scholarly journals Isolation and identification of bacteriocinogenic strain of Lactobacillus plantarum with potential beneficial properties from donkey milk

2013 ◽  
Vol 114 (6) ◽  
pp. 1793-1809 ◽  
Author(s):  
A. Murua ◽  
S.D. Todorov ◽  
A.D.S. Vieira ◽  
R.C.R. Martinez ◽  
A. Cencič ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Donkey Milk ◽  
Isolation And Identification

scholarly journals ISOLATION AND IDENTIFICATION OF LACTIC ACID BACTERIA (LAB) PRODUCING BACTERIOSIN-LIKE FROM SMOKED GIANT CATFISH (Arius thalassinus)

2021 ◽  
Vol 2 (3) ◽  
pp. 170-178
Author(s):  
Tita Rialita ◽  
Sumanti Debby Moody ◽  
Edy Subroto ◽  
Heditia Febby Susanto
Keyword(s):  
Antimicrobial Activity ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Lactobacillus Plantarum ◽  
Ethylenediaminetetraacetic Acid ◽  
Sodium Dodecyl ◽  
Isolation And Identification ◽  
Smoked Fish ◽  
Giant Catfish ◽  
Strong Antimicrobial Activity

Bacteriocin was bacterial metabolite that have antimicrobial properties, so it had the potential to be used as food bio preservatives. Bacteriocin was produced by lactic acid bacteria (LAB), one of the sources of which was from smoked fish products. Some regions in Indonesia produce various types of smoked fish from various types of fish, which were thought to contain bacteriocin-producing lactic acid; one of them was giant cathfish (Arius thalassinus). This study aims to obtain LAB isolates that have strong antimicrobial activity and have the potential to produce bacteriocin-like from smoked giant catfish (Arius thalassinus).  The research method used an experimental method that analyzed descriptively.  Based on the results, there were 15 isolates LAB isolated from smoked giant catfish. Three selected isolates showed strong antimicrobial activity inhibiting E. coli and S. aureus bacteria, and the most effective inhibiting Salmonella sp. One selected LAB isolates identified Pediococcus acidilactici suspected to produce pediocin bacteriocin-like, while the other two isolates identified Lactobacillus plantarum sp 1 and Lactobacillus plantarum sp 2 which suspected to produce plantaricin bacteriocin. Bacteriocin from the three isolates of LAB had characteristics stable to temperatures up to 121oC, stable in pH range 2-6, and bacteriocin activity increased with the addition of SDS (Sodium Dodecyl Sulfate) and EDTA (Ethylenediaminetetraacetic acid) surfactants. The conclusion was that the bacteriocin produced was stable at high temperature, low pH, and resistence in the presence of surfactants, so it had the potential to be developed as biopreservatives material in preserving fish-based foods

scholarly journals Isolation and Identification of Lactobacillus plantarum HFY05 from Natural Fermented Yak Yogurt and Its Effect on Alcoholic Liver Injury in Mice

2019 ◽  
Vol 7 (11) ◽  
pp. 530 ◽  
Author(s):  
Ruokun Yi ◽  
Fang Tan ◽  
Wei Liao ◽  
Qiang Wang ◽  
Jianfei Mu ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Liver Injury ◽  
Lactobacillus Plantarum ◽  
Quantitative Polymerase Chain Reaction ◽  
Lactobacillus Delbrueckii ◽  
Tibet Plateau ◽  
Alcoholic Liver Injury ◽  
Isolation And Identification ◽  
Oxide Synthase

