Inhibition kinetics of oligochitosan as an uncompetitive inhibitor on fuji apple polyphenol oxidase

2018 ◽  
Vol 42 (5) ◽  
pp. e12585
Author(s):  
Shuwei Guo ◽  
Li Zhang ◽  
Lin Zhang ◽  
Mengni Zhao ◽  
Xianghong Meng
Keyword(s):  
Polyphenol Oxidase ◽  
Inhibition Kinetics ◽  
Fuji Apple ◽  
Kinetics Of ◽  
Uncompetitive Inhibitor

Inhibition kinetics of polyphenol oxidase by glutamic acid

2006 ◽  
Vol 225 (1) ◽  
pp. 67-73 ◽  
Author(s):  
Serap Doğan ◽  
Pınar Turan ◽  
Mehmet Doğan ◽  
Mahir Alkan ◽  
Oktay Arslan
Keyword(s):  
Glutamic Acid ◽  
Polyphenol Oxidase ◽  
Inhibition Kinetics ◽  
Kinetics Of

Purification of Polyphenol Oxidase from Potato and Investigation of the Inhibitory Effects of Phenolic Acids on Enzyme Activity

2020 ◽  
Vol 27 (3) ◽  
pp. 187-192 ◽  
Author(s):  
Songül Bayrak ◽  
Cansu Öztürk ◽  
Yeliz Demir ◽  
Zuhal Alım ◽  
Ömer İrfan Küfrevioglu
Keyword(s):  
Polyphenol Oxidase ◽  
Phenolic Acids ◽  
Competitive Inhibition ◽  
Specific Activity ◽  
Inhibition Kinetics ◽  
Inhibitory Effects ◽  
Inhibition Effect ◽  
Ic50 Values ◽  
Sepharose 4B ◽  
Kinetics Of

Background: Polyphenol Oxidase (PPO) belongs to the oxidoreductase enzyme family. Methods: Here, PPO was purified from potato using Sepharose 4B-L-tyrosine-p-aminobenzoic acid affinity chromatography. It determined the interactions between some phenolic acids and the enzyme. Results: The enzyme was obtained with a specific activity of 15333.33 EU/mg protein and 7.87- fold purification. It was found that phenolic acids exhibited inhibitory properties for PPO. The IC50 values of the phenolic acids were found in the range of 0.36-2.12 mM, and their Ki values were found in the range of 0.28± 0.07-1.72±0.32 mM. It was determined that all studied compounds displayed a competitive inhibition effect. Among these compounds, 3-hydroxybenzoic acid was found to be the most effective PPO inhibitor (Ki: 0.28±0.07 mM). Conclusion: Investigating the inhibition kinetics of the enzyme will simplify the testing of PPO inhibitor candidates.

scholarly journals Inhibition Kinetics of Agaricus bisporus (J.E. Lange) Imbach Polyphenol Oxidase~!2009-08-11~!2009-11-20~!2010-03-12~!

2010 ◽  
Vol 3 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Hicham Gouzi ◽  
Thibaud Coradin ◽  
Estrella Nunez Delicado ◽  
M. Umit Unal ◽  
Abdelhafid Benmansour
Keyword(s):  
Polyphenol Oxidase ◽  
Agaricus Bisporus ◽  
Inhibition Kinetics ◽  
Kinetics Of
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