scholarly journals Isolation and Characterization of Brassicasterol from N-Hexane Fraction of Pometia pinnata and its Toxicity Test

2021 ◽  
Vol 10 (9) ◽  
pp. 207-215
Author(s):  
Yoan De Nanda Herru Adlis Santoni ◽  
Mai Efdi
Keyword(s):  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Secondary Metabolite ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Hexane Fraction ◽  
Spectroscopic Methods ◽  
Brine Shrimp Lethality ◽  
Isolation And Characterization

Pometia pinnata leaves were extracted and fractionated using n-hexane, dichloromethane, ethyl acetate, and methanol. The four fractions obtained were screened for cytotoxic testing using the Brine shrimp lethality test (BSLT) method, n-hexane fraction has the highest LC50 419,855 mg/L.The n-hexane fraction was continued for the isolation stage and a secondary metabolite compound was obtained, namely brassicasterol. The structure of this secondary metabolites was determined using spectroscopic methods (UV-Vis, FTIR, and NMR).

scholarly journals ANALISIS SENYAWA METABOLIT SEKUNDER DAN UJI TOKSISITAS EKSTRAK ETANOL BATANG TANAMAN PATAH TULANG (Euphorbia tirucalli L.) DENGAN METODE Brine Shrimp Lethality Test (BSLT)

2014 ◽  
Vol 14 (2) ◽  
pp. 106 ◽  
Author(s):  
Grace S. Baud ◽  
Meiske S. Sangi ◽  
Harry S.J. Koleangan
Keyword(s):  
Secondary Metabolite ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Phytochemical Screening ◽  
Brine Shrimp Lethality ◽  
Euphorbia Tirucalli ◽  
Ethanol Extracts

ABSTRAK Telah dilakukan penelitian mengenai analisis senyawa metabolit sekunder dan uji toksisitas pada batang tanaman Patah tulang (Euphorbia tirucalli L.). Analisis senyawa metabolit sekunder dilakukan dengan skrining fitokimia yaitu senyawa alkaloid, flavonoid, triterpenoid, steroid, saponin dan tanin pada ekstrak segar dan kering batang tanaman Patah tulang. Penentuan toksisitas ekstrak etanol batang tanaman Patah tulang menggunakan metode Brine Shrimp Lethality Test (BSLT). Uji toksisitas digunakan hewan uji Artemia salina Leach sebagai bioindikator. Pada skrining fitokimia diperoleh positif flavonoid, tanin dan steroid sedangkan alkaloid, saponin dan triterpenoid negatif. Data pengujian toksisitas diperoleh dari analisis Lethality Concentration 50 (LC50) yang dilakukan dengan metode probit menggunakan perangkat lunak SPSS 20.0 (untuk sistem operasi Windows). Hasil uji toksisitas ekstrak etanol batang Patah tulang menunjukkan bahwa ekstrak segar maupun kering bersifat sitotoksik dengan nilai LC50 (7,994ppm) untuk ekstrak etanol batang segar  dan LC50 (9,940ppm) untuk ekstrak etanol batang kering. Kata kunci : Euphorbia tirucalli L., Senyawa metabolit sekunder, Toksisitas, Artemia salina Leach, LC50.   ANALYSIS OF SECONDARY METABOLITE COMPOUNDS AND TOXICITY TEST OF  STEM  PLANT ETHANOL EXTRACTS OF  PATAH TULANG (Euphorbia tirucalli L.) BY BRINE SHRIMP LETHALITY TEST’S METHOD (BSLT) ABSTRACT A research on the analysis of secondary metabolite compounds contained in the stem of  patah tulang (Euphorbia tirucalli L.) and its toxicity had been done. The analysis was performed through phytochemical screening of alkaloids, flavonoids, triterpenoids, steroids, saponins and tannins on extracts of fresh and dried stem of the plant. Toxicity of ethanol extract of the stem was determined using Brine Shrimp Lethality Test (BSLT) method with Artemia salina Leach as bioindicator. Phytochemical screening showed positive results for flavonoids, tannins, and steroids and negative one for alkaloids, saponins, and triterpenoids. Toxicity data were obtained from Lethality Concentration 50 (LC50) calculation trhrough probit analysis using SPSS 20.0 software (for Windows operating systems). Toxicity test showed that ethanol extracts of the fresh and dried stem were cytotoxic with LC50 value of 7.994 ppm and 9.940 ppm, respectively. Keywords: Euphorbia tirucalli L., Secondary metabolite compounds, Toxicity, Artemia salina Leach, LC50.

scholarly journals Characterization of in-vitro antioxidant, cytotoxic, thrombolytic and membrane stabilizing potential of different extracts of Cheilanthes tenuifolia and Stigmasterol isolation from n-hexane extract

