scholarly journals PD164: The Anti-inflammatory effect of a gut microbial metabolite (10-oxo-trans-11-octadecenoic acid) on macrophages stimulated with Porphyromonas gingivalis lipopolysaccharide

2018 ◽  
Vol 45 ◽  
pp. 100-101
Keyword(s):  
Porphyromonas Gingivalis ◽  
Octadecenoic Acid ◽  
Microbial Metabolite ◽  
Porphyromonas Gingivalis Lipopolysaccharide ◽  
Anti Inflammatory ◽  
Inflammatory Effect

scholarly journals Sitagliptin Attenuates Porphyromonas Gingivalis Virulence and Inflammatory Response in Macrophage on Titanium

2021 ◽  
Author(s):  
Weilong Tang ◽  
Minquan Du ◽  
Shuang Zhang ◽  
Han Jiang
Keyword(s):  
Inflammatory Response ◽  
Porphyromonas Gingivalis ◽  
Virulence Factors ◽  
Flow Cytometry Assay ◽  
Related Factors ◽  
Qrt Pcr ◽  
Inhibiting Effect ◽  
Porphyromonas Gingivalis Lipopolysaccharide ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Abstract BackgroundIn peri-implantitis, porphyromonas gingivalis and macrophage play central roles. The aim of this study was to detect the attenuating effect of an anti-diabetic drug sitagliptin on porphyromonas gingivalis virulence and inflammatory response in macrophage on titanium discs. Materials and methodsPorphyromonas gingivalis and macrophage were cultured on titanium discs. Antibacterial and antibiofilm activities of sitagliptin were assessed and the morphology of porphyromonas gingivalis were observed by SEM. Bacterial early adhesion, aggregation, hemagglutination, hemolysis and porphyromonas gingivalis virulence factors mRNA expression were assessed to preliminarily investigate the mechanisms of action. Flow cytometry assay, qRT-PCR and Western Blot were used to assess the anti-inflammatory effect of sitagliptin on porphyromonas gingivalis lipopolysaccharide-stimulated macrophage. ResultsThe present study demonstrated the inhibiting effect of sitagliptin on the growth, biofilm, phenotypic behavior and virulence factors of porphyromonas gingivalis and the protective effect on the porphyromonas gingivalis lipopolysaccharide-induced polarization in macrophage. And we also confirmed the anti-inflammatory effect of sitagliptin on the secretion of inflammation-related factors in macrophage by inhibiting the MAPK and AKT signaling pathways. ConclusionsSitagliptin possesses the attenuating effect on porphyromonas gingivalis virulence and inflammatory response in porphyromonas gingivalis lipopolysaccharide-stimulated macrophage on titanium.

scholarly journals Anti-Inflammatory Effect of Ecklonia cava Extract on Porphyromonas gingivalis Lipopolysaccharide-Stimulated Macrophages and a Periodontitis Rat Model

Nutrients ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 1143 ◽  
Author(s):  
Seonyoung Kim ◽  
Soo-Im Choi ◽  
Gun-Hee Kim ◽  
Jee-Young Imm
Keyword(s):  
Porphyromonas Gingivalis ◽  
Nuclear Factor Κb ◽  
Ecklonia Cava ◽  
Heme Oxygenase 1 ◽  
Therapeutic Effects ◽  
Related Factor ◽  
Nuclear Factor ◽  
Porphyromonas Gingivalis Lipopolysaccharide ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Ecklonia cava, an edible marine brown alga (Laminariaceae), is a rich source of phlorotannins. This study aimed to investigate the anti-inflammatory effect of Ecklonia cava ethanol extract (ECE, dieckol 10.6%, w/w) on Porphyromonas gingivalis lipopolysaccharide-stimulated inflammation in RAW 264.7 cells and in ligature-induced periodontitis in rats. The levels of nitric oxide (NO) and prostaglandin E2 were decreased by more than half on treatment with 100 μg/mL ECE. Downregulated tumor necrosis factor-α, interleukin (IL)-1β, and IL-6 gene expression confirmed the anti-inflammatory properties of ECE. ECE treatment upregulated heme oxygenase-1 (HO-1) expression by 6.3-fold and increased HO-1/nuclear factor erythroid 2-related factor 2 (Nrf-2) signaling decreased nuclear factor-κB (NF-κB) translocation. ECE administration (400 mg/kg) significantly reduced gingival index, restricted tooth mobility, and prevented alveolar bone loss (p < 0.05). These beneficial effects were due to decreased inflammatory cell infiltration, IL-1β production, and matrix metalloproteinase expression in gingival tissues. The ratio of receptor activator of nuclear factor-κB ligand (RANKL)/osteoprotegerin, a biomarker of periodontitis and osteolysis, was significantly decreased by ECE administration (p < 0.05). Thus, ECE has potential therapeutic effects for the alleviation of periodontal disease.

scholarly journals 5-Dodecanolide, a Compound Isolated from Pig Lard, Presents Powerful Anti-Inflammatory Properties

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7363
Author(s):  
Xavier Capó ◽  
Miquel Martorell ◽  
Josep A. Tur ◽  
Antoni Sureda ◽  
Antoni Pons
Keyword(s):  
Mononuclear Cells ◽  
Octadecenoic Acid ◽  
Resolvin D1 ◽  
Hydroalcoholic Extract ◽  
Inflammatory Agent ◽  
Pork Lard ◽  
Peripheral Mononuclear Cells ◽  
Anti Inflammatory ◽  
Main Components ◽  
Inflammatory Effect

Background: Pork lard (PL) is traditionally used as an anti-inflammatory agent. We propose to demonstrate the anti-inflammatory properties of PL, and elucidate which compounds could be responsible for the anti-inflammatory effects. Methods: The anti-inflammatory effects of PL were tested in a rat model of zymosan-induced hind paw inflammation. Further, the hydroalcoholic extract from PL was obtained, the composition analyzed, and the anti-inflammatory activity of the extracts and isolated components assayed using immune cells stimulated with lipopolysaccharide (LPS). Results: Applying the ointment on the inflamed rat feet reduced the foot diameter, foot weight, and activities of antioxidant enzymes and inflammatory markers of circulating neutrophils. The main components of the hydroalcoholic extract were 5-dodecanolide, oleamide, hexadecanoic acid, 9-octadecenoic acid, hexadecanamide, and resolvin D1. Conclusions: PL reduces the immune response in an animal model stimulated with zymosan. Hydroalcoholic PL extract and its components (5-Dodecanolide, Oleamide, and Resolvin D1) exerted an anti-inflammatory effect on LPS-stimulated neutrophils and peripheral mononuclear cells reducing the capability to produce TNFα, as well as the activities of antioxidant and pro-inflammatory enzymes. These effects are attributable to 5-dodecanolide, although the effects of this compound alone do not reach the magnitude of the anti-inflammatory effects observed by the complete hydroalcoholic extract.

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