scholarly journals 5-Dodecanolide, a Compound Isolated from Pig Lard, Presents Powerful Anti-Inflammatory Properties

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7363
Author(s):  
Xavier Capó ◽  
Miquel Martorell ◽  
Josep A. Tur ◽  
Antoni Sureda ◽  
Antoni Pons
Keyword(s):  
Mononuclear Cells ◽  
Octadecenoic Acid ◽  
Resolvin D1 ◽  
Hydroalcoholic Extract ◽  
Inflammatory Agent ◽  
Pork Lard ◽  
Peripheral Mononuclear Cells ◽  
Anti Inflammatory ◽  
Main Components ◽  
Inflammatory Effect

Background: Pork lard (PL) is traditionally used as an anti-inflammatory agent. We propose to demonstrate the anti-inflammatory properties of PL, and elucidate which compounds could be responsible for the anti-inflammatory effects. Methods: The anti-inflammatory effects of PL were tested in a rat model of zymosan-induced hind paw inflammation. Further, the hydroalcoholic extract from PL was obtained, the composition analyzed, and the anti-inflammatory activity of the extracts and isolated components assayed using immune cells stimulated with lipopolysaccharide (LPS). Results: Applying the ointment on the inflamed rat feet reduced the foot diameter, foot weight, and activities of antioxidant enzymes and inflammatory markers of circulating neutrophils. The main components of the hydroalcoholic extract were 5-dodecanolide, oleamide, hexadecanoic acid, 9-octadecenoic acid, hexadecanamide, and resolvin D1. Conclusions: PL reduces the immune response in an animal model stimulated with zymosan. Hydroalcoholic PL extract and its components (5-Dodecanolide, Oleamide, and Resolvin D1) exerted an anti-inflammatory effect on LPS-stimulated neutrophils and peripheral mononuclear cells reducing the capability to produce TNFα, as well as the activities of antioxidant and pro-inflammatory enzymes. These effects are attributable to 5-dodecanolide, although the effects of this compound alone do not reach the magnitude of the anti-inflammatory effects observed by the complete hydroalcoholic extract.

scholarly journals The Effect of Chronic Spice Consumption on Plasma Pro-Inflammatory Cytokine Levels and Monocyte Function in Adults with Overweight/Obesity

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 1530-1530
Author(s):  
Ester Oh ◽  
Kristina Petersen ◽  
Penny Kris-Etherton ◽  
Connie Rogers
Keyword(s):  
Inflammatory Cytokine ◽  
Mononuclear Cells ◽  
Transendothelial Migration ◽  
Test Period ◽  
Low Grade ◽  
Monocyte Subset ◽  
Cytokine Levels ◽  
Anti Inflammatory ◽  
Classical Monocytes ◽  
Inflammatory Effect

Abstract Objectives Obesity-induced, chronic, low-grade inflammation is involved in the pathogenesis of cardiovascular disease (CVD). Numerous spices have anti-inflammatory properties in animal models and humans. However, few studies have examined the anti-inflammatory effect of spices in the context of daily meal consumption, which is typically how spices are consumed. The objective of this study was to investigate the anti-inflammatory effect of chronic spice consumption in adults with overweight/obesity at risk for CVD. Methods Nonsmoking adults (30–75 years old) with overweight/obesity (BMI ≥ 25 and ≤ 35 kg/m2), elevated waist circumference (≥94 cm for men and ≥ 80 cm for women) and at least one other risk factor for CVD were recruited for a 3-period, crossover, randomized controlled-feeding study (n = 63). In random order, participants consumed an Average American Diet (AAD) for 4 weeks containing: 1) 0.6 g of spice blend per 2100 kcal, 2) 3.2 g of spice blend per 2100 kcal, or 3) 6.4 g of spice blend per 2100 kcal with a≥2-week washout period between each test period. The spice blend was comprised of 24 popular spices. Blood was collected at baseline and after each test period. Peripheral blood mononuclear cells were isolated, and the % of monocyte subsets (classical; CD14++CD16−, intermediate; CD14++CD16+, non-classical; CD14+CD16++) were quantified using flow cytometry. Plasma pro-inflammatory cytokine levels (TNF-α, IL-1β, IL-6, IL-8, MCP-1) were measured using ELISA. In a subset of participants (n = 6), transendothelial migratory function of each monocyte subset through MCP-1 stimulated human umbilical vein endothelial cells was evaluated. Results Plasma IL-6 was significantly reduced after consuming the AAD containing 3.2 g compared to 0.6 g of spice blend in men and postmenopausal women (P = 0.031). Transendothelial migration of classical monocytes was significantly reduced following consumption of the AAD containing 3.2 g and 6.4 g of the spice blend compared to 0.6 g of spice blend (P = 0.011). Conclusions Consumption of an AAD with spices for 4 weeks attenuated inflammatory outcomes including plasma IL-6 and transendothelial migration of classical monocytes in adults with overweight/obesity. Funding Sources McCormick Science Institute.