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Ananna Mahfuz ◽  
Fayad Bin Abdus Salam ◽  
Kanij Nahar Deepa ◽  
A. H. M. Nazmul Hasan
Keyword(s):  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Column Chromatography ◽  
Hexane Fraction ◽  
Chloroform Fraction ◽  
Potential Candidate ◽  
Methanol Fraction ◽  
H Nmr ◽  
Isolation And Characterization

Abstract Background Cheilanthes tenuifolia, a member of the Pteridaceae family, is an evergreen and small fern could be abundant of bioactive compounds. The present study was designed to investigate its many therapeutic properties and isolation of bioactive compounds from extracts of Cheilanthes tenuifolia. Methods The dried coarse plant powder was extracted with methanol and dried with rotary evaporator. The extract was further partitioned according to the increasing polarity: N-hexane < chloroform < ethyl-acetate < methanol by modified Kupchan method. Then each extract fractions were investigated for their pharmacologic properties. Compounds were isolated from n-hexane fraction through column chromatography, followed by TLC and structure was determined by analysis of sample using 1H-NMR and matched with published phytochemistry report. Results Methanol fraction of Cheilanthes tenuifolia showed highest amount of total phenol content (11.32 ± 0.28 mg/gm GAE) followed by chloroform fraction (9.71 ± 0.31 mg/gm GAE) > N-hexane fraction (6.69 ± 0.67 mg/gm GAE) > ethyl acetate fraction (5.36 ± 0.54 mg/gm GAE). The methanol fraction of Cheilanthes tenuifolia possessed highest amount (7.11 ± 0.52 mg/gm QE) of total flavonoid content. Our present study indicates that methanol extract was most potent (IC50 = 9.926 μg/ml) inhibitor of DPPH free radicals. In brine shrimp lethality bio assay, all the extracts showed dose dependent increment of mortality and chloroform extract was found most cytotoxic (LC50 = 34.493 μg/ml) compared to other plant extracts. The chloroform fraction of Cheilanthes tenuifolia was most potent in terms of thrombolytic activity. A compound was isolated (CT-2) using column chromatography followed by TCL and PTLC (35% pet ether in CHCl3) and analyzed by 1H-NMR. The structure of stigmasterol was confirmed by comparing the 1H-NMR data with previously published phytochemistry report. Conclusion Cheilanthes tenuifolia could be a potential candidate for bioactive compounds and further studies on isolation and characterization of its bioactive compounds are highly required.

scholarly journals Kajian Toksisitas dari Fraksi Heksana, Etil Asetat, dan Etanol Daun Soyogik (Sauraia bracteosa DC)

Jurnal MIPA ◽  
2015 ◽  
Vol 4 (2) ◽  
pp. 40
Author(s):  
Triyono Mojo ◽  
Jemmy Abidjulu ◽  
Max R. J. Runtuwene
Keyword(s):  
Ethyl Acetate ◽  
Brine Shrimp ◽  
Toxicity Testing ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Toxicity Tests ◽  
Brine Shrimp Lethality ◽  
Lc50 Value

Telah dilakukan penelitian mengenai Kajian Toksisitas dari Fraksi Heksana, Etil Asetat dan Etanol Daun Soyogik (Saurauia bracteosa DC). Daun Soyogik dikeringanginkan sampai kering dan dimaserasi berturut-turut menggunakan pelarut heksana, etil asetat dan etanol. Uji toksisitas dilakukan  menggunakan metode Brine Shrimp Lethality Test (BSLT), kemudian diidentifikasi presentasi kematian larva udang menggunakan analisis probit (LC50). Hasil pengujian toksisitas menunjukkan nilai LC50 yaitu : fraksi  heksana (181,97), fraksi etil asetat (12,97) dan fraksi etanol (2,82).A research has been about Toxicity  of the Fraction Soyogik Leaves (Saurauia bracteosa DC) from Hexane, Ethyl Acetate and Ethanol. Soyogik leaves drying till dunes and maceratied successively using hexane, ethyl acetate and ethanol solvent. Toxicity tests performed using Brine Shrimp Lethality Test (BSLT) methode. Toxicity testing showed the LC50 value is hexane fraction (181,97), ethyl acetate fraction (12,97) and fraction of ethanol (2,82).

scholarly journals Skrining Fitokimia Dan Uji Toksisitas Ekstrak Biji Aren (Arenga pinnata MERR.)

