Comparisons of Curative Effects of Chlorophyll fromSauropus androgynus(L) Merr Leaf Extract and Cu-Chlorophyllin on Sodium Nitrate-Induced Oxidative Stress in Rats

Sodium nitrate (NaNO2) widely used as food additive for coloring and preserving meat has been reported to induce oxidative stress and cause histopathologic changes, nitrosative tissue damage, and lipid peroxidation in liver and kidney. Therefore, the present study compared the curative effect of chlorophyll fromSauropus androgynus(L) Merr and Cu-chlorophyllin as antioxidant in NaNO2-induced female Wistar rats based on haematological, serum biochemical, and histological evaluation. Thirty male Wistar rats were randomly assigned into six groups of five rats each. NaNO2were given at a subacute dose of 50 mg/kg bw intraperitoneally for 10 days. Chlorophyll fromS. androgynusand Cu-chlorophyllin from K-Liquid™ were given in the following 14 days at the two doses: 0,016 mg/mL and 0.008 mg/mL. NaNO2exposure resulted in significant reductions (p<0.05) in values of packed cell volume (PCV), haemoglobin (Hb) concentration and red blood cell (RBC) count, transferrin, and ferritin and elevation in malondialdehyde (MDA) level and schistocytes percentage with insignificant reductions in serum albumin and transferrin levels. Histology of kidney and liver were changed insignificantly (p>0.05) to normal values. Chlorophyll fromS. androgynusand Cu-chlorophyllin possess antioxidant potentials to protect against toxicities induced by sodium nitrate.

Download Full-text

Comparison of ameliorative effects of Taraxacum syriacum and N-acetylcysteine against acetaminophen-induced oxidative stress in rat liver and kidney

The Journal of Biochemistry ◽

10.1093/jb/mvaa107 ◽

2020 ◽

Author(s):

Reza Eshrati ◽

Mahvash Jafari ◽

Saeed Gudarzi ◽

Afshen Nazari ◽

Esmaeil Samizadeh ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Ethanol Extract ◽

Control Group ◽

Standard Drug ◽

Ameliorative Effect ◽

Male Wistar Rats ◽

Liver And Kidney ◽

Serum Biochemical

Abstract Taraxacum syriacum (TS) with natural antioxidant and pharmacological activities may be considered for treatment of oxidative stress induced by acetaminophen (APAP). The aim of this study was to evaluate the ameliorative effects of the ethanol extract of TS root against hepatorenal toxicity induced by APAP in comparison to N-acetylcysteine (NAC) as a standard drug. Thirty male Wistar rats were randomly divided into five groups. Control group; APAP (1 g/kg) group; APAP–NAC (160 mg/kg) group and APAP-TS100 and APAP-TS200 groups: APAP plus 100 and 200 mg/kg of TS extract, respectively. After 7 days treatment, serum and liver and kidney tissues were prepared and evaluated. TS extract ameliorated the increased lipid peroxidation level and decreased antioxidant enzymes activities and glutathione level in liver and kidney of APAP-treated rats. Moreover, treatment with the TS extract caused significant reduction in the histopathological damages and high levels of serum biochemical markers of hepatic and renal functions after APAP treatment. This study suggests that the extract of TS roots has dose-dependent ameliorative effect against APAP-induced oxidative damage in liver and kidney due to its free radical scavenging and antioxidant properties. The overall efficacy of the extract at 200 mg/kg dose is comparable with NAC.

Download Full-text

Amla (Emblica officinalis) improves hepatic and renal oxidative stress and the inflammatory response in hypothyroid female wistar rats fed with a high-fat diet

Journal of Basic and Clinical Physiology and Pharmacology ◽

10.1515/jbcpp-2017-0116 ◽

2018 ◽

Vol 29 (2) ◽

pp. 175-184 ◽

Cited By ~ 5

Author(s):

P. Rajaa Muthu ◽

Zachariah Bobby ◽

P. Sankar ◽

V. Vickneshwaran ◽

Sajini Elizabeth Jacob

Keyword(s):

Oxidative Stress ◽

Inflammatory Response ◽

High Fat Diet ◽

Wistar Rats ◽

Molecular Techniques ◽

Emblica Officinalis ◽

High Fat ◽

Tnf Α ◽

Female Wistar Rats ◽

Liver And Kidney

AbstractBackground:We investigated the protective effects of amla (Emblica officinalis) on the pathogenesis of oxidative stress (OS) and inflammatory response in hypothyroid rats fed with a high-fat diet (HFD) as an experimental model of hypothyroidism (HT) with obesity.Methods:A total of 80 female wistar rats (5-months-old) were divided into eight different groups. Propylthiouracil (PTU) and HFD were used to induce the experimental HT and obesity, respectively. The euthyroid and hypothyroid rats were fed either normal chow or HFD with and without amla extract (AE, 100 mg/kg bw/day) for 6 weeks. The blood and tissues, liver and kidney OS and inflammatory parameters were studied using appropriate biochemical and molecular techniques.Results:PTU and HFD per se caused OS and inflammatory response as evidenced by increased plasma MDA, TNF-α, CRP and GPx in association with decreased levels of TAS and reduced glutathione (GSH). The proteomic analysis revealed that the expressions of pERK, pP38, TNF-α, IL6, COX2 and NOX-4 were up-regulated in the liver and kidney of these rats. In addition, all these metabolic derangements were further augmented when HT was followed by the addition of HFD. This suggested that there was a synergism between HT and the intake of HFD on the development of OS and inflammatory response.Conclusions:The treatment with amla fruit extract significantly restored the redox imbalance and inflammatory signaling and ameliorated OS and inflammatory response, suggesting the use of this natural compound as an alternative remedy or adjuvant for the management of metabolic complications concomitant with HT.

Download Full-text

Bromuconazole caused genotoxicity, hepatic and renal failure via oxidative stress process in Wistar rats

10.21203/rs.3.rs-420777/v1 ◽

2021 ◽

Author(s):

Karima RJIBA ◽

Hiba Hamdi ◽

Asma M’nassri ◽

Yosra Guedri ◽

Moncef Mokni ◽

...

Keyword(s):

Oxidative Stress ◽

Wistar Rats ◽

Corn Oil ◽

Protein Carbonyl ◽

Control Group ◽

Dependent Manner ◽

Glutathione S Transferase ◽

Male Wistar Rats ◽

Liver And Kidney ◽

Vegetables And Fruits

Abstract Bromuconazole is a triazole pesticide used to protect vegetables and fruits against diverse fungi pathologies. However, its utilization may be accompanied by diverse tissues injuries. For this, we tried to examine bromuconazole effects in liver and kidney tissues by the evaluation of biochemical and histopathological modifications also by genotoxic and oxidative stress analysis. Adult male Wistar rats were divided into four groups, each consisting of 6 animals. The control group received daily a corn oil (vehicle) orally. Three oral Bromuconazole doses were tested (1, 5 and 10 % of LD50) daily for 28 days. Bromuconazole increased the plasma activities of transaminases (AST, ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), creatinine and uric acid levels. histopathological check showed that Bromuconazole caused organs failure. This study make known that Bromuconazole caused conspicuous DNA damage either in hepatic and kidney tissues, with a significant increase in malondialdehyde and protein carbonyl levels followed by the increase in the enzymatic activity of catalase and superoxide dismutase in a dose dependent manner. Glutathione-S-transferase (GST) and peroxidase (GPx) activities were also recorded. Our results highlight that bromuconazole exposure induced genotoxic damage and organs failure that may be caused by the disturbances of oxidative stress statue in liver and kidney tissues.

Download Full-text

The beneficial effect of a phytonutrient-rich product against cadmium chloride-induced hepatorenal toxicity

Ukrainian Journal of Nephrology and Dialysis ◽

10.31450/ukrjnd.4(72).2021.04 ◽

2021 ◽

pp. 26-35

Author(s):

Omotayo Babatunde Ilesanmi ◽

Ridwan Abiodun Lawal

Keyword(s):

Oxidative Stress ◽

Cadmium Chloride ◽

Wistar Rats ◽

Hepatic Injury ◽

Hematological Parameters ◽

Intraperitoneal Administration ◽

Control Group ◽

Group I ◽

Male Wistar Rats ◽

Liver And Kidney

Abstract. This study was designed to investigate the hepatorenal protective effects of trévo, on cadmium-induced renal and hepatic injury in male Wistar rats. Methods. Fifteen healthy male Wistar rats were divided into three groups of five rats per group. Group I (control); group II (35mg/kg cadmium chloride (CdCl2); Group III (2 ml/kg trévo+ CdCl2. The rats were treated with trévo (2ml/kg orally) and administered CdCl2 3 hrs later. Twenty-four hours after the last administration rats were sacrificed and blood was collected via cardiac puncture and processed for hematological parameters and assessment of urea, creatinine (CREA), and uric acid (UA), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and albumin (ALB). The liver and kidney were excised and processed for markers of oxidative stress. Results intraperitoneal administration of 35 mg/kg of CdCl2 caused a significant increase in serum concentration of urea, CREA, UA, AST, ALT, while the concentration of ALB was significantly lower (P<0.0001). CdCl2 caused a significant reduction in packed cell volume, hemoglobin while the total white blood cell count, neutrophils, lymphocytes, monocytes, eosinophils, and basophils were increased. Oxidative stress was significantly pronounced in the liver and kidney of rats exposed to CdCl2 as observed in the high concentration of malondialdehyde, decreased concentration of glutathione, the activity of catalase, superoxide dismutase, and glutathione-S-transferase. Pretreatment with trévo was able to significantly prevent the anemic, oxidative damage, renal and hepatic injury initiated by CdCl2. Conclusions. The study reveals that trévo is effective in attenuating cadmium-induced hepatorenal toxicity in male Wistar rats.

Download Full-text

Behavioural and biochemical effects of one-week exposure to aflatoxin B1 and aspartame in male Wistar rats

World Mycotoxin Journal ◽

10.3920/wmj2018.2424 ◽

2019 ◽

Vol 12 (3) ◽

pp. 293-305

Author(s):

N.S. Souto ◽

M. Dassi ◽

A.C.M. Braga ◽

E.V.F. Rosa ◽

M.R. Fighera ◽

...

Keyword(s):

Oxidative Stress ◽

Wistar Rats ◽

Artificial Sweetener ◽

Glutathione S Transferase ◽

Obsessive Compulsive ◽

Male Wistar Rats ◽

Markers Of Oxidative Stress ◽

Liver And Kidney ◽

Aflatoxin B ◽

Gst Activity

Food products are susceptible to contamination by mycotoxins, and aflatoxin B1 (AFB1) stands as the most toxic among them. AFB1 intoxication results in distinct signs, including widespread systemic toxicity. Aspartame (ASP) is an artificial sweetener used as a sugar substitute in many products, and compelling evidence indicates ASP can be toxic. Interestingly, mechanisms underlying ASP and AFB1 toxicity involve oxidative stress. In this context, concomitant use of ASP and AFB1 in a meal may predispose to currently unidentified behavioural and biochemical changes. Therefore, we evaluated the effect of AFB1 (250 μg/kg, intragastrically (i.g.)) and/or ASP (75 mg/kg, i.g.) exposure for 7 days on behavioural and biochemical markers of oxidative stress in male Wistar rats. AFB1 and/or ASP increased hepatic glutathione S-transferase (GST) activity when compared to controls. In the kidneys, increased GST activity was detected in AFB1 and AFB1+ASP groups. In addition, AFB1 and or ASP elicited behavioural changes in the open field, marble burying and splash tests, however no additive effects were detected. Altogether, present data suggest AFB1 and ASP predispose to anxiety- and obsessive-compulsive-like symptoms, as well as to enzymatic defence system imbalance in liver and kidney of Wistar rats.

Download Full-text