scholarly journals Rare Acetogenins with Anti-Inflammatory Effect from the Red Alga Laurencia obtusa

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 476
Author(s):  
Walied Alarif ◽  
Sultan Al-Lihaibi ◽  
Nahed Bawakid ◽  
Ahmed Abdel-Lateff ◽  
Hamdan Al-malky
Keyword(s):  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Red Alga ◽  
Peripheral Blood Mononuclear ◽  
Organic Extract ◽  
Chemical Structures ◽  
Data Analyses ◽  
Tnf Α ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Three new rare C12 acetogenins (enyne derivatives 1–3) were isolated from the organic extract obtained from the red alga Laurencia obtusa, collected from the Red Sea. The chemical structures of the isolated compounds were established by spectroscopical data analyses. Potent anti-inflammatory effect of the isolated metabolites was evidenced by inhibition of the release of inflammatory mediators (e.g., TNF-α, IL-1β and IL-6) by employing Human Peripheral Blood Mononuclear Cells (PBMC).

scholarly journals Curcumin Inhibits In Vitro SARS-CoV-2 Infection In Vero E6 Cells through Multiple Antiviral Mechanisms

Molecules ◽  
2021 ◽  
Vol 26 (22) ◽  
pp. 6900
Author(s):  
Damariz Marín-Palma ◽  
Jorge H. Tabares-Guevara ◽  
María I. Zapata-Cardona ◽  
Lizdany Flórez-Álvarez ◽  
Lina M. Yepes ◽  
...  
Keyword(s):  
Post Infection ◽  
Mononuclear Cells ◽  
Plaque Assay ◽  
Antiviral Effect ◽  
Peripheral Blood Mononuclear ◽  
Infection Treatment ◽  
Virucidal Effect ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Due to the scarcity of therapeutic approaches for COVID-19, we investigated the antiviral and anti-inflammatory properties of curcumin against SARS-CoV-2 using in vitro models. The cytotoxicity of curcumin was evaluated using MTT assay in Vero E6 cells. The antiviral activity of this compound against SARS-CoV-2 was evaluated using four treatment strategies (i. pre–post infection treatment, ii. co-treatment, iii. pre-infection, and iv. post-infection). The D614G strain and Delta variant of SARS-CoV-2 were used, and the viral titer was quantified by plaque assay. The anti-inflammatory effect was evaluated in peripheral blood mononuclear cells (PBMCs) using qPCR and ELISA. By pre–post infection treatment, Curcumin (10 µg/mL) exhibited antiviral effect of 99% and 99.8% against DG614 strain and Delta variant, respectively. Curcumin also inhibited D614G strain by pre-infection and post-infection treatment. In addition, curcumin showed a virucidal effect against D614G strain and Delta variant. Finally, the pro-inflammatory cytokines (IL-1β, IL-6, and IL-8) released by PBMCs triggered by SARS-CoV-2 were decreased after treatment with curcumin. Our results suggest that curcumin affects the SARS-CoV-2 replicative cycle and exhibits virucidal effect with a variant/strain independent antiviral effect and immune-modulatory properties. This is the first study that showed a combined (antiviral/anti-inflammatory) effect of curcumin during SARS-CoV-2 infection. However, additional studies are required to define its use as a treatment for the COVID-19.