Jurnal MIPA ◽  
2017 ◽  
Vol 6 (2) ◽  
pp. 12 ◽  
Author(s):  
Dhea Amelia Arief ◽  
Meiske Sangi ◽  
Vanda S. Kamu
Keyword(s):  
Regression Analysis ◽  
Secondary Metabolites ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Triterpenoid Saponin ◽  
Phytochemical Screening ◽  
Brine Shrimp Lethality ◽  
Palm Sugar

Penelitian ini bertujuan untuk melakukan skrining fitokimia dari Biji Aren (Arenga pinnata Merr.) dan menentukan toksisitas dengan menggunakan metode Brine Shrimp Lethality Test (BSLT). Ekstrak etanol dari biji aren mengandung beberapa senyawa metabolit sekunder seperti flavonoid, triterpenoid, saponin dan tanin sebagai hasil uji fitokimia. Uji toksisitas terhadap indikator larva udang Artemia salina Leach. Menunjukkan nilai LC50 bersifat toksik. Nilai LC50 yang diperoleh sebesar 50, 64 ppm yang ditentukan dengan analisis regresi menggunakan MS Office Excel 2007The research aimed to recognize the phytochemical screening and to determine the toxicity on sugar palm (Arenga pinnata Merr.) seed using Brine Shrimp Lethality Test (BSLT) . Ethanol extract of palm sugar seed contained some compounds secondary metabolites such as flavonoid, triterpenoid, saponin, and tannin as a result of phytochemical test. The toxicity test against animal indicator shrimp Artemia salina Leach showed LC50 values were obtained at 50,64 ppm determined by regression analysis using MS Office Excel 2007.

scholarly journals TOKSISITAS SENYAWA Β-SITOSTEROL DAN EKSTRAK KULIT BATANG AGLAIA

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Riky Riky
Keyword(s):  
Ethyl Acetate ◽  
Toxic Effect ◽  
Traditional Medicine ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Test Method ◽  
Natural Materials ◽  
Brine Shrimp Lethality ◽  
Methanol Extracts ◽  
Isolated Compound

Pacar cina (Aglaia odorata Lour) is a plant that is widely used as an traditional medicine because of natural materials they contain. The  study  aims  to  from Pacar cina extracts and toxicity test of each extract and isolated compound by BSLT (Brine Shrimp Lethality Test) method. The method used in the maceration. In this study, the n-hexane, ethyl acetate, methanol extracts and β-sitosterol compound showed toxic effect (LC50 261,17; 110; 266,75 and 100 μg/mL, respectively). The toxicity degree among the three extracts and isolated compound is β-sitosterol > ethyl acetate > n-hexane > methanol.

scholarly journals Isolation and Characterization of 2,4,5-Trimethoxy-benzaldehyde and 1-(2,4,5-trimethoxyphenyl)-Ethanone from Pachypodanthium Staudtii, and their General Toxicity towards Brine Shrimps

2006 ◽  
Vol 1 (5) ◽  
pp. 1934578X0600100
Author(s):  
Lutfun Nahar ◽  
Satyajit D. Sarker
Keyword(s):  
Brine Shrimp ◽  
2D Nmr ◽  
Stem Bark ◽  
Benzene Derivatives ◽  
Brine Shrimp Lethality ◽  
Isolation And Characterization ◽  
General Toxicity ◽  
Brine Shrimps

The 1D and 2D NMR analyses, notably, 1H, 13C PENDANT, HMQC, HMBC and NOESY, were carried out on two benzene derivatives, 2,4,5-trimethoxybenzaldehyde and 1-(2,4,5-trimethoxyphenyl)-ethanone, isolated from the dichloromethane extract of the stem bark of Pachypodanthium staudtii. The general toxicity of these compounds (LC50 = 32.6 and 80.5 μg/mL, respectively) was determined by the brine shrimp lethality bioassay.

scholarly journals Isolation of sea cucumbers’s (Holothuria atra) secondary metabolite using column-chromatography technique

2021 ◽  
Vol 869 (1) ◽  
pp. 012010
Author(s):  
S Agustina ◽  
S Bella ◽  
S Karina ◽  
I Irwan ◽  
M Ulfah
Keyword(s):  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Secondary Metabolite ◽  
Column Chromatography ◽  
Extraction Method ◽  
Sea Cucumber ◽  
Sea Cucumbers ◽  
Marine Chemistry ◽  
Holothuria Atra ◽  
Metabolite Content

Abstract Identification of sea cucumbers from Benteng Inong Balee, Aceh Besar and their phytochemistry screening were conducted in December 2020 to January 2021 at Laboratory of Marine Chemistry and Fisheries Biotechnology, Universitas Syiah Kuala. The purpose of this study was to identify the species of sea cucumbers and its secondary metabolite content using phytochemistry screening and column chromatography. The species of sea cucumbers that were identified was Holothuria atra. The extraction method used in sea cucumber extraction was maceration method, while the separation of secondary metabolites used column-chromatography with eluent of n-hexane : ethyl acetate (8:4). The results showed that secondary metabolites obtained from phytochemical tests were flavonoids, saponins and triterpenoids.

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