Kolaviron pre‐treatment suppresses 7, 12 dimethylbenzanthracene‐induced alterations in estrogen receptor‐α, CYP 1A1, oxidative stress and inflammation in female Wistar rats

2021 ◽  
Author(s):  
O. C. Attah ◽  
I. A. Umar ◽  
D. A. Ameh ◽  
G. E. Forcados ◽  
A. Muhammad ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Estrogen Receptor ◽  
Wistar Rats ◽  
Estrogen Receptor Α ◽  
Female Wistar Rats ◽  
Pre Treatment

Kolaviron Ameliorates 7, 12-Dimethylbenzanthracene - Induced Mammary Damage in Female Wistar Rats

2021 ◽  
Vol 21 ◽  
Author(s):  
Rabiatu B. Suleiman ◽  
Aliyu Muhammad ◽  
Ismaila A. Umara ◽  
Mohammed A. Ibrahima ◽  
Ochuko L. Erukainure ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Sialic Acid ◽  
Wistar Rats ◽  
Mrna Level ◽  
Estrogen Receptor Α ◽  
Tumor Formation ◽  
Mammary Tissue ◽  
Standard Drug ◽  
Female Wistar Rats ◽  
Α Level

Background: Kolaviron (KV) is a flavonoid rich portion obtained from Garcinia kola seeds with a number of reported pharmacological effects. However, its ameliorative effects on 7,12-Dimethylbenzanthracene (DMBA)-induced mammary damage has not been fully investigated, despite the reported use of the seeds in the treatment of inflammatory related disorders. Objective: To evaluate the ameliorative effects of KV on DMBA-induced mammary damage in female Wistar rats. Methods: Forty-nine (49) female Wistar rats were randomly assigned into seven groups of seven rats each. DMBA was administered orally to rats in five of the groups as a single dose of 80 mg/kg body wt while the remaining two groups received the vehicle. The rats were palpated weekly for 3 months to monitor tumor formation. After 3 months of DMBA administration, 1 ml of blood was collected to assay for estrogen receptor- α (ER-α) level. Thereafter, the vehicle (dimethyl sulfoxide) was daily administered to the negative control and positive control groups for the 14 days duration of the experiment while three groups were each given a daily oral dose of 50, 100 and 200 mg/kg body wt of KV for the duration of the experiment. The last DMBA-induced group received 10 mg/kg body wt of the standard drug tamoxifen twice in a week and the remaining DMBA-free group received 200 mg/kg body wt KV. Subsequently, the animals were humanly sacrificed and ER-α, sialic acids, sialidase, sialyltransferase levels were assay for in blood and mammary tissues followed by histopathological examinations. Results: Significantly higher levels of estrogen receptor-α (ER-α), formation of lobular neoplastic cells, epithelial hyperplasia, lymphocyte infiltration and increased sialylation were detected in DMBA-induced rats. Treatment with KV at 50, 100 and 200 mg/kg body weight resulted in a significant (p<0.05) decrease in ER-α level, significantly (p<0.05) lower free serum sialic acid (21.1%), total sialic acid level of the mammary tissue (21.57%), sialyltransferase activity (30.83%) as well as mRNA level of the sialyltransferase gene (ST3Gal1) were observed after KV interventions. Conclusion: The findings suggest that KV could be further explored in targeting DMBA-induced mammary damage implicated in mammary carcinogenesis.

Zingerone ameliorates cisplatin‐induced ovarian and uterine toxicity via suppression of sex hormone imbalances, oxidative stress, inflammation and apoptosis in female wistar rats

2018 ◽  
Vol 102 ◽  
pp. 517-530 ◽  
Author(s):  
Erdal kaygusuzoglu ◽  
Cuneyt Caglayan ◽  
Fatih Mehmet Kandemir ◽  
Serkan Yıldırım ◽  
Sefa Kucukler ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Wistar Rats ◽  
Sex Hormone ◽  
Female Wistar Rats

scholarly journals Effect of sodium (S)-2-hydroxyglutarate in male, and succinic acid in female Wistar rats against renal ischemia-reperfusion injury, suggesting a role of the HIF-1 pathway

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9438
Author(s):  
Eduardo Cienfuegos-Pecina ◽  
Tannya R. Ibarra-Rivera ◽  
Alma L. Saucedo ◽  
Luis A. Ramírez-Martínez ◽  
Deanna Esquivel-Figueroa ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Succinic Acid ◽  
Wistar Rats ◽  
Ischemia Reperfusion ◽  
The Other ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Female Wistar Rats ◽  
Dose Dependent ◽  
Nephroprotective Effect

