Kolaviron Ameliorates 7, 12-Dimethylbenzanthracene - Induced Mammary Damage in Female Wistar Rats

2021 ◽  
Vol 21 ◽  
Author(s):  
Rabiatu B. Suleiman ◽  
Aliyu Muhammad ◽  
Ismaila A. Umara ◽  
Mohammed A. Ibrahima ◽  
Ochuko L. Erukainure ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Sialic Acid ◽  
Wistar Rats ◽  
Mrna Level ◽  
Estrogen Receptor Α ◽  
Tumor Formation ◽  
Mammary Tissue ◽  
Standard Drug ◽  
Female Wistar Rats ◽  
Α Level

Background: Kolaviron (KV) is a flavonoid rich portion obtained from Garcinia kola seeds with a number of reported pharmacological effects. However, its ameliorative effects on 7,12-Dimethylbenzanthracene (DMBA)-induced mammary damage has not been fully investigated, despite the reported use of the seeds in the treatment of inflammatory related disorders. Objective: To evaluate the ameliorative effects of KV on DMBA-induced mammary damage in female Wistar rats. Methods: Forty-nine (49) female Wistar rats were randomly assigned into seven groups of seven rats each. DMBA was administered orally to rats in five of the groups as a single dose of 80 mg/kg body wt while the remaining two groups received the vehicle. The rats were palpated weekly for 3 months to monitor tumor formation. After 3 months of DMBA administration, 1 ml of blood was collected to assay for estrogen receptor- α (ER-α) level. Thereafter, the vehicle (dimethyl sulfoxide) was daily administered to the negative control and positive control groups for the 14 days duration of the experiment while three groups were each given a daily oral dose of 50, 100 and 200 mg/kg body wt of KV for the duration of the experiment. The last DMBA-induced group received 10 mg/kg body wt of the standard drug tamoxifen twice in a week and the remaining DMBA-free group received 200 mg/kg body wt KV. Subsequently, the animals were humanly sacrificed and ER-α, sialic acids, sialidase, sialyltransferase levels were assay for in blood and mammary tissues followed by histopathological examinations. Results: Significantly higher levels of estrogen receptor-α (ER-α), formation of lobular neoplastic cells, epithelial hyperplasia, lymphocyte infiltration and increased sialylation were detected in DMBA-induced rats. Treatment with KV at 50, 100 and 200 mg/kg body weight resulted in a significant (p<0.05) decrease in ER-α level, significantly (p<0.05) lower free serum sialic acid (21.1%), total sialic acid level of the mammary tissue (21.57%), sialyltransferase activity (30.83%) as well as mRNA level of the sialyltransferase gene (ST3Gal1) were observed after KV interventions. Conclusion: The findings suggest that KV could be further explored in targeting DMBA-induced mammary damage implicated in mammary carcinogenesis.

Effects of tamoxifen on estrogen receptor-α level in immune cells and humoral specific response after immunization of C3H/He male mice with syngeneic testicular germ cells (TGC)

Autoimmunity ◽  
2011 ◽  
Vol 44 (6) ◽  
pp. 520-530 ◽  
Author(s):  
Tomasz Maj ◽  
Kinga Switała-Jelen ◽  
Arkadiusz Miazek ◽  
Beata Szafarowicz-Basta ◽  
Liliana Kiczak ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Germ Cells ◽  
Immune Cells ◽  
Estrogen Receptor Α ◽  
Specific Response ◽  
Male Mice ◽  
Α Level

Nelumbo nucifera Leaves Prevent NMU-Induced Mammary Tumor through Downregulation of Fatty Acid Synthase, Estrogen Receptor-α and Her2 Expression

2019 ◽  
Vol 47 (08) ◽  
pp. 1885-1899
Author(s):  
Li-Sung Hsu ◽  
Chun-Hua Chang ◽  
Yi-Ju Lee ◽  
Chau-Jong Wang
Keyword(s):  
Estrogen Receptor ◽  
Fatty Acid ◽  
Cancer Cells ◽  
Nude Mice ◽  
Mammary Tumor ◽  
Fatty Acid Synthase ◽  
Fatty Acid Synthetase ◽  
Estrogen Receptor Α ◽  
Tumor Formation ◽  
Nelumbo Nucifera

