Inactivation of Photosensitizing Merocyanine Dyes by Plasma, Serum and Serum Components

SummaryIn order to investigate the possibility that autoantibodies to thrombomodulin (TM) may exist in patients with the lupus anticoagulant (LA) and perhaps be implicated in the pathogenesis of recurrent thrombosis seen in such patients, we developed an enzyme-immunoassay to screen serum samples for anti-human TM activity. The major technical problem encountered in developing this assay was to reduce the non-specific binding of serum components from both the LA positive and the negative population. Considerable reduction of non-specific binding was achieved by use of a phosphate/citrate buffer at pH 8.0 and the use of an optimal sample dilution of 1/40. In addition, samples were always tested in parallel in blank wells and results are expressed as an OD ratio. Samples from 113 patients with the LA were assayed and compared to 78 patients referred for LA testing but found to be negative. The mean OD values for the LA positive patients (± SD) was 1.36 (0.44) with a range of 0.78-2.57. This was virtually identical to the values for the LA negative population (1.38 ± 0.40, range 0.76-2.77). The results of this study indicate that there is no evidence for the presence of a significant autoantibody activity to TM in patients with the LA when compared to LA negative patients. If such autoantibodies do exist their frequency must be quite low.

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Experimental basis of standardized specimen collection: The effect of moderate ethanol consumption on some serum components (K, Na, ASAT, ALAT, CK, LD, total protein)

Scandinavian Journal of Clinical and Laboratory Investigation ◽

10.3109/00365518709168911 ◽

1987 ◽

Vol 47 (4) ◽

pp. 337-343 ◽

Cited By ~ 1

Author(s):

E. A. Leppanen ◽

R. Grasbeck

Keyword(s):

Total Protein ◽

Ethanol Consumption ◽

Experimental Basis ◽

Specimen Collection ◽

Serum Components

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Synthesis, photosensitization and antimicrobial activity evaluation of some novel Merocyanine dyes

10.31221/osf.io/yv273 ◽

2019 ◽

Author(s):

Chem Int

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Aspergillus Flavus ◽

Elemental Analysis ◽

Cyanine Dyes ◽

Fungal Strains ◽

Activity Evaluation ◽

Visible Spectra ◽

Merocyanine Dyes

Novel acyclic and cyclic merocyanine dyes derived from the nucleu of furo [(3,2-d) pyrazole; ( d 2 , 3 )imidazole]were prepared. The electronic visible absorptionspectra of all the synthesized new cyanine dyes were examined in 95% ethanolsolution to evaluate their photosensitization properties. Antibacterial andantifungal activities for some selected dyes were tested against various bacterialand fungal strains (Escherichia coli, Staphylococcus aureus, Aspergillus flavus andCandida albicans) to evaluate their antimicrobial activity. Structural identificationwas carried out via elemental analysis, visible spectra, IR and 1H NMRspectroscopic data.

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Effect of Saengmaec-san on the Level of Blood Glucose and Serum Components in Streptozocin-Induced Diabetic Rats

Journal of the Korean Society of Food Science and Nutrition ◽

10.3746/jkfn.2009.38.9.1179 ◽

2009 ◽

Vol 38 (9) ◽

pp. 1179-1186 ◽

Cited By ~ 2

Author(s):

Hyeong-Jin Kim ◽

Hye-Jin Yang ◽

Mi-Hwan Kim ◽

Gi-Hyung Ryu ◽

Ji-Youn Jung

Keyword(s):

Blood Glucose ◽

Diabetic Rats ◽

Serum Components

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Merocyanine dyes

Physical Sciences Reviews ◽

10.1515/psr-2020-0145 ◽

2021 ◽

Vol 0 (0) ◽

Cited By ~ 4

Author(s):

Heinz Mustroph

Keyword(s):

Electronic Structure ◽

Fluorescent Dyes ◽

Silver Halide ◽

Equilibrium Structure ◽

Polymethine Dyes ◽

Spectral Sensitization ◽

Technical Application ◽

Textile Dyeing ◽

Merocyanine Dyes ◽

Active Methylene

Abstract Merocyanine dyes belong to the class of neutral polymethine dyes, where one terminal component is typically found in cyanine dyes and the second obtained from an active methylene compound. The different electron acceptor/donator abilities of the two terminal components have a marked impact on the electronic structure of a merocyanine dye and its equilibrium structure and electronic spectra. Their first technical application was spectral sensitization in silver halide photography. Today they have numerous of applications in textile dyeing and as membrane potential sensitive fluorescent dyes.

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