scholarly journals Host‐specific preference of some Flavobacterium psychrophilum multilocus sequence typing genotypes determines their ability to cause bacterial coldwater disease in coho salmon ( Oncorhynchus kisutch )

2021 ◽  
Author(s):  
Christopher Knupp ◽  
Matti Kiupel ◽  
Travis O. Brenden ◽  
Thomas P. Loch
Keyword(s):  
Multilocus Sequence Typing ◽  
Coho Salmon ◽  
Oncorhynchus Kisutch ◽  
Flavobacterium Psychrophilum ◽  
Specific Preference ◽  
Coldwater Disease ◽  
Bacterial Coldwater Disease

Phenotypic and genotypic analysis of Flavobacterium psychrophilum isolates from Ontario salmonids with bacterial coldwater disease

2008 ◽  
Vol 54 (8) ◽  
pp. 619-629 ◽  
Author(s):  
Shohreh Hesami ◽  
Katie J. Allen ◽  
Devon Metcalf ◽  
Vaughn E. Ostland ◽  
Janet I. MacInnes ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Rainbow Trout ◽  
16S Rrna ◽  
Sequence Type ◽  
Chain Reaction ◽  
Flavobacterium Psychrophilum ◽  
Genotypic Analysis ◽  
Polymerase Chain ◽  
Coldwater Disease ◽  
Bacterial Coldwater Disease

Flavobacterium psychrophilum is the causative agent of bacterial coldwater disease (BCWD) and rainbow trout fry syndrome. BCWD has a considerable economic impact on aquaculture operations in Ontario, Canada, and our limited understanding of the population structure and epidemiology of F. psychrophilum isolates is an impediment to the development of improved management strategies. Seventy-five 16S rRNA gene and gyr polymerase chain reaction positive isolates of F. psychrophilum that had been collected over a 16-year period from farmed salmonids with tail rot, necrotic myositis, and osteochondrosis were characterized morphologically, biochemically, and genotypically. Although the isolates were homogeneous by preliminary biochemical and phenotypic characterization, two distinct biovars were found by API ZYM testing. As well, four restriction pattern types were detected by 16S rRNA polymerase chain reaction – restriction fragment length polymorphism analysis and there was a significant (P < 0.001) correlation between biovar I and digestion with MaeIII and between biovar II and digestion with MnlI or no site (P < 0.05). Further heterogenity was detected by sequence analysis of a 194 bp stem loop 3 region of rRNA. Nine sequence types were identified; 40/46 biovar I isolates were sequence type “a”, while 21/32 biovar II isolates belonged to either sequence type “c” or “d”. More than one biovar and genotype was identified among the strains recovered from separate fish sampled from three groups of rainbow trout ( Oncorhynchus mykiss ) experiencing BCWD mortality events. No association was found between genotype or biovar and type of disease. Taken together, these data suggest that F. psychrophilum from Ontario can be grouped into two major lineages based on biovar and 16S rRNA polymorphisms, and although three major strain types were most frequently isolated in this study, it appears that the population of F. psychrophilum with pathogenic potential is quite heterogeneous.

scholarly journals Large-Scale Analysis ofFlavobacterium psychrophilumMultilocus Sequence Typing Genotypes Recovered from North American Salmonids Indicates that both Newly Identified and Recurrent Clonal Complexes Are Associated with Disease

2019 ◽  
Vol 85 (6) ◽  
Author(s):  
Christopher Knupp ◽  
Gregory D. Wiens ◽  
Mohamed Faisal ◽  
Douglas R. Call ◽  
Kenneth D. Cain ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Rainbow Trout ◽  
North America ◽  
North American ◽  
Economic Losses ◽  
Content Type ◽  
Flavobacterium Psychrophilum ◽  
Coldwater Disease ◽  
Rainbow Trout Fry Syndrome ◽  
Bacterial Coldwater Disease

ABSTRACTFlavobacterium psychrophilum, the etiological agent of bacterial coldwater disease (BCWD) and rainbow trout fry syndrome (RTFS), causes significant economic losses in salmonid aquaculture, particularly in rainbow trout (Oncorhynchus mykiss). Prior studies have used multilocus sequence typing (MLST) to examine genetic heterogeneity withinF. psychrophilum. At present, however, its population structure in North America is incompletely understood, as only 107 isolates have been genotyped. Herein, MLST was used to investigate the genetic diversity of an additional 314 North AmericanF. psychrophilumisolates that were recovered from ten fish host species from 20 U.S. states and 1 Canadian province over nearly four decades. These isolates were placed into 66 sequence types (STs), 47 of which were novel, increasing the number of clonal complexes (CCs) in North America from 7 to 12. Newly identified CCs were diverse in terms of host association, distribution, and association with disease. The largestF. psychrophilumCC identified was CC-ST10, within which 10 novel genotypes were discovered, most of which came fromO. mykissexperiencing BCWD. This discovery, among others, provides evidence for the hypothesis that ST10 (i.e., the founding ST of CC-ST10) originated in North America. Furthermore, ST275 (in CC-ST10) was recovered from wild/feral adult steelhead and marks the first recovery of CC-ST10 from wild/feral fish in North America. Analyses also revealed that at the allele level, the diversification ofF. psychrophilumin North America is driven three times more frequently by recombination than random nucleic acid mutation, possibly indicating how new phenotypes emerge within this species.IMPORTANCEFlavobacterium psychrophilumis the causative agent of bacterial coldwater disease (BCWD) and rainbow trout fry syndrome (RTFS), both of which cause substantial losses in farmed fish populations worldwide. To better prevent and control BCWD and RTFS outbreaks, we sought to characterize the genetic diversity of several hundredF. psychrophilumisolates that were recovered from diseased fish across North America. Results highlighted multipleF. psychrophilumgenetic strains that appear to play an important role in disease events in North American aquaculture facilities and suggest that the practice of trading fish eggs has led to the continental and transcontinental spread of this bacterium. The knowledge generated herein will be invaluable toward guiding the development of future disease prevention techniques.

scholarly journals Bacterial coldwater disease of fishes caused by Flavobacterium psychrophilum

2011 ◽  
Vol 2 (2) ◽  
pp. 97-108 ◽  
Author(s):  
Clifford E. Starliper
Keyword(s):  
Flavobacterium Psychrophilum ◽  
Coldwater Disease ◽  
Bacterial Coldwater Disease

scholarly journals Pathogenic profile of Flavobacterium psychrophilum, the causative bacterium of bacterial coldwater disease in ayu Plecoglossus altivelis

2002 ◽  
Vol 68 (sup2) ◽  
pp. 1251-1252
Author(s):  
MOTOKI KONDO ◽  
KENJI KAWAI ◽  
KENROU KUROHIARA ◽  
SHUN-ICHIROU OSHIMA
Keyword(s):  
Plecoglossus Altivelis ◽  
Flavobacterium Psychrophilum ◽  
Coldwater Disease ◽  
Bacterial Coldwater Disease ◽  
Causative Bacterium

scholarly journals Complete Genome Sequence of Flavobacterium psychrophilum Strain OSU THCO2-90, Used for Functional Genetic Analysis

2017 ◽  
Vol 5 (8) ◽  
Author(s):  
Tatiana Rochat ◽  
Paul Barbier ◽  
Pierre Nicolas ◽  
Valentin Loux ◽  
David Pérez-Pascual ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Coho Salmon ◽  
Oncorhynchus Kisutch ◽  
Open Reading Frames ◽  
Circular Chromosome ◽  
Content Type ◽  
Flavobacterium Psychrophilum ◽  
Reading Frames

ABSTRACT We report here the complete annotated genome sequence of Flavobacterium psychrophilum OSU THCO2-90, isolated from Coho salmon (Oncorhynchus kisutch) in Oregon. The genome consists of a circular chromosome with 2,343 predicted open reading frames. This strain has proved to be a valuable tool for functional genomics.

scholarly journals The Resistance of Ayu Plecoglossus altivelis Recovered from Bacterial Coldwater Disease against Reinfection with Flavobacterium psychrophilum

2007 ◽  
Vol 42 (3) ◽  
pp. 159-161 ◽  
Author(s):  
Hiroaki Kintsuji ◽  
Michitaka Yamamoto ◽  
Koji Ninomiya
Keyword(s):  
Plecoglossus Altivelis ◽  
Flavobacterium Psychrophilum ◽  
Coldwater Disease ◽  
Bacterial Coldwater Disease

scholarly journals Draft Genome Sequences of Three Flavobacterium psychrophilum Strains Isolated from Coldwater Disease Outbreaks at Three Production Hatcheries: TABLE 1 

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Regg Neiger ◽  
Milton Thomas ◽  
Seema Das ◽  
Michael Barnes ◽  
Brian Fletcher ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
South Dakota ◽  
Draft Genome ◽  
Disease Outbreaks ◽  
Genome Sequences ◽  
Flavobacterium Psychrophilum ◽  
Coldwater Disease ◽  
Bacterial Coldwater Disease ◽  
Strain Genome

We report here the genome sequences of three Flavobacterium psychrophilum strains causing a bacterial coldwater disease (BCWD) outbreak, isolated from infected rainbow trout from hatcheries in Montana and South Dakota. The availability of these virulent outbreak-causing strain genome sequences will help further understand the pathogenesis of BCWD.

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