Background Ischemia–reperfusion (IR) injury is the main cause of delayed graft function in solid organ transplantation. Hypoxia-inducible factors (HIFs) control the expression of genes related to preconditioning against IR injury. During normoxia, HIF-α subunits are marked for degradation by the egg-laying defective nine homolog (EGLN) family of prolyl-4-hydroxylases. The inhibition of EGLN stabilizes HIFs and protects against IR injury. The aim of this study was to determine whether the EGLN inhibitors sodium (S)-2-hydroxyglutarate [(S)-2HG] and succinic acid (SA) have a nephroprotective effect against renal IR injury in Wistar rats. Methods (S)-2HG was synthesized in a 22.96% yield from commercially available L-glutamic acid in a two-step methodology (diazotization/alkaline hydrolysis), and its structure was confirmed by nuclear magnetic resonance and polarimetry. SA was acquired commercially. (S)-2HG and SA were independently evaluated in male and female Wistar rats respectively after renal IR injury. Rats were divided into the following groups: sham (SH), nontoxicity [(S)-2HG: 12.5 or 25 mg/kg; SA: 12.5, 25, or 50 mg/kg], IR, and compound+IR [(S)-2HG: 12.5 or 25 mg/kg; SA: 12.5, 25, or 50 mg/kg]; independent SH and IR groups were used for each assessed compound. Markers of kidney injury (BUN, creatinine, glucose, and uric acid) and liver function (ALT, AST, ALP, LDH, serum proteins, and albumin), proinflammatory cytokines (IL-1β, IL-6, and TNF-α), oxidative stress biomarkers (malondialdehyde and superoxide dismutase), and histological parameters (tubular necrosis, acidophilic casts, and vascular congestion) were assessed. Tissue HIF-1α was measured by ELISA and Western blot, and the expression of Hmox1 was assessed by RT-qPCR. Results (S)-2HG had a dose-dependent nephroprotective effect, as evidenced by a significant reduction in the changes in the BUN, creatinine, ALP, AST, and LDH levels compared with the IR group. Tissue HIF-1α was only increased in the IR group compared to SH; however, (S)-2HG caused a significant increase in the expression of Hmox1, suggesting an early accumulation of HIF-1α in the (S)-2HG-treated groups. There were no significant effects on the other biomarkers. SA did not show a nephroprotective effect; the only changes were a decrease in creatinine level at 12.5 mg/kg and increased IR injury at 50 mg/kg. There were no effects on the other biochemical, proinflammatory, or oxidative stress biomarkers. Conclusion None of the compounds were hepatotoxic at the tested doses. (S)-2HG showed a dose-dependent nephroprotective effect at the evaluated doses, which involved an increase in the expression of Hmox1, suggesting stabilization of HIF-1α. SA did not show a nephroprotective effect but tended to increase IR injury when given at high doses.

Cyantraniliprole impairs reproductive parameters by inducing oxidative stress in adult female wistar rats

2021 ◽  
Author(s):  
Suellen Ribeiro da Silva Scarton ◽  
Felipe Tsuzuki ◽  
Marina Trevizan Guerra ◽  
Dayane Priscila dos Santos ◽  
Aldair Casagrande dos Santos ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Adult Female ◽  
Wistar Rats ◽  
Reproductive Parameters ◽  
Female Wistar Rats

Effects of chia (Salvia hispanica L.) on oxidative stress and inflammation in ovariectomized adult female Wistar rats

2019 ◽  
Vol 10 (7) ◽  
pp. 4036-4045 ◽  
Author(s):  
Bárbara Pereira da Silva ◽  
Renata Celi Lopes Toledo ◽  
Marcella Duarte Villas Mishima ◽  
Maria Eliza de Castro Moreira ◽  
Christiane Mileib Vasconcelos ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Adult Female ◽  
High Fat Diet ◽  
Wistar Rats ◽  
Lipid Profiles ◽  
Ovariectomized Rats ◽  
High Fat ◽  
Salvia Hispanica ◽  
Female Wistar Rats ◽  
Salvia Hispanica L

The study investigated the influence of chia consumption on inflammation, oxidative stress, and lipid profiles in female ovariectomized rats fed a high-fat diet.

scholarly journals Peroxiredoxin-1 protects estrogen receptor α from oxidative stress-induced suppression and is a protein biomarker of favorable prognosis in breast cancer

