Antimicrobial and Antibiofilm Activity of Biosynthesized Silver Nanoparticles Against Beta-lactamase-Resistant Enterococcus faecalis

2022 ◽  
Author(s):  
Bikhal Fattah ◽  
Huner Arif ◽  
Haider Hamzah
Keyword(s):  
Silver Nanoparticles ◽  
Enterococcus Faecalis ◽  
Antibiofilm Activity ◽  
Beta Lactamase

Proteomic analysis of an Enterococcus faecalis mutant generated against the exposure to silver nanoparticles

2021 ◽  
Author(s):  
Marco Felipe Salas‐Orozco ◽  
Nereyda Niño‐Martínez ◽  
Gabriel‐Alejandro Martínez‐Castañón ◽  
Fernando Torres Méndez ◽  
Gabriela Margarita Montero Morán ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Enterococcus Faecalis ◽  
Proteomic Analysis

scholarly journals Biogenic Silver Nanoparticles Conjugated with Nisin: Improving the Antimicrobial and Antibiofilm Properties of Nanomaterials

Chemistry ◽  
2021 ◽  
Vol 3 (4) ◽  
pp. 1271-1285
Author(s):  
Patricia Zimet ◽  
Ruby Valadez ◽  
Sofía Raffaelli ◽  
María Belén Estevez ◽  
Helena Pardo ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antimicrobial Properties ◽  
Value Added ◽  
Green Technology ◽  
Natural Food ◽  
Antibiofilm Activity ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Biogenic Silver Nanoparticles

Microbial technology offers a green alternative for the synthesis of value-added nanomaterials. In particular, fungal compounds can improve silver nanoparticle production, stabilizing colloidal nanoparticles. Based on a previous study by our group, silver nanoparticles obtained using the extracellular cell-free extracts of Phanerochaete chrysosporium (PchNPs) have shown antimicrobial and antibiofilm activity against Gram-negative bacteria. Moreover, nisin—a bacteriocin widely used as a natural food preservative—has recently gained much attention due its antimicrobial action against Gram-positive bacteria in biomedical applications. Therefore, the aim of this work was to conjugate biogenic silver nanoparticles (PchNPs) with nisin to obtain nanoconjugates (PchNPs@nis) with enhanced antimicrobial properties. Characterization assays were conducted to determine physicochemical properties of PchNPs@nis, and also their antibacterial and antibiofilm activities were studied. The formation of PchNPs@nis was confirmed by UV-Vis, TEM, and Raman spectroscopy analysis. Different PchNPs@nis nanobioconjugates showed diameter values in the range of 60–130 nm by DLS and surface charge values between −20 and −13 mV. Nisin showed an excellent affinity to PchNPs, with binding efficiencies higher than 75%. Stable synthesized PchNPs@nis nanobioconjugates were not only able to inhibit biofilm formation by S. aureus, but also showed inhibition of the planktonic cell growth of Staphyloccocus aureus and Escherichia coli, broadening the spectrum of action of the unconjugated antimicrobials against Gram-positive and Gram-negative bacteria. In conclusion, these results show the promising application of PchNPs@nis, prepared via green technology, as potential antimicrobial nanomaterials.

scholarly journals Antibacterial efficacy of biosynthesized silver nanoparticles against Enterococcus faecalis Biofilm: An in vitro study

2018 ◽  
Vol 9 (2) ◽  
pp. 237 ◽  
Author(s):  
KiranRahul Halkai ◽  
JayashreeA Mudda ◽  
Vasundhara Shivanna ◽  
Vandana Rathod ◽  
Rahul Halkai
Keyword(s):  
Silver Nanoparticles ◽  
Enterococcus Faecalis ◽  
In Vitro Study ◽  
Vitro Study ◽  
Antibacterial Efficacy

scholarly journals Antibacterial Effects of Erbium Chromium Laser along with/without Silver Nanoparticles in Root Canals Infected by Enterococcus faecalis

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Seyedeh Sareh Hendi ◽  
Negin Amiri ◽  
Banafsheh Poormoradi ◽  
Mohammad Yousef Alikhani ◽  
Saeid Afshar ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Silver Nanoparticle ◽  
Colony Count ◽  
Antibacterial Effects ◽  
Bacterial Colony ◽  
Root Canals ◽  
Significant Difference ◽  
Root Canal System

This study investigates the antibacterial effects of erbium chromium laser at 2780 nm, silver nanoparticles, and erbium chromium along with silver nanoparticles on Enterococcus faecalis in comparison with sodium hypochlorite. In the present study, 90 extracted human single-rooted teeth were selected and standardized to a length of 15 mm. The canals were prepared by V-taper Gold rotary files and then incubated with E. faecalis for 21 days. The samples were divided into four experimental groups including hypochlorite sodium, silver nanoparticle, erbium chromium laser, and erbium chromium laser along with silver nanoparticle groups. Results showed that there was a significant reduction in colony count for all groups after interventions. Moreover, there was a significant reduction in the colony count for sodium hypochlorite group in comparison with another groups, and this group showed the highest reduction of colony count. There was a significant difference between silver nanoparticles and erbium chromium laser groups in colony count. According to the results, the silver nanoparticles offered strong antibacterial effects on E. faecalis and therefore can decrease bacterial colonies, while the use of the laser, despite the reduction of the bacterial colony, could not be sufficiently used for disinfection of root canal system.