Yak yogurt is a type of naturally fermented dairy product prepared by herdsmen in the Qinghai-Tibet Plateau, which is rich in microorganisms. In this study, a strain of Lactobacillus plantarum was isolated and identified from yak yogurt in Hongyuan, Sichuan Province and named Lactobacillus plantarum HFY05 (LP-HFY05). LP-HFY05 was compared with a common commercial strain of Lactobacillus delbrueckii subsp. bulgaricus (LDSB). LP-HFY05 showed better anti-artificial gastric acid and bile salt effects than LDSB in in vitro experiments, indicating its potential as a probiotic. In animal experiments, long-term alcohol gavage induced alcoholic liver injury. LP-HFY05 effectively reduced the liver index of mice with liver injury, downregulated the levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, triglyceride, total cholesterol, blood urea nitrogen, nitric oxide, and MDA and upregulated the levels of albumin, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase in the serum of liver-injured mice. LP-HFY05 also reduced the levels of interleukin (IL)-6, IL-12, tumor necrosis factor-alpha, and interferon-gamma in the serum of liver-injured mice. The pathological observations showed that LP-HFY05 reduced the damage to liver cells caused by alcohol. Quantitative polymerase chain reaction and Western blot assays further showed that LP-HFY05 upregulated neuronal nitric oxide synthase, endothelial nitric oxide synthase, manganese-SOD, cuprozinc-SOD, CAT, and inhibitor of κB-α mRNA and protein expression and downregulated the expression of nuclear factor-κB-p65 and inducible nitric oxide synthase in the livers of liver-injured mice. A fecal analysis revealed that LP-HFY05 regulated the microbial content in the intestinal tract of mice with liver injury, increased the content of beneficial bacteria, including Bacteroides, Bifidobacterium, and Lactobacillus and reduced the content of harmful bacteria, including Firmicutes, Actinobacteria, Proteobacteria, and Enterobacteriaceae, thus, regulating intestinal microorganisms to protect against liver injury. The effect of LP-HFY05 on liver-injured mice was better than that of LDSB, and the effect was similar to that of silymarin. LP-HFY05 is a high-quality microbial strain with a liver protective effect on experimental mice with alcoholic liver injury.

scholarly journals Isolation and Identification of Bacteriocin-Producing Lactic Acid Bacteria from Urutan, Balinese Traditional Fermented sausage

2021 ◽  
Vol 8 (1) ◽  
pp. 81
Author(s):  
Ni Made Sri Dwijastuti ◽  
I Nengah Sujaya ◽  
Ni Nengah Dwi Fatmawati
Keyword(s):  
Escherichia Coli ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Lactobacillus Plantarum ◽  
Enterococcus Faecalis ◽  
Indicator Bacteria ◽  
Isolation And Identification ◽  
16S Rdna Gene ◽  
Fermented Sausage ◽  
Inhibition Zones

This study aimed to isolate and identify lactic acid bacteria (LAB) that could produce bacteriocins from urutan. Urutan is a Balinese sausage that is traditionally produced and naturally fermented. Urutans used in this study were obtained from several traders in the Baturiti, Mengwi, and Tegallalang region, Bali. More than 300 LAB colonies were isolated and underwent inhibition test against the growth of the indicator bacteria. Crude bacteriocins from two isolates, namely J2 and J6, showed inhibition zones against the growth of Lactobacillus plantarum E12.1 and Escherichia coli ATCC 8739. The J6 isolate also inhibited the growth of Enterococcus faecalis ATCC 29212. Both J2 and J6 were gram-positive, bacilli shaped, and showed negative catalase test. Based on the results of the 16S rDNA gene amplification, the two isolates were identified as Lactobacillus plantarum. Keywords: Lactic Acid Bacteria, Bacteriocin, Lactobacillus plantarum, Urutan

Isolation and Identification of Lactobacillus casei and Lactobacillus plantarum from Plants by PCR and Detection of their Antibacterial Activity

2009 ◽  
Vol 9 (8) ◽  
pp. 810-814 ◽  
Author(s):  
M. Amin ◽  
M. Jorfi ◽  
A.D. Khosravi ◽  
A.R. Samarbafza ◽  
A. Farajzadeh Sheikh
Keyword(s):  
Antibacterial Activity ◽  
Lactobacillus Plantarum ◽  
Lactobacillus Casei ◽  
Isolation And Identification

Lactobacillus plantarum and Streptococcus thermophilus as starter cultures for a donkey milk fermented beverage

2017 ◽  
Vol 256 ◽  
pp. 54-61 ◽  
Author(s):  
Barbara Turchi ◽  
Francesca Pedonese ◽  
Beatrice Torracca ◽  
Filippo Fratini ◽  
Simone Mancini ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Streptococcus Thermophilus ◽  
Starter Cultures ◽  
Donkey Milk ◽  
Fermented Beverage

scholarly journals Isolation and identification of local isolates of Lactobacillus plantarum and evaluation of their efficacy in controlling tomato Fusarium wilt disease

2020 ◽  
Vol 38 (2) ◽  
pp. 149-161
Author(s):  
Abdallah Hassan ◽  
◽  
Abeer Mahmoud ◽  
Lina Mohammad ◽  
◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Fusarium Wilt ◽  
Wilt Disease ◽  
Isolation And Identification ◽  
Fusarium Wilt Disease
Sign in / Sign up

Export Citation Format

Share Document