◽

Arundhati Purkayastha ◽

...

Keyword(s):

Mycobacterium Bovis ◽

Bovine Tuberculosis ◽

Screening Method ◽

Tuberculin Test ◽

Gamma Interferon ◽

Tuberculosis Infection ◽

Specific Detection ◽

Biochemical Tests ◽

Diagnostic Potential ◽

Pcr Targeting

The present study was carried out to investigate the diagnostic potential of gamma interferon (IFN-ã) assay and single intradermal comparative tuberculin test (SICTT), including species specification of bovine tuberculosis infection in different livestock farms of Assam and Meghalaya. A total of 199 animals (cattle and buffalo) were examined for bovine tuberculosis symptoms and swab samples were cultured. Biochemical tests and PCR were used for species specification of bovine tuberculosis. Out of 199 cases examined, 33 (16.58%) showed positive for SICTT, 39 (19.59%) for IFN-ã and 35(17.59%) for PCR. Based on PCR targeting pncA region, the confirmation was done for M. Bovis. IFN-ã thus ensures a sensitive and specific detection of early bovine tuberculosis infection together with SICTT and hence may be considered as a screening method of choice.

Download Full-text