2014 ◽  
Vol 16 (4) ◽  
Author(s):  
Patrick C O’Leary ◽  
Marta Terrile ◽  
Malgorzata Bajor ◽  
Pawel Gaj ◽  
Bryan T Hennessy ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Oxidative Stress ◽  
Estrogen Receptor ◽  
Estrogen Receptor Α ◽  
Protein Biomarker ◽  
Peroxiredoxin 1 ◽  
Favorable Prognosis

scholarly journals Comparisons of Curative Effects of Chlorophyll fromSauropus androgynus(L) Merr Leaf Extract and Cu-Chlorophyllin on Sodium Nitrate-Induced Oxidative Stress in Rats

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Suparmi Suparmi ◽  
Minidian Fasitasari ◽  
Martanto Martosupono ◽  
Jubhar Christian Mangimbulude
Keyword(s):  
Oxidative Stress ◽  
Sodium Nitrate ◽  
Wistar Rats ◽  
Histological Evaluation ◽  
Female Wistar Rats ◽  
Male Wistar Rats ◽  
Liver And Kidney ◽  
Serum Biochemical ◽  
Hb Concentration ◽  
Rbc Count

Sodium nitrate (NaNO2) widely used as food additive for coloring and preserving meat has been reported to induce oxidative stress and cause histopathologic changes, nitrosative tissue damage, and lipid peroxidation in liver and kidney. Therefore, the present study compared the curative effect of chlorophyll fromSauropus androgynus(L) Merr and Cu-chlorophyllin as antioxidant in NaNO2-induced female Wistar rats based on haematological, serum biochemical, and histological evaluation. Thirty male Wistar rats were randomly assigned into six groups of five rats each. NaNO2were given at a subacute dose of 50 mg/kg bw intraperitoneally for 10 days. Chlorophyll fromS. androgynusand Cu-chlorophyllin from K-Liquid™ were given in the following 14 days at the two doses: 0,016 mg/mL and 0.008 mg/mL. NaNO2exposure resulted in significant reductions (p<0.05) in values of packed cell volume (PCV), haemoglobin (Hb) concentration and red blood cell (RBC) count, transferrin, and ferritin and elevation in malondialdehyde (MDA) level and schistocytes percentage with insignificant reductions in serum albumin and transferrin levels. Histology of kidney and liver were changed insignificantly (p>0.05) to normal values. Chlorophyll fromS. androgynusand Cu-chlorophyllin possess antioxidant potentials to protect against toxicities induced by sodium nitrate.

Calycosin inhibits oxidative stress-induced cardiomyocyte apoptosis via activating estrogen receptor-α/β

2016 ◽  
Vol 26 (1) ◽  
pp. 181-185 ◽  
Author(s):  
Bin Liu ◽  
Jingzhi Zhang ◽  
Weihua Liu ◽  
Ningning Liu ◽  
Xiuqiong Fu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Estrogen Receptor ◽  
Estrogen Receptor Α ◽  
Cardiomyocyte Apoptosis

scholarly journals Tributyltin Exposure Is Associated With Recognition Memory Impairments, Alterations in Estrogen Receptor α Protein Levels, and Oxidative Stress in the Brain of Female Mice

2021 ◽  
Vol 3 ◽  
Author(s):  
Igor Ferraz da Silva ◽  
Eduardo Merlo ◽  
Charles S. Costa ◽  
Jones B. Graceli ◽  
Lívia C. M. Rodrigues
Keyword(s):  
Oxidative Stress ◽  
Estrogen Receptor ◽  
Recognition Memory ◽  
Protein Expression ◽  
Estrogen Receptor Α ◽  
Behavioral Alterations ◽  
Brain Areas ◽  
Female Mice ◽  
Toxicological Effects ◽  
And Oxidative Stress

Tributyltin (TBT) is a persistent organometallic pollutant widely used in several agricultural and industrial processes. TBT exposure is associated with various metabolic, reproductive, immune, and cardiovascular abnormalities. However, few studies have evaluated the effects of TBT on behavior. In the present study, we aimed to investigate whether TBT exposure results in oxidative, neuroendocrine, and behavioral alterations. TBT was administered to adult female mice (250, 500, or 750 ng/kg/day or veh for 14 days), and their recognition memory was assessed. We have also evaluated estrogen receptor (ER)α protein expression and oxidative stress (OS) in brain areas related to memory, as well as the correlation between them. A reduction in short- and long-term recognition memory (STM and LTM) performance, as well as in total exploration time was observed in TBT mice. Reduced ERα protein expression was observed in the prefrontal cortex (PFC) and hippocampus of TBT mice, while an increase in TBARS concentration was observed in the PFC of treated animals. Collectively, these data suggest that TBT exposure impairs recognition memory in female mice as a result of, at least in part, its toxicological effects on ERα expression and OS in specific brain areas related to memory.

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