Differences in biofilm formation of Salmonella serovars on two surfaces under two temperature conditions

2021 ◽  
Author(s):  
Tomi Obe ◽  
Amber K. Richards ◽  
Nikki W. Shariat
Keyword(s):  
Biofilm Formation ◽  
Temperature Conditions ◽  
Salmonella Serovars ◽  
Two Temperature

Effect of bile on growth and biofilm formation of non-typhoidal salmonella serovars isolated from seafood and poultry

2020 ◽  
Vol 171 (5-6) ◽  
pp. 165-173 ◽  
Author(s):  
Akshatha Kotian ◽  
Vankadari Aditya ◽  
Kadeeja Jazeela ◽  
Iddya Karunasagar ◽  
Indrani Karunasagar ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Salmonella Serovars

scholarly journals Corn Grind Size and Broilers Reared Under Two Temperature Conditions

1995 ◽  
Vol 4 (4) ◽  
pp. 402-406 ◽  
Author(s):  
J.W. Deaton ◽  
B.D. Lott ◽  
S.L. Branton
Keyword(s):  
Temperature Conditions ◽  
Two Temperature

scholarly journals Fate and Biofilm Formation of Wild-Type and Pressure-Stressed Pathogens of Public Health Concern in Surface Water and on Abiotic Surfaces

2020 ◽  
Vol 8 (3) ◽  
pp. 408 ◽  
Author(s):  
Md Niamul Kabir ◽  
Sadiye Aras ◽  
Sabrina Wadood ◽  
Shahid Chowdhury ◽  
Aliyar Cyrus Fouladkhah
Keyword(s):  
Public Health ◽  
Surface Water ◽  
Biofilm Formation ◽  
Health Concern ◽  
Wild Type ◽  
Produce Safety ◽  
Abiotic Surfaces ◽  
Safety Rule ◽  
Public Health Challenge ◽  
Salmonella Serovars

Since the historic outbreak near Broad Street in London, which serves as cornerstone of modern epidemiology, infectious diseases spread in surface and sub-surface water has been a persisting public health challenge. The current study investigated persistence of wild-type and pressure-stressed Listeria monocytogenes, Escherichia coli O157:H7, and non-typhoidal Salmonella enterica serovars in surface water stored aerobically for up to 28 days at 5, 25, and 37 °C. Additionally, biofilm formation of wild-type and pressure-stressed non-typhoidal Salmonella serovars were monitored on surface of stainless steel and rubber coupons for 28 days at 25 and 37 °C. While L. monocytogenes exhibited a lower (p < 0.05) survival rate at 5 °C, relative to the two Gram-negative pathogens, at higher temperatures of 25 and 37 °C, all three pathogens exhibited similar (p ≥ 0.05) trends for survival in surface water. Both wild-type and pressure-stressed Salmonella serovars in the vast majority of tested times, temperatures, and surfaces exhibited comparable (p ≥ 0.05) persistence and biofilm formation capability. Our study thus indicates the occurrence of contamination could lead to prolonged survival of these microorganisms in low-nutrient environments and highlights the need for preventive measures such as those articulated under Produce Safety Rule of the U.S. Food Safety Modernization Act.

RECIPROCAL CROSS ANALYSIS OF GROWTH COMPONENT STAGES IN TOMATOES UNDER TWO TEMPERATURE REGIMES

1980 ◽  
Vol 60 (1) ◽  
pp. 163-166
Author(s):  
THOMAS SHIN-CHAI LI ◽  
C. A. HORNBY
Keyword(s):  
Reciprocal Cross ◽  
Reciprocal Hybrids ◽  
Temperature Conditions ◽  
Cytoplasmic Effects ◽  
Temperature Regimes ◽  
Cross Experiment ◽  
Selection For ◽  
Two Temperature

The nuclear and cytoplasmic effects on seven growth component stages in tomatoes were studied in a reciprocal cross experiment involving two parental cultivars, Bonny Best and Immur Prior Beta, and their reciprocal hybrids. These were grown under the two temperature regimes in greenhouses, 17 °C–21 °C and 10 °C–13 °C respectively. There was evidence that cytoplasmic effects were relatively important for some of the characteristics under stress temperature condition. It is suggested that the cytoplasmic effects and genic-cytoplasm interactions should be considered during selection for tomatoes under subnormal temperature conditions.

scholarly journals Clonal persistence of Salmonella enterica serovars Montevideo, Tennessee, and Infantis in feed factories