scholarly journals Curcumin and Curcumol Inhibit NF-κB and TGF-β1/Smads Signaling Pathways in CSE-Treated RAW246.7 Cells

2019 ◽  
Vol 2019 ◽  
pp. 1-9
Author(s):  
Ning Li ◽  
Tian-Hao Liu ◽  
Jing-Ze Yu ◽  
Chen-Xi Li ◽  
Yang Liu ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Signaling Pathway ◽  
Treated Group ◽  
Macrophage Cells ◽  
Injury Model ◽  
Intracellular Ros ◽  
Anti Inflammatory ◽  
Main Components ◽  
Inflammatory Effect

E-Zhu (Curcuma zedoaria) is known as a classical traditional Chinese medicine and widely used in the treatment of cancers, cardiovascular disease, inflammation, and other diseases. Its main components include curcumol and curcumin, which have anti-inflammatory and antifibrosis effects. Here we established an in vitro inflammatory injury model by stimulating RAW246.7 cells with cigarette smoke extract (CSE) and detected the intervention effects of curcumin and curcumol on CSE-treated Raw246.7 macrophage cells to explore whether the two compounds inhibited the expression of inflammatory cytokines by inhibiting the NF-κB signaling pathway. We detected the antifibrosis effects of curcumin and curcumol via TGF-β1/Smads signaling pathways. The model of macrophage damage group was established by CSE stimulation. Curcumol and curcumin were administered to Raw246.7 macrophage cells. The efficacy of curcumol and curcumin was evaluated by comparing the activation of proinflammatory factors, profibrotic factors, and NF-κB and TGF-β1/Smads signaling pathway. In addition, CSE-treated group was employed to detect whether the efficacy of curcumol and curcumin was dependent on the NF-κB signaling via the pretreatment with the inhibitor of NF-κB. Our findings demonstrated that curcumol and curcumin could reduce the release of intracellular ROS from macrophages, inhibit the NF-κB signaling pathway, and downregulate the release of proinflammatory factor. Curcumol and curcumin inhibited the TGF-β1/Smads signaling pathway and downregulated the release of fibrotic factors. Curcumin showed no anti-inflammatory effect in CSE-treated cells after the inhibition of NF-κB. Curcumol and curcumin showed an anti-inflammatory effect by inhibiting the NF-κB signaling pathway.

scholarly journals The Anti-Inflammatory Effect of Feiyangchangweiyan Capsule and Its Main Components on Pelvic Inflammatory Disease in Rats via the Regulation of the NF-κB and BAX/BCL-2 Pathway

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Yao Li ◽  
Qian Yang ◽  
Zhi-hui Shi ◽  
Min Zhou ◽  
Li Yan ◽  
...  
Keyword(s):  
Pelvic Inflammatory Disease ◽  
Inflammatory Disease ◽  
Pathogenic Bacteria ◽  
Inflammatory Cells ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Pathogen ◽  
Anti Inflammatory ◽  
Main Components ◽  
Female Specific ◽  
Inflammatory Effect

