Screening of gamma‐aminobutyric acid‐producing lactic acid bacteria and the characteristic of glutamate decarboxylase from Levilactobacillus brevis F109‐MD3 isolated from kimchi

Glutamate decarboxylase (L-glutamate-1-carboxylase, GAD; EC 4.1.1.15) is a pyridoxal 5-phosphate-dependent enzyme, which catalyzes the irreversible α-decarboxylation of L-glutamic acid to γ-aminobutyric acid (GABA) and CO2. The enzyme is widely distributed in eukaryotes as well as prokaryotes, where it – together with its reaction product GABA - fulfils very different physiological functions. The occurrence of gad genes encoding GAD has been shown for many microorganisms, and GABA-producing lactic acid bacteria (LAB) have been a focus of research during recent years. A wide range of traditional foods produced by fermentation based on LAB offer the potential of providing new functional food products enriched with GABA that may offer certain health-benefits. Different GAD enzymes and genes from several strains of LAB have been isolated and characterized recently. GABA-producing LAB, biochemical properties of their GAD enzymes, and possible applications are reviewed here.

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Selection of Lactic Acid Bacteria with High Gamma-Aminobutyric Acid Productivity and Their Application to Fermented Chicken Sauce

Nippon Shokuhin Kagaku Kogaku Kaishi ◽

10.3136/nskkk.60.125 ◽

2013 ◽

Vol 60 (3) ◽

pp. 125-132

Author(s):

Gechuan Hou ◽

Hiroe Fujikawa ◽

Kensuke Arakawa ◽

Taku Miyamoto

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Aminobutyric Acid ◽

Gamma Aminobutyric Acid ◽

Acid Productivity ◽

High Gamma ◽

Selection Of

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Isolation and Characterization of Gamma-Aminobutyric Acid (GABA)-Producing Lactic Acid Bacteria from Kimchi

Current Topic in Lactic Acid Bacteria and Probiotics ◽

10.35732/ctlabp.2020.6.2.64 ◽

2020 ◽

Vol 6 (2) ◽

pp. 64-69

Author(s):

Eunyeong Hwang ◽

Jae-Yong Park

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Aminobutyric Acid ◽

Gamma Aminobutyric Acid ◽

Isolation And Characterization

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Glutamate Decarboxylase from Lactic Acid Bacteria—A Key Enzyme in GABA Synthesis

Microorganisms ◽

10.3390/microorganisms8121923 ◽

2020 ◽

Vol 8 (12) ◽

pp. 1923

Author(s):

Ida Bagus Agung Yogeswara ◽

Suppasil Maneerat ◽

Dietmar Haltrich

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Glutamate Decarboxylase ◽

Biochemical Properties ◽

Aminobutyric Acid ◽

Traditional Foods ◽

Wide Range ◽

Genes Encoding ◽

Key Enzyme ◽

Gaba Synthesis

Glutamate decarboxylase (l-glutamate-1-carboxylase, GAD; EC 4.1.1.15) is a pyridoxal-5’-phosphate-dependent enzyme that catalyzes the irreversible α-decarboxylation of l-glutamic acid to γ-aminobutyric acid (GABA) and CO2. The enzyme is widely distributed in eukaryotes as well as prokaryotes, where it—together with its reaction product GABA—fulfils very different physiological functions. The occurrence of gad genes encoding GAD has been shown for many microorganisms, and GABA-producing lactic acid bacteria (LAB) have been a focus of research during recent years. A wide range of traditional foods produced by fermentation based on LAB offer the potential of providing new functional food products enriched with GABA that may offer certain health-benefits. Different GAD enzymes and genes from several strains of LAB have been isolated and characterized recently. GABA-producing LAB, the biochemical properties of their GAD enzymes, and possible applications are reviewed here.

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