Diet polyphenol can reportedly prevent the formation of breast-cancer cells. Nelumbo nucifera leaf extract (NLE) is enriched with polyphenols and has several cellular functions, such as anti-atherosclerosis, anti-inflammation, and antitumor. In this study, we investigated the role of NLE in the prevention of N-methyl-N-nitrosourea (NMU)-induced mammary tumor formation. Cotreatment with NLE significantly reduced the NMU-induced tumor incidence, number, and volume. NLE administration significantly repressed the tumor growth and weight of nude mice upon inoculation with BT-474 cancer cells. Immunohistochemical staining indicated that fatty acid synthetase, estrogen receptor (ER)-[Formula: see text], and phosphorylated ER-[Formula: see text] were obviously reduced in the cancer part of BT-474 inoculated nude mice upon administration of 2% NLE. Western blot analysis revealed that NLE and NLPE (polyphenol-rich NLE) repressed ER-[Formula: see text] expression and phosphorylation and decreased the phosphorylation of Her-2 without affecting their expression. Overall, NLE and NLPE exhibited more effective antitumor abilities in NMU-induced mammary cancer formation than with tamoxifen and Herceptin.

Thioacetamide differentially affects the expression and activity of glutathione-S-transferase in the liver of Wistar rats

2004 ◽  
Vol 23 (9) ◽  
pp. 431-437
Author(s):  
Murilo Tadeu Domingues Bueno ◽  
Beny Spira
Keyword(s):  
Single Dose ◽  
Protein Level ◽  
Wistar Rats ◽  
Mrna Level ◽  
Female Rats ◽  
Glutathione S Transferase ◽  
Female Wistar Rats ◽  
Different Sources ◽  
Gst Activity ◽  
Expression And Activity

Glutathione-S-transferase (GST) is a family of enzymes involved in the detoxification of toxic and carcinogenic compounds. In the present study, the effect of thioacetamide (TA), a hepatotoxic and hepatocarcinogenic compound, on the activity and expression of GST of Wistar female rats was tested. Animals were treated with a single dose of TA (250 mg/kg) for 12, 24, 48 and 72 hours. GST activity toward the broad substrate 1-chloro-2,4-dinitrobenzene was enhanced by TA. The protein level of the GST classes alpha and mu as well as the mRNA level of several GST subunits were also positively affected by the TA treatment. Female Wistar rats of the same age but from two other different colonies had their GST activity either inhibited or not affected by TA. The basal mRNA level of class alpha and class mu GST was also tested in female Wistar rats obtained from five different sources. Differences in the basal level of class alpha mRNA were observed in rats from at least three different sources, while class mu mRNA level was distinct in two groups of animals.

scholarly journals Effects of Xylopia aethiopica Pod Extract on Reproductive Hormones in Female Wistar Rats

2021 ◽  
pp. 1-9
Author(s):  
C. G. Okonkwo ◽  
H. A. Ogbunugafor ◽  
A. A. Oladejo
Keyword(s):  
Luteinizing Hormone ◽  
Wistar Rats ◽  
Reproductive Hormones ◽  
Female Rats ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Standard Drug ◽  
Significant Difference ◽  
Female Wistar Rats ◽  
Xylopia Aethiopica

Plants and their metabolites have been used in the treatment of various disease conditions from time immemorial. This study investigated the effect of hydro-ethanolic extract of pods of Xylopia aethiopica on reproductive hormone (follicle stimulating hormone (FSH), luteinizing hormone (LH) and progesterone) in female Wistar rats. Thirty female wistar rats were randomly divided into 5 groups of six animals per group, per cage (n=6). Group A served as the normal control group and was administered distilled water while group B served as positive control and was administered 5 mg of standard drug (Clomid-Clomiphene Citrate). Groups C, D and E served as the treatment group and was administered 50 mg/kg, 100 mg/kg and 200 mg/kg extract of X. aethiopica respectively for seven days. Administration of drugs and extract was done via oral route. The rats were thereafter copulated with their male counterparts for 72 hours and the female rats were observed throughout their gestation and the numbers of litters produced were recorded. The hormonal assay was carried out using Enzyme Linked Immunosorbent Assay. Result showed a significant increase in FSH and Progesterone level (p<0.05) in treated group when compared with the control group while no significant difference (p>0.05) was observed for the luteinizing hormone in the treated groups. The work thus showed that the plant could exert a positive effect on female fertility by enhancing levels of female reproductive hormones.