2016 ◽  
Vol 10 (06) ◽  
pp. 662-666 ◽  
Author(s):  
Bojana Prunić ◽  
Dubravka Milanov ◽  
Maja Velhner ◽  
Marko Pajić ◽  
Ljiljana Pavlović ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Salmonella Enterica ◽  
Biofilm Formation ◽  
Animal Feed ◽  
Molecular Techniques ◽  
Behavior Patterns ◽  
Test Results ◽  
Biotechnology Research ◽  
Salmonella Serovars ◽  
Feed Samples

Introduction: Novel molecular techniques applied in biotechnology research have provided sound evidence on clonal persistence of distinct serovars of Salmonella in feed factory environments, over long periods of time (months, even years), which can be responsible for repeated in-house contamination of final products. In this study, we examined the possibility of clonal persistence of isolates of three Salmonella serovars that have been repeatedly identified in animal feed samples from three feed factories throughout a two-year period. Methodology: The isolates Salmonella enterica serovars Tennessee (n = 7), Montevideo (n = 8), and Infantis (n = 4) were tested for genetic diversity using pulsed-field gel electrophoresis (PFGE) and multicellular behavior patterns by applying the Congo red agar test. Results: SpeI and XbaI macro-restriction profiles indicated that isolates S. Montevideo and S. Infantis were identical, whereas isolates of S. Tennessee demonstrated greater genetic diversity, although the genetic differences did not exceed 10%. All Salmonella serovars demonstrated the ability to produce predominant matrix compounds essential for biofilm formation, curli fimbriae and cellulose. Conclusions: The identification of identical clones of S. Montevideo and S. Infantis, as well as the minor genetic diversity of S. Tennessee, which have been repeatedly isolated from animal feed in three production plants throughout a two-year period, indirectly suggests the possibility of their persistence in feed factory environments. Their ability to express the key biofilm matrix components further supports this hypothesis.

scholarly journals Biofilm formation capacity of Salmonella serotypes at different temperature conditions

2018 ◽  
Vol 38 (1) ◽  
pp. 71-76 ◽  
Author(s):  
Karen A. Borges ◽  
Thales Q. Furian ◽  
Sara N. Souza ◽  
Rafaela Menezes ◽  
Eduardo C. Tondo ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Production Capacity ◽  
Microtiter Plate ◽  
Bacterial Cells ◽  
Bacterial Survival ◽  
Salmonella Spp ◽  
Microtiter Plate Assay ◽  
Temperature Conditions ◽  
Biofilm Production ◽  
Salmonella Serotypes

ABSTRACT: Salmonella spp. are one of the most important agents of foodborne disease in several countries, including Brazil. Poultry-derived products are the most common food products, including meat and eggs, involved in outbreaks of human salmonellosis. Salmonella has the capacity to form biofilms on both biotic and abiotic surfaces. The biofilm formation process depends on an interaction among bacterial cells, the attachment surface and environmental conditions. These structures favor bacterial survival in hostile environments, such as slaughterhouses and food processing plants. Biofilms are also a major problem for public health because breakage of these structures can cause the release of pathogenic microorganisms and, consequently, product contamination. The aim of this study was to determine the biofilm production capacity of Salmonella serotypes at four different temperatures of incubation. Salmonella strains belonging to 11 different serotypes, isolated from poultry or from food involved in salmonellosis outbreaks, were selected for this study. Biofilm formation was investigated under different temperature conditions (37°, 28°, 12° and 3°C) using a microtiter plate assay. The tested temperatures are important for the Salmonella life cycle and to the poultry-products process. A total of 92.2% of the analyzed strains were able to produce biofilm on at least one of the tested temperatures. In the testing, 71.6% of the strains produced biofilm at 37°C, 63% at 28°C, 52.3% at 12°C and 39.5% at 3°C, regardless of the serotype. The results indicate that there is a strong influence of temperature on biofilm production, especially for some serotypes, such as S. Enteritidis, S. Hadar and S. Heidelberg. The production of these structures is partially associated with serotype. There were also significant differences within strains of the same serotype, indicating that biofilm production capacity may be strain-dependent.

Bacterial biofilm behavior in water-supply lines of dental units

1992 ◽  
Vol 50 (1) ◽  
pp. 882-883
Author(s):  
B.D. Tall ◽  
K.S. George ◽  
R. T. Gray ◽  
H.N. Williams
Keyword(s):  
Water Supply ◽  
Medical Devices ◽  
Biofilm Formation ◽  
Bacterial Biofilm

Studies of bacterial behavior in many environments have shown that most organisms attach to surfaces, forming communities of microcolonies called biofilms. In contaminated medical devices, biofilms may serve both as reservoirs and as inocula for the initiation of infections. Recently, there has been much concern about the potential of dental units to transmit infections. Because the mechanisms of biofilm formation are ill-defined, we investigated the behavior and formation of a biofilm associated with tubing leading to the water syringe of a dental unit over a period of 1 month.

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