Proteomic analysis of an Enterococcus faecalis mutant generated against the exposure to silver nanoparticles

2021 ◽  
Author(s):  
Marco Felipe Salas‐Orozco ◽  
Nereyda Niño‐Martínez ◽  
Gabriel‐Alejandro Martínez‐Castañón ◽  
Fernando Torres Méndez ◽  
Gabriela Margarita Montero Morán ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Enterococcus Faecalis ◽  
Proteomic Analysis

scholarly journals Antibacterial efficacy of biosynthesized silver nanoparticles against Enterococcus faecalis Biofilm: An in vitro study

2018 ◽  
Vol 9 (2) ◽  
pp. 237 ◽  
Author(s):  
KiranRahul Halkai ◽  
JayashreeA Mudda ◽  
Vasundhara Shivanna ◽  
Vandana Rathod ◽  
Rahul Halkai
Keyword(s):  
Silver Nanoparticles ◽  
Enterococcus Faecalis ◽  
In Vitro Study ◽  
Vitro Study ◽  
Antibacterial Efficacy

scholarly journals Antibacterial Effects of Erbium Chromium Laser along with/without Silver Nanoparticles in Root Canals Infected by Enterococcus faecalis

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Seyedeh Sareh Hendi ◽  
Negin Amiri ◽  
Banafsheh Poormoradi ◽  
Mohammad Yousef Alikhani ◽  
Saeid Afshar ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Silver Nanoparticle ◽  
Colony Count ◽  
Antibacterial Effects ◽  
Bacterial Colony ◽  
Root Canals ◽  
Significant Difference ◽  
Root Canal System

This study investigates the antibacterial effects of erbium chromium laser at 2780 nm, silver nanoparticles, and erbium chromium along with silver nanoparticles on Enterococcus faecalis in comparison with sodium hypochlorite. In the present study, 90 extracted human single-rooted teeth were selected and standardized to a length of 15 mm. The canals were prepared by V-taper Gold rotary files and then incubated with E. faecalis for 21 days. The samples were divided into four experimental groups including hypochlorite sodium, silver nanoparticle, erbium chromium laser, and erbium chromium laser along with silver nanoparticle groups. Results showed that there was a significant reduction in colony count for all groups after interventions. Moreover, there was a significant reduction in the colony count for sodium hypochlorite group in comparison with another groups, and this group showed the highest reduction of colony count. There was a significant difference between silver nanoparticles and erbium chromium laser groups in colony count. According to the results, the silver nanoparticles offered strong antibacterial effects on E. faecalis and therefore can decrease bacterial colonies, while the use of the laser, despite the reduction of the bacterial colony, could not be sufficiently used for disinfection of root canal system.

scholarly journals Antimicrobial activity of endodontic sealers and medications containing chitosan and silver nanoparticles against Enterococcus faecalis

2019 ◽  
Vol 17 (3) ◽  
pp. 228080001985177 ◽  
Author(s):  
Juan Pablo Loyola-Rodríguez ◽  
Fernando Torres-Méndez ◽  
León Francisco Espinosa-Cristobal ◽  
José Obed García-Cortes ◽  
Alejandra Loyola-Leyva ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Zeta Potential ◽  
Enterococcus Faecalis ◽  
Membrane Surface ◽  
Chemical Components ◽  
Collagen Membrane ◽  
Ah Plus ◽  
Endodontic Sealers ◽  
Antimicrobial Effectiveness

Background: The main microorganism associated with the failure of endodontic treatments is Enterococcus faecalis. Although several endodontic therapeutics have demonstrated antimicrobial activity against E. faecalis, the antimicrobial effectiveness of chitosan (CsNPs) and silver nanoparticles (AgNPs) included into conventional endodontic sealers for endodontic therapies is still unclear. Aim: The objective of this study was to evaluate the antibacterial activity increment (AAI) of endodontic sealers containing CsNPs and AgNPs as well as some chemical components against E. faecalis by direct contact assays. Methods: CsNPs and AgNPs were synthesized by reduction and ionic gelation methods, respectively. Nanoparticles were characterized by dynamic light scattering and energy dispersive X-ray analysis. The bactericidal activity was tested on monolayers on agar plates and collagen membrane surface assays against E. faecalis. Results: The size of CsNPs was 70.6±14.8 nm and zeta potential was 52.0±5.4 mV; the size of AgNPs was 54.2±8.5 nm, and zeta potential was –48.4±6.9 mV. All materials, single or combined, showed an AAI, especially when CsNPs, chlorhexidine (Chx), and the combination of CsNPs-Chx were added. However, the combination of CsNPs-Chx showed the highest (55%) AAI, followed by Chx (35.5%) and CsNPs (11.1%), respectively. There was a significant statistical difference in all comparisons ( p < 0.05). Tubliseal (40%) and AH Plus (32%) sealants showed a higher AAI on E. faecalis in the monolayer test and collagen membrane assay analyzed by scanning electron microscopy. Conclusions: Tubliseal and AH plus sealers combined with nanoparticles, especially CsNPs-Chx, could be used for conventional endodontic treatments in the control of E. faecalis bacteria.

Proteomic analysis of soybean root exposed to varying sizes of silver nanoparticles under flooding stress

2016 ◽  
Vol 148 ◽  
pp. 113-125 ◽  
Author(s):  
Ghazala Mustafa ◽  
Katsumi Sakata ◽  
Setsuko Komatsu
Keyword(s):  
Silver Nanoparticles ◽  
Proteomic Analysis ◽  
Soybean Root ◽  
Flooding Stress

Antibacterial effect of silver nanoparticles against Enterococcus faecalis

2017 ◽  
Vol 7 (1) ◽  
pp. 29 ◽  
Author(s):  
AbdelhamiedYousef Saad ◽  
ZainabAli Alabdulmohsen
Keyword(s):  
Silver Nanoparticles ◽  
Enterococcus Faecalis ◽  
Antibacterial Effect

Combined Effect of a Mixture of Silver Nanoparticles and Calcium Hydroxide against Enterococcus faecalis Biofilm

2020 ◽  
Vol 46 (11) ◽  
pp. 1689-1694
Author(s):  
Hanan Balto ◽  
Sundus Bukhary ◽  
Omar Al-Omran ◽  
Anas BaHammam ◽  
Badr Al-Mutairi
Keyword(s):  
Silver Nanoparticles ◽  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Combined Effect

Comparison of the Antibacterial Efficacy of Silver Nanoparticles with Chlorhexidine Against Enterococcus faecalis Biofilm—An In Vitro Study

2017 ◽  
Vol 17 (7) ◽  
pp. 4613-4617 ◽  
Author(s):  
Venkateshbabu Nagendrababu ◽  
Gowri Meiyazhagan ◽  
Winfred Sofi Beaula ◽  
Rajeswari Kalaiselvam ◽  
Balakumar Subramanian ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Enterococcus Faecalis ◽  
In Vitro Study ◽  
Vitro Study ◽  
Antibacterial Efficacy
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