Although gastroenteritis and pelvic inflammatory disease (PID) occur in the gastrointestinal tract and pelvis, respectively, they display similar pathogeneses. The incidence of inflammation in these conditions is usually associated with dysbacteriosis, and, at times, they are caused by the same pathogenic bacteria, Escherichia coli and Streptococcus aureus. Feiyangchangweiyan capsule (FYC) is a traditional Chinese patent medicine that is widely used to treat bacterial dysentery and acute and chronic gastroenteritis. However, whether it has an effect on PID is unclear. The aim of this study was to investigate the anti-inflammatory effect of FYC and its main components, gallic acid (GA), ellagic acid (EA), and syringin (SY), on a pathogen-induced PID model and illustrate their potential mechanism of action. Female specific pathogen-free SD rats (n = 1110) were randomly divided into control, PID, FYC, GA, EA, SY, GA + EA, GA + SY, EA + SY, GA + EA + SY, and Fuke Qianjin capsule (FKC) positive groups. Histological examination and enzyme-linked immunosorbent assay (ELISA) were carried out as well as western blot analysis to detect the expression of NF-κB, BAX, BCL-2, and JNK. In this study, FYC and its main components dramatically suppressed the infiltration of inflammatory cells, reduced the production of IL-1β, TNF-α, and MCP-1, and elevated the IL-10 level to varying degrees. We also found that FYC and its main components inhibited the expression of BAX induced by infection and increased the expression of Bcl-2. FYC, GA, EA, and SY could also block the activation of the NF-κB pathway. Finally, we found that the phosphorylation of JNK could be decreased by FYC, GA, and SY. FYC and its main components exhibit anti-inflammatory effect on a pathogen-induced PID model by regulating the NF-κB and apoptosis signaling pathways.

Anti-inflammatory effect of curcumin involves downregulation of MMP-9 in blood mononuclear cells

2007 ◽  
Vol 7 (13) ◽  
pp. 1659-1667 ◽  
Author(s):  
K. Saja ◽  
Mani Shankar Babu ◽  
D. Karunagaran ◽  
P.R. Sudhakaran
Keyword(s):  
Mononuclear Cells ◽  
Blood Mononuclear Cells ◽  
Anti Inflammatory ◽  
Inflammatory Effect

scholarly journals Mechanism of the Anti-inflammatory Effect of Curcumin: PPAR-γActivation

PPAR Research ◽  
2007 ◽  
Vol 2007 ◽  
pp. 1-5 ◽  
Author(s):  
Asha Jacob ◽  
Rongqian Wu ◽  
Mian Zhou ◽  
Ping Wang
Keyword(s):  
Clinical Studies ◽  
Peroxisome Proliferator ◽  
Peroxisome Proliferator Activated Receptor ◽  
Commercial Grade ◽  
Inflammatory Agent ◽  
Anticancer Effects ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Curcumin, the phytochemical component in turmeric, is used as a dietary spice and a topical ointment for the treatment of inflammation in India for centuries. Curcumin (diferuloylmethane) is relatively insoluble in water, but dissolves in acetone, dimethylsulphoxide, and ethanol. Commercial grade curcumin contains 10–20% curcuminoids, desmethoxycurcumin, and bisdesmethoxycurcumin and they are as effective as pure curcumin. Based on a number of clinical studies in carcinogenesis, a daily oral dose of 3.6 g curcumin has been efficacious for colorectal cancer and advocates its advancement into Phase II clinical studies. In addition to the anticancer effects, curcumin has been effective against a variety of disease conditions in both in vitro and in vivo preclinical studies. The present review highlights the importance of curcumin as an anti-inflammatory agent and suggests that the beneficial effect of curcumin is mediated by the upregulation of peroxisome proliferator-activated receptor-γ(PPAR-γ) activation.

scholarly journals Anti-inflammatory effect of Schinus terebinthifolius Raddi hydroalcoholic extract on neutrophil migration in zymosan-induced arthritis

2015 ◽  
Vol 175 ◽  
pp. 490-498 ◽  
Author(s):  
Elaine Cruz Rosas ◽  
Luana Barbosa Correa ◽  
Tatiana de Almeida Pádua ◽  
Thadeu Estevam Moreira Maramaldo Costa ◽  
José Luiz Mazzei ◽  
...  
Keyword(s):  
Neutrophil Migration ◽  
Schinus Terebinthifolius ◽  
Hydroalcoholic Extract ◽  
Anti Inflammatory ◽  
Inflammatory Effect

scholarly journals Anti-inflammatory effect of hydroalcoholic extract of hibiscus rosa on acute and chronic inflammation

2016 ◽  
Vol 4 (2) ◽  
pp. 123
Author(s):  
Pankaj Verma ◽  
Swati Sharma ◽  
Vikas Kumar ◽  
Hema Chaudhary
Keyword(s):  
Chronic Inflammation ◽  
Soxhlet Extraction ◽  
Treated Group ◽  
Chronic Inflammatory Disease ◽  
Control Group ◽  
Hydroalcoholic Extract ◽  
Anti Inflammatory ◽  
Acute And Chronic Inflammation ◽  
Different Doses ◽  
Inflammatory Effect

Background: The present study was carried out to explore the efficiency of Indian herbal source from Hibiscus rosa against a chronic inflammatory disease. Hibiscus rosa belongs to Malvaceae, acts by suppression of inflammation mediators.Methods: Hydroalcoholic extract form Hibiscus rosa is prepared through soxhlet extraction and Diclofenac is used as the standard. Carrageenan and formaldehyde are administered to induce acute and chronic inflammation. Animals are divided into 6 groups with 6 animals each including Normal group, inflammatory control group, Diclofenac treated group and Hibiscus rosa treated group at different doses of 250 mg/kg, 500 mg/kg and 1000 mg/kg.Results: Different concentrations of Hibiscus rosa treated groups i.e. 250 mg/kg (p<0.05), 500 mg/kg (p<0.05) and 1000 mg/kg (p<0.01) showed significant reduction in Paw edema as compared to controls. Significant reduction in Body weight was also observed in Hibiscus rosa treated groups. Hematological profiles of Hibiscus rosa treated group are satisfying and significant.Conclusion: Results showed significant anti-inflammatory effect of Hydroalcoholic extract of Hibiscus rosa and justifying its therapeutic role in inflammatory condition..

scholarly journals Soft Coral Dendronephthya puetteri Extract Ameliorates Inflammations by Suppressing Inflammatory Mediators and Oxidative Stress in LPS-Stimulated Zebrafish

2018 ◽  
Vol 19 (9) ◽  
pp. 2695 ◽  
Author(s):  
Eun-A Kim ◽  
Yuling Ding ◽  
Hye-Won Yang ◽  
Soo-Jin Heo ◽  
Seung-Hong Lee
Keyword(s):  
Cell Death ◽  
Soft Coral ◽  
In Vivo Model ◽  
Dependent Manner ◽  
Soft Corals ◽  
Zebrafish Model ◽  
Inflammatory Agent ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Marine-derived extract and/or bioactive compounds have attracted increasing demand due to their unique and potential uses as cures for various inflammation-based diseases. Several studies revealed anti-inflammatory candidates found in soft corals. However, the effects of soft corals on inflammation in an in vivo model remain to be determined. Therefore, the extract of soft coral Dendronephthya puetteri (DPE) was investigated for an in vivo anti-inflammatory effect in a lipopolysaccharide (LPS)-stimulated zebrafish model to determine its potential use as a natural anti-inflammatory agent. We also investigated whether DPE has toxic effects in a zebrafish model. No significant changes were observed in terms of survival, heart beat rate, or developmental abnormalities in the zebrafish embryos exposed to a concentration below 100 µg/mL of DPE. Treating the zebrafish model with LPS-treatment significantly increased the ROS, NO generation, and cell death. However, DPE inhibited this LPS-stimulated ROS, NO generation, and cell death in a dose-dependent manner. In addition, DPE significantly reduced the mRNA expression of both iNOS and COX-2 and markedly suppressed the expression levels of the proinflammatory cytokines, TNF-α and IL-6, in an LPS-stimulated zebrafish model. These findings demonstrate that DPE has profound anti-inflammatory effect in vivo, suggesting that DPE might be a strong natural anti-inflammatory agent.

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