scholarly journals Dynamic of active microorganisms inhabiting a bioleaching industrial heap of low-grade copper sulfide ore monitored by real-time PCR and oligonucleotide prokaryotic acidophile microarray

2009 ◽  
Vol 2 (6) ◽  
pp. 613-624 ◽  
Author(s):  
Francisco Remonsellez ◽  
Felipe Galleguillos ◽  
Mercedes Moreno-Paz ◽  
Víctor Parro ◽  
Mauricio Acosta ◽  
...  
2013 ◽  
Vol 825 ◽  
pp. 396-400 ◽  
Author(s):  
Bo Wei Chen ◽  
Liu Lu Cai ◽  
Biao Wu ◽  
Xue Liu ◽  
Jian Kang Wen

Shake flask bioleaching of a low-grade nickel-cobalt-copper sulfide ore from Lao was investigated to test the technical feasibility to recovery metals from this ore. The mineralogical results indicated that the valuable minerals were disseminated finely and the ore had a low content of sulfur and high content of iron and magnesium, which was very harmful for bioleaching. The effects of different parameters such as the amount of acid addition, inoculation volume, initial pH, temperature and leaching time on the bioleaching performance of the ore were evaluated after pre-leaching with dilute acid. The results showed that nickel, cobalt, copper leaching rates reached 81.61%, 80.75%, 70.97% at 33 °C and 83.40%, 82.13%, 70.34% at 45 °C. The mineralogical analysis of the leaching residues revealed that the leaching rate of nickel sulfide was more than 92% and the unleached nickel was mainly present as nickel silicate.


2014 ◽  
Vol 58 ◽  
pp. 39-51 ◽  
Author(s):  
H.R. Watling ◽  
D.W. Shiers ◽  
J. Li ◽  
N.M. Chapman ◽  
G.B. Douglas

2020 ◽  
Vol 27 (5) ◽  
pp. 1404-1415
Author(s):  
Chen-bing Ai ◽  
Yu-ting Liang ◽  
Guan-zhou Qiu ◽  
Wei-min Zeng

2012 ◽  
Vol 581-582 ◽  
pp. 851-855
Author(s):  
Rong Dong Deng ◽  
Quan Jun Liu ◽  
Xiao Feng Yang ◽  
Ting Hu ◽  
Feng Hong Ye

Through the processing of mineralogical study on a copper ore from Jilin province in China, it found that the copper exist mostly with the form of chalcopyrite, and others exist with the form of copper oxide minerals which with complex ingredient. The copper oxide minerals are due to the altered of the edge of chalcopyrite, and have not formed the independent copper oxide ore. It belongs to unconventional hard-to-beneficiate copper ore. Coarse grinding fineness and bulk floatation was used to recover the copper oxide ore and copper sulfide ore by using amino double phosphoric acid and butylamine dithiophosphate as the collector. This process was similar to the carrier flotation and achieved an effective processing of the copper oxide ore and obtained a high-grade copper concentrate assaying 15.8% with a high recovery of 82.4%.


2007 ◽  
Vol 20-21 ◽  
pp. 539-542 ◽  
Author(s):  
Francisco Remonsellez ◽  
F. Galleguillos ◽  
Sonestie Janse van Rensburg ◽  
G.F. Rautenbach ◽  
Pedro A. Galleguillos ◽  
...  

Microbial heap bioleaching is being used as an industrial process to recover copper from low grade ores. It is known that a consortium of different microorganisms participates in this process. Therefore identification and quantification of communities inhabiting heap bioleaching operations is a key step for understanding the dynamics and role of these microorganisms in the process. A quantitative real-time PCR approach was used to investigate the microbial dynamics in this process. To study the microbial population inhabiting a low-grade copper sulphide ore bioleaching industrial heap process at Escondida Mine in Chile, 16S rRNA genetic libraries were constructed using bacterial and archaeal universal primers. Phylogenetic analyses of sequences retrieved from genetic libraries showed that the community is mainly composed by microoganisms related to Acidithiobacillus ferrooxidans (2 strains), Acidithiobacillus thiooxidans, Leptospirillum ferrooxidans, Leptospirillum ferriphilum and the archaea Ferroplasma. Specific primers for real-time PCR determination were designed and tested to amplify each of the sequences obtained by cloning. Standard curves for real time PCR were performed using plasmid DNA from selected clones. This methodology is actually being used to monitor relevant microorganisms inhabiting this low-grade copper sulphide ore bioleaching industrial heap.


2016 ◽  
Vol 164 ◽  
pp. 355-361 ◽  
Author(s):  
Yan Jia ◽  
Heyun Sun ◽  
Defang Chen ◽  
Hongshan Gao ◽  
Renman Ruan

2014 ◽  
Vol 522-524 ◽  
pp. 1471-1475
Author(s):  
Tie Min Zhang ◽  
Jian Jun Fang ◽  
Tai Guo Jiang ◽  
Shan Wang ◽  
Ying Bo Mao

Floatation experimental research is carried out on a certain complicated ore containing mixed sulphides in Yannan province. For the characteristics of the mine and the scene of the test requirements, the use of mixed flotation-separation process has achieved a successful recovery of useful elements. Combination inhibitors can effectively achieve the separation of copper and sulfur .Under the proper process conditions ,the good technical indexes were obtained.


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