scholarly journals The Antibiofilm Activity of Dual-Function Tail Tubular Protein B from KP32 Phage

2018 ◽  
Author(s):  
Ewa Brzozowska ◽  
Anna Pyra ◽  
Krzysztof Pawlik ◽  
Sabina Górska ◽  
Andrzej Gamian
Keyword(s):  
Klebsiella Pneumoniae ◽  
Enterococcus Faecalis ◽  
Biofilm Formation ◽  
Hydrolytic Activity ◽  
Bacterial Biofilm ◽  
Dual Function ◽  
Antibiofilm Activity ◽  
Synergistic Activity ◽  
Antibiotic Action ◽  
Phage Protein

Background: Dual function tail tubular proteins (TTP) belonging to the lytic bacteriophages are the interesting group of biologically active enzymes. Surprisingly, apart from their structural function, they are also polysaccharide hydrolyzes destroying bacterial extracellular components. One of the representatives of this group is TTPB from Klebsiella pneumoniae phage – KP32. TTPB hydrolyzes exopolysaccharide (EPS) of Klebsiella pneumoniae and Enterococcus faecalis strain. This depolymerizing feature was associated with the activity to prevent bacterial biofilm formation. TTPB can inhibit biofilm formation by K. pneumoniae, Enterobacter cloacae, Staphylococcus aureus, Enterococcus faecalis and Pseudomonas aeruginosa strains. Moreover, synergistic activity with antibiotic action has been observed, most likely due to depolymerases’ facilitation of contact of antibiotic with bacterial cells. Methods: TTPB was overexpressed in E coli system, purified and tested towards the bacterial strains using agar overlay method. The hydrolytic activity of TTPB was performed using EPSs of K. pneumoniae PCM2713 and E. cloacae ATCC 13047 as the substrates. Next, we determined the reducing sugar (RS) levels in the TTPB/EPS mixtures, regarding the RS amount obtained after acidic hydrolysis. The antibiofilm activity of TTPB has been set down on bacterial biofilm using a biochemical method. Finally, we have demonstrated the synergistic activity of TTPB with kanamycin. Results: For the first time, the hydrolytic activity of TTPB towards bacterial EPSs has been shown. TTPB releases about a half of the whole RS amount of EPSs belonging to K. pneumoniae PCM 2713 and E. cloacae ATCC 13047 strains. 1.12 µM of the phage protein reduces biofilm of both strains by over 60%. Destroying the bacterial biofilm the phage protein improves the antibiotic action increasing kanamycin effectiveness up to four times.

scholarly journals Effect of Silver Nanoparticles on Biofilm Formation and EPS Production of Multidrug-Resistant Klebsiella pneumoniae

2020 ◽  
Vol 2020 ◽  
pp. 1-9 ◽  
Author(s):  
Muhammad Hussnain Siddique ◽  
Bilal Aslam ◽  
Muhammad Imran ◽  
Asma Ashraf ◽  
Habibullah Nadeem ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Biofilm Formation ◽  
Extracellular Polymeric Substances ◽  
Microtiter Plate ◽  
Cellular Protein ◽  
Antibiofilm Activity ◽  
Biofilm Inhibition ◽  
Microtiter Plate Assay ◽  
Protein Leakage ◽  
Hela Cell Lines

Antibiotic resistance against present antibiotics is rising at an alarming rate with need for discovery of advanced methods to treat infections caused by resistant pathogens. Silver nanoparticles are known to exhibit satisfactory antibacterial and antibiofilm activity against different pathogens. In the present study, the AgNPs were synthesized chemically and characterized by UV-Visible spectroscopy, scanning electron microscopy, and X-ray diffraction. Antibacterial activity against MDR K. pneumoniae strains was evaluated by agar diffusion and broth microdilution assay. Cellular protein leakage was determined by the Bradford assay. The effect of AgNPs on production on extracellular polymeric substances was evaluated. Biofilm formation was assessed by tube method qualitatively and quantitatively by the microtiter plate assay. The cytotoxic potential of AgNPs on HeLa cell lines was also determined. AgNPs exhibited an MIC of 62.5 and 125 μg/ml, while their MBC is 250 and 500 μg/ml. The production of extracellular polymeric substance decreased after AgNP treatment while cellular protein leakage increased due to higher rates of cellular membrane disruption by AgNPs. The percentage biofilm inhibition was evaluated to be 64% for K. pneumoniae strain MF953600 and 86% for MF953599 at AgNP concentration of 100 μg/ml. AgNPs were evaluated to be minimally cytotoxic and safe at concentrations of 15-120 μg/ml. The data evaluated by this study provided evidence of AgNPs being safe antibacterial and antibiofilm compounds against MDR K. pneumoniae.

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