Uterine effects of Genistein and selective estrogen receptor agonists in female Wistar rats

2007 ◽  
Vol 115 (S 1) ◽  
Author(s):  
F Möller ◽  
A Schwabe ◽  
O Zierau ◽  
T Hertrampf ◽  
J Seibel ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Wistar Rats ◽  
Receptor Agonists ◽  
Female Wistar Rats

scholarly journals Decreased Expression of Wnt7a mRNA Is Inversely Associated with the Expression of Estrogen Receptor-α in Human Uterine Leiomyoma

2001 ◽  
Vol 86 (1) ◽  
pp. 454-457
Author(s):  
Shuanfang Li ◽  
Tung-Chin Chiang ◽  
Gloria R. Davis ◽  
Rachele M. Williams ◽  
Valerie P. Wilson ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Uterine Leiomyoma ◽  
Molecular Mechanisms ◽  
Reproductive Tract ◽  
Mrna Level ◽  
Critical Role ◽  
Estrogen Receptor Α ◽  
Female Reproductive Tract ◽  
Inverse Association ◽  
Sex Steroid Hormone

Wnt-7a gene not only guides the development of the anterior-posterior axis in the female reproductive tract, but also plays a critical role in uterine smooth muscle pattering and maintenance of adult uterine function. This gene is also responsive to changes in the levels of sex steroid hormone in the female reproductive tract. To explore the molecular mechanisms underlying the pathogenesis of uterine leiomyoma, the expression of Wnt7a mRNA in the leiomyoma has been assessed. RT-PCR was performed on uterine leiomyomas and the adjacent myometria. Of 30 cases of leiomyomas studied, 67% showed a decreased mRNA level as compared to the paired myometria. On the other hand, estrogen receptor-α (ER-α) mRNA is hyper-expressed in 67% of the leiomyomas as compared to their paired myometrium. An inverse association at mRNA expression was found between Wnt7a and ER-α. Miller et alhas shown that fetal exposure of DES results in de-regulation of Wnt7a during uterine morphogenesis. Referring to their results, we have postulated that hypersensitivity of leiomyoma cells to estrogen may deregulate the Wnt7a expression. Decreased expression of Wnt7a may lead to loss of control in patterning of the myometrium and result in development of leiomyoma

scholarly journals Validation of transgenic models of breast cancer: ductal carcinoma in situ (DCIS) and Brca1-mutation-related breast cancer

2005 ◽  
Vol 8 (8) ◽  
Author(s):  
M. S. Frech ◽  
L. P. Jones ◽  
P. A. Furth
Keyword(s):  
Breast Cancer ◽  
Estrogen Receptor ◽  
Mouse Model ◽  
Mouse Models ◽  
Ductal Carcinoma ◽  
Brca1 Mutation ◽  
Tumor Development ◽  
Estrogen Receptor Α ◽  
Mammary Tissue

Available mouse models of ductal carcinomain situ(DCIS) and BRCA1-mutation-related breast cancer are reviewed. The best validated mouse models of human DCIS are the conditional estrogen receptor α in mammary tissue (CERM) model initiated by deregulated estrogen receptor α and the serial explant mouse model initiated by p53 deficiency. At present the most useful and best validated mouse model of BRCA1-mutation-related breast cancer uses the cre-lox system to make a conditional Brca1 deletion targeted to mammary epithelial cells. The major shortcoming of the non-conditional Brca1 models is the high incidence of non-mammary tumor development. The use of mammary gland transplants or explants from these mice into nude hosts is one approach that could be used to circumvent this deficiency. Development and validation of a Brca1-mutation-related mouse model of basal cell breast cancer is